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Abstract:

Correlation between elution volumes, Ve, and molecular weight was investigated by Bio-Gel filtration of proteins of known molecular weight on Bio-Gel P-100, P-200 and P-300 columns at pH 6.8 and 7.4. The relationship between Ve and molecular weight was similar over the pH range 4.0 to 8.0. The molecular weights of two enzymes, coproporphyrinogenase and δ-aminolaevulinic acid dehydrase, were estimated on calibrated Bio-Gel P-200 columns; their respective values were in agreement with those obtained by using calibrated Sephadex columns. The uncertainty in values estimated by this procedure was put at 12%. © 1967.

Registro:

Documento: Artículo
Título:Estimation of molecular weights of proteins by bio-gel P gel filtration
Autor:Del C. Battle, A.M.
Filiación:Catedra de Quinica Biológica, facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:protein; animal; article; cattle; gel chromatography; molecular weight; Animal; Cattle; Chromatography, Gel; Molecular Weight; Proteins
Año:1967
Volumen:28
Número:C
Página de inicio:82
Página de fin:88
Título revista:Journal of Chromatography A
Título revista abreviado:J. Chromatogr. A
ISSN:00219673
CODEN:JCRAE
CAS:protein, 67254-75-5; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle

Citas:

---------- APA ----------
(1967) . Estimation of molecular weights of proteins by bio-gel P gel filtration. Journal of Chromatography A, 28(C), 82-88.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle [ ]
---------- CHICAGO ----------
Del C. Battle, A.M. "Estimation of molecular weights of proteins by bio-gel P gel filtration" . Journal of Chromatography A 28, no. C (1967) : 82-88.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle [ ]
---------- MLA ----------
Del C. Battle, A.M. "Estimation of molecular weights of proteins by bio-gel P gel filtration" . Journal of Chromatography A, vol. 28, no. C, 1967, pp. 82-88.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle [ ]
---------- VANCOUVER ----------
Del C. Battle, A.M. Estimation of molecular weights of proteins by bio-gel P gel filtration. J. Chromatogr. A. 1967;28(C):82-88.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle [ ]