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Abstract:

The effect of different parameters on the interfacial polarity of phospholipid vesicles was studied using two polarity-sensitive fluorescent probes, dansyldihexadecylamine (DA) and dansylphosphatidylethanolamine (DPE). The analysis was based on the Stokes shift and steady-state fluorescence anisotropy changes with different parameters. The influence of surface curvature, lipid composition, and surface charge density was analyzed in small and large unilamellar vesicles and in multilamellar vesicles at different temperatures and in different lipid phase states. The membranes showed significant polarity, packing constraints, and hydration degree changes at the polar head level when different variables were imposed. The comparative analysis of the probe response demonstrates that each dansyl derivative yields different information about membrane changes. DA appears to be more sensitive to packing constraints and hydration, probably due to its molecular structure and location at the interface level. DPE seems to sense polarity values averaged in the region where its polar head can pivot, and is, as expected, susceptible to electrostatic interactions.

Registro:

Documento: Artículo
Título:Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes
Autor:Bernik, D.L.; Martín Negri, R.
Filiación:Catedra de Quim. Gen. e Inorg., Fac. de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 2Piso, 1113 Buenos Aires, Argentina
INQUIMAE, Depto. de Quim. Inorgánica, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Dansyl probes; Interfaces; Liposomes; Micropolarity; Stokes shift; dansyl chloride; dansyldihexadecylamine; dansylphosphatidylethanolamine; liposome; unclassified drug; anisotropy; article; fluorescence; hydration; lipid composition; lipid vesicle; priority journal; temperature
Año:1998
Volumen:203
Número:1
Página de inicio:97
Página de fin:105
DOI: http://dx.doi.org/10.1006/jcis.1998.5522
Título revista:Journal of Colloid and Interface Science
Título revista abreviado:J. Colloid Interface Sci.
ISSN:00219797
CODEN:JCISA
CAS:dansyl chloride, 605-65-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219797_v203_n1_p97_Bernik

Referencias:

