Sauer, J.; Renner, U.; Hopfner, U.; Lange, M.; Müller, A.; Strasburger, C.J.; Pagotto, U.; Arzt, E.; Stalla, G.K. "Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures" (1998) Journal of Clinical Endocrinology and Metabolism. 83(7):2429-2434
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In addition to the well-known modulation of immune and inflammatory responses, the interleukin-1 (IL-1) system has been shown to be involved in the regulation of anterior pituitary hormone secretion and growth. We previously demonstrated that IL-1 receptor antagonist (IL-1ra) is expressed in human pituitary adenomas cultured in vitro. In the present study, we investigated the regulation of IL-1ra protein by IL-1β (1-100 U/mL) in human somatotroph adenomas (n = 9) cultured for 12-48 h. IL-1β significantly enhanced the concentration of IL-1ra dose dependently in the somatotroph adenoma cell lysates, whereas IL-1ra concentrations remained unchanged in the culture supernatants. Furthermore, basal IL-1ra concentrations were significantly higher in the cell lysates compared with the corresponding culture supernatants. The regulation of IL-1ra in somatotroph adenoma cells is different from human cultured monocytes, in which IL-1β significantly stimulated IL-1ra secretion into the culture supernatants, and no change of intracellular IL-1ra content was observed. Incubation of the somatotroph adenoma cells with 100 U/mL IL-1β did not result in a change of GH concentrations in the culture supernatants. Enhancement of intracellular IL- 1ra protein by IL-1β may represent a mechanism intrinsic to somatotroph adenoma cells to counterregulate the response to IL-1β on hormone secretion or cellular growth.


Documento: Artículo
Título:Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures
Autor:Sauer, J.; Renner, U.; Hopfner, U.; Lange, M.; Müller, A.; Strasburger, C.J.; Pagotto, U.; Arzt, E.; Stalla, G.K.
Filiación:Max-Planck-Institute of Psychiatry, Department of Endocrinology, Munich, Germany
Division of Endocrinology, Department of Medicine, University of Essen, Germany
Lab. Fisiol. y Biol. Molecular, Naturales University of Buenos Aires, Consejo Nac. de Invest. Cie. y Tec., Buenos Aires, Argentina
Department of Neurosurgery, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany
Department of Neurosurgery, University of Munich, D-80804 Munich, Germany
Department of Medicine, University of Munich, D-80804 Munich, Germany
Max Planck Institute of Psychiatry, Department of Endocrinology, Kraepelinstrasse 10, D-80804 Munich, Germany
Palabras clave:growth hormone; interleukin 1 receptor blocking agent; interleukin 1beta; adult; aged; article; cell culture; cell growth; clinical article; concentration response; cytokine release; female; gene expression regulation; growth hormone blood level; growth hormone release; hormone release; human; human tissue; hypophysis adenoma; male; priority journal; somatic cell; supernatant; tissue level; Adenoma; Adult; Aged; Cells, Cultured; Female; Humans; Interleukin-1; Male; Middle Aged; Monocytes; Pituitary Neoplasms; Receptors, Interleukin-1; Tumor Cells, Cultured
Página de inicio:2429
Página de fin:2434
Título revista:Journal of Clinical Endocrinology and Metabolism
Título revista abreviado:J. Clin. Endocrinol. Metab.
CAS:Interleukin-1; Receptors, Interleukin-1


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---------- APA ----------
Sauer, J., Renner, U., Hopfner, U., Lange, M., Müller, A., Strasburger, C.J., Pagotto, U.,..., Stalla, G.K. (1998) . Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures. Journal of Clinical Endocrinology and Metabolism, 83(7), 2429-2434.
---------- CHICAGO ----------
Sauer, J., Renner, U., Hopfner, U., Lange, M., Müller, A., Strasburger, C.J., et al. "Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures" . Journal of Clinical Endocrinology and Metabolism 83, no. 7 (1998) : 2429-2434.
---------- MLA ----------
Sauer, J., Renner, U., Hopfner, U., Lange, M., Müller, A., Strasburger, C.J., et al. "Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures" . Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 7, 1998, pp. 2429-2434.
---------- VANCOUVER ----------
Sauer, J., Renner, U., Hopfner, U., Lange, M., Müller, A., Strasburger, C.J., et al. Interleukin-1β enhances interleukin-1 receptor antagonist content in human somatotroph adenoma cell cultures. J. Clin. Endocrinol. Metab. 1998;83(7):2429-2434.