Correlation between elution volumes, Ve, and molecular weight was investigated by Bio-Gel filtration of proteins of known molecular weight on Bio-Gel P-100, P-200 and P-300 columns at pH 6.8 and 7.4. The relationship between Ve and molecular weight was similar over the pH range 4.0 to 8.0. The molecular weights of two enzymes, coproporphyrinogenase and δ-aminolaevulinic acid dehydrase, were estimated on calibrated Bio-Gel P-200 columns; their respective values were in agreement with those obtained by using calibrated Sephadex columns. The uncertainty in values estimated by this procedure was put at 12%. © 1967.
Documento: | Artículo |
Título: | Estimation of molecular weights of proteins by bio-gel P gel filtration |
Autor: | Del C. Battle, A.M. |
Filiación: | Catedra de Quinica Biológica, facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
Palabras clave: | protein; animal; article; cattle; gel chromatography; molecular weight; Animal; Cattle; Chromatography, Gel; Molecular