  • Cevc, G., Marsh, D., (1983) J. Phys. Chem., 87, p. 376
  • Cevc, G., (1990) Biochim. Biophys. Acta., 1031 (3), p. 311
  • McLaughlin, S., (1989) Annu. Rev. Biophys. Chem., 18, p. 113
  • Onsager, L., (1936) J. Am. Chem. Soc., 58, p. 1486
  • Bayliss, N.S., (1950) J. Chem. Phys., 18, p. 292
  • Lippert, E., (1955) Naturforsch. A., 10, p. 541. , (a)
  • Lippert, E., (1957) Z. Elektrochem., 61, p. 962
  • Mataga, N., Kaifu, Y., Koizumi, M., (1956) Bull. Chem. Soc. Jpn., 29, p. 465
  • McRae, E.G., (1957) J. Phys. Chem., 61, p. 562
  • Bhaumik, M.L., Hardwick, R., (1963) J. Chem. Phys., 39, p. 1595
  • Marcus, R.A., (1963) J. Chem. Phys., 38, p. 1858
  • Marcus, R.A., (1963) J. Chem. Phys., 39, p. 1734
  • Marcus, R.A., (1965) J. Chem. Phys., 43, p. 1261
  • Van Der Zwan, G., Hynes, J.T., (1985) J. Phys. Chem., 89, p. 4181
  • Böttcher, C.J.F., Bordewijk, P., (1978) In, Theory of Electric Polarization, 2. , Elsevier, Amsterdam
  • Ohki, S., Arnold, K., (1990) J. Membr. Biol., 114, p. 195
  • Kimura, Y., Ikegami, A., (1985) J. Membr. Biol., 85, p. 225
  • MacDonald, D.C., MacDonald, R.I., Menco, B., Taleshita, K., Subbarao, N.K., Hu, L., (1991) Biochim. Biophys. Acta, 1061, p. 297
  • Fiske, C., Subarow, Y., (1925) J. Biol. Chem., 66, p. 375
  • Aramendía, P.F., Negri, R.M., San Román, E., (1994) J. Phys. Chem., 98, p. 3165
  • De Backer, S., Negri, R.M., De Feyter, S., Dutt, B.G., Amelot, M., De Schryver, F.C., Müllen, K., Holtrup, F., (1995) Chem. Phys. Lett., 233, p. 538
  • Lide, D.R., (1991) Handbook of Chemistry and Physics, , CRC, Boca Raton, FL
  • Sixl, F., Watts, A., (1982) Biochemistry, 21, p. 6446
  • Blume, A., (1983) Biochemistry, 22, p. 5436
  • Bechinger, B., Seelig, J., (1991) Biochem., 30, p. 3923
  • Cafiso, D.S., (1995) Permeability and Stability of Lipid Bilayers, , Boca Raton: CRC
  • Mazurenko, Y.T., Bakhshiev, N.G., (1970) Opt. Spectrosk., 28, p. 490
  • Stubbs, C.D., Meech, S.R., Lee, A.G., Phillips, D., (1985) Biochim. Biophys. Acta., 815, p. 351
  • Ghiggino, K., Lee, A.G., Meech, S.R., O'Connor, D.V., Phillips, D., (1981) Biochemistry, 20, p. 5381
  • Simon, S.A., McIntosh, T.J., (1986) Proc. Nat. Acad. Sci. USA, 86, p. 9263
  • Faucon, J.F., Lussan, C., (1973) Biochim. Biophys. Acta, 307, p. 459
  • Lakowicz, J.R., (1983) Principles of Fluorescence Spectroscopy, , New York: Plenum
  • Dufourc, E.J., Mayer, C., Stohrer, J., Althoff, G., Kothe, G., (1992) Biophys. J., 61, p. 42
  • Lafrance, D., Marion, D., Pezolet, M., (1990) Biochemistry, 29, p. 4592
  • Sonnenschein, M.F., Weiss, R.G., (1990) Photochem. Photobiol., 51, p. 539
  • Lissi, E.A., Abuin, E., Saez, M., Zanocco, A., Disalvo, E.A., (1992) Langmuir, 8, p. 348
  • Huang, C., Mason, J.Y., (1978) Proc. Natl. Acad. Sci., 75, p. 308
  • Shepherd, J.C.W., Buldt, G., (1978) Biochim. Biophys. Acta, 514, p. 83
  • MacDonald, P.M., Leisen, J., Marassi, F.M., (1991) Biochemistry, 30, p. 3558
  • Pascher, I., Sundell, S., Harlos, K., Eibl, H., (1987) Biochim. Biophys. Acta, 896, p. 77
  • Watts, A., Harlos, K., Marsh, D., (1981) Biochim. Biophys. Acta, 645, p. 91
  • McIntosh, T.J., Magid, A.D., Simon, S.A., (1989) Biochemistry, 28, p. 7904
  • Saito, H., Araiso, T., Shirahama, H., Koyama, T., (1991) J. Biochem., 109, p. 559
  • Needham, T., McIntosh, T.J., Evans, E., (1988) Biochemistry, 27, p. 4668
  • Neal, S.L., Villegas, M.M., (1995) Anal. Chem., 67, p. 2659

Citas:

---------- APA ----------
Bernik, D.L. & Martín Negri, R. (1998) . Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes. Journal of Colloid and Interface Science, 203(1), 97-105.
http://dx.doi.org/10.1006/jcis.1998.5522
---------- CHICAGO ----------
Bernik, D.L., Martín Negri, R. "Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes" . Journal of Colloid and Interface Science 203, no. 1 (1998) : 97-105.
http://dx.doi.org/10.1006/jcis.1998.5522
---------- MLA ----------
Bernik, D.L., Martín Negri, R. "Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes" . Journal of Colloid and Interface Science, vol. 203, no. 1, 1998, pp. 97-105.
http://dx.doi.org/10.1006/jcis.1998.5522
---------- VANCOUVER ----------
Bernik, D.L., Martín Negri, R. Local polarity at the polar head level of lipid vesicles using dansyl fluorescent probes. J. Colloid Interface Sci. 1998;203(1):97-105.
http://dx.doi.org/10.1006/jcis.1998.5522