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Abstract:

Reproducible solubilization of flourescent derivatives of glucosamine and glucose from heated silica gel adsorbent is reported. The fluorescence emission spectra of these compounds were also measured. It was found that the flourescent compounds remain stable from -20 to 30°C within a wide pH range (4-10). The presence of an adsorbent such as silica gel, paper or glass microfibre paper is essential for the generation of fluorescent derivatives. The flourescence excitability was found to be reversible under certain conditions, at least for glucosamine. © 1984.

Registro:

Documento: Artículo
Título:Solubilization and fluorescence emission spectra of substances generated by thermal treatment of carbohydrates on silica gel
Autor:Alperin, D.M.; Iusem, N.D.; Idoyaga-Vargas, V.; Carminatti, H.
Filiación:Instituto de Investigaciones Bioqumicas 'Fundacion Camponar', Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Antonio Machado 151, 1405 Buenos Aires, Argentina
Año:1984
Volumen:295
Número:C
Página de inicio:123
Página de fin:128
DOI: http://dx.doi.org/10.1016/S0021-9673(01)87603-2
Título revista:Journal of Chromatography A
Título revista abreviado:J. Chromatogr. A
ISSN:00219673
CODEN:JCRAE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v295_nC_p123_Alperin

Referencias:

  • Alperin, Idoyaga-Vargas, Carminnatti, (1982) J. Chromatogr., 242, p. 299
  • Dubra, Alperin, Sagedahl, Idoyaga-Vargas, Carminati, (1982) J. Chromatogr., 250, p. 124
  • Alperin, Carminatti, Idoyaga-Vargas, Couso, (1983) J. Chromatogr., 265, p. 193
  • Bray, (1960) Anal. Biochem., 1, p. 279
  • Shanfield, Lee, Martin, (1977) J. Chromatogr., 142, p. 387
  • Lederer, Lederer, (1954) Chromatography, , Elsevier, Amsterdam, Ch. 35
  • Segura, Gotto, Jr., (1974) J. Chromatogr., 99, p. 643
  • Zhou, Shanfield, Wang, Zlaktis, (1981) j. Chromatogr., 217, p. 341
  • Segura, Navarro, (1981) J. Chromatogr., 217, p. 329
  • Klaus, (1964) J. Chromatogr., 16, p. 311

Citas:

---------- APA ----------
Alperin, D.M., Iusem, N.D., Idoyaga-Vargas, V. & Carminatti, H. (1984) . Solubilization and fluorescence emission spectra of substances generated by thermal treatment of carbohydrates on silica gel. Journal of Chromatography A, 295(C), 123-128.
http://dx.doi.org/10.1016/S0021-9673(01)87603-2
---------- CHICAGO ----------
Alperin, D.M., Iusem, N.D., Idoyaga-Vargas, V., Carminatti, H. "Solubilization and fluorescence emission spectra of substances generated by thermal treatment of carbohydrates on silica gel" . Journal of Chromatography A 295, no. C (1984) : 123-128.
http://dx.doi.org/10.1016/S0021-9673(01)87603-2
---------- MLA ----------
Alperin, D.M., Iusem, N.D., Idoyaga-Vargas, V., Carminatti, H. "Solubilization and fluorescence emission spectra of substances generated by thermal treatment of carbohydrates on silica gel" . Journal of Chromatography A, vol. 295, no. C, 1984, pp. 123-128.
http://dx.doi.org/10.1016/S0021-9673(01)87603-2
---------- VANCOUVER ----------
Alperin, D.M., Iusem, N.D., Idoyaga-Vargas, V., Carminatti, H. Solubilization and fluorescence emission spectra of substances generated by thermal treatment of carbohydrates on silica gel. J. Chromatogr. A. 1984;295(C):123-128.
http://dx.doi.org/10.1016/S0021-9673(01)87603-2