Silvestroff, L.; Bartucci, S.; Soto, E.; Gallo, V.; Pasquini, J.; Franco, P. "Cuprizone-Induced demyelination in CNP::GFP transgenic mice" (2010) Journal of Comparative Neurology. 518(12):2261-2283
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Cuprizone (bis-cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′-3′-cyclic nucleotide 3′-phosphodies-terase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well-known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone-treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. © 2010 Wiley-Liss, Inc.


Documento: Artículo
Título:Cuprizone-Induced demyelination in CNP::GFP transgenic mice
Autor:Silvestroff, L.; Bartucci, S.; Soto, E.; Gallo, V.; Pasquini, J.; Franco, P.
Filiación:Departamento de Química Biológica, IQUIFIB and IIMHNO, UBA-CONICET, Junín 956, Buenos Aires C1113AAD, Argentina
Center for Neuroscience Research, Children's National Medical Center, Washington, DC 20010, United States
Palabras clave:Cuprizone; Demyelination; Oligodendrocytes; Remyelination; 2',3' cyclic nucleotide 3' phosphodiesterase; cuprizone; green fluorescent protein; myelin basic protein; adult animal; animal cell; animal cell culture; animal experiment; animal model; animal tissue; anterior commissure; antibody specificity; article; astrocytosis; brain cortex; brain stem; caudate nucleus; cell activation; cell damage; central nervous system; controlled study; corpus callosum; demyelination; hippocampus; immunohistochemistry; microglia; microphotography; microscopy; mouse; nonhuman; olfactory bulb; oligodendroglia; optic chiasm; priority journal; protein expression; putamen; quantitative analysis; tissue section; transgenic mouse; 2',3'-Cyclic-Nucleotide Phosphodiesterases; Animals; Astrocytes; Brain; Cell Count; Cell Proliferation; Cuprizone; Demyelinating Diseases; Glial Fibrillary Acidic Protein; Gliosis; Green Fluorescent Proteins; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microglia; Myelin Basic Proteins; Neurons; Oligodendroglia; Promoter Regions, Genetic
Página de inicio:2261
Página de fin:2283
Título revista:Journal of Comparative Neurology
Título revista abreviado:J. Comp. Neurol.
CAS:2',3' cyclic nucleotide 3' phosphodiesterase, 60098-35-3; cuprizone, 370-81-0; 2',3'-Cyclic-Nucleotide Phosphodiesterases, 3.1.4.-; Cuprizone, 370-81-0; Glial Fibrillary Acidic Protein; Green Fluorescent Proteins, 147336-22-9; Myelin Basic Proteins


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---------- APA ----------
Silvestroff, L., Bartucci, S., Soto, E., Gallo, V., Pasquini, J. & Franco, P. (2010) . Cuprizone-Induced demyelination in CNP::GFP transgenic mice. Journal of Comparative Neurology, 518(12), 2261-2283.
---------- CHICAGO ----------
Silvestroff, L., Bartucci, S., Soto, E., Gallo, V., Pasquini, J., Franco, P. "Cuprizone-Induced demyelination in CNP::GFP transgenic mice" . Journal of Comparative Neurology 518, no. 12 (2010) : 2261-2283.
---------- MLA ----------
Silvestroff, L., Bartucci, S., Soto, E., Gallo, V., Pasquini, J., Franco, P. "Cuprizone-Induced demyelination in CNP::GFP transgenic mice" . Journal of Comparative Neurology, vol. 518, no. 12, 2010, pp. 2261-2283.
---------- VANCOUVER ----------
Silvestroff, L., Bartucci, S., Soto, E., Gallo, V., Pasquini, J., Franco, P. Cuprizone-Induced demyelination in CNP::GFP transgenic mice. J. Comp. Neurol. 2010;518(12):2261-2283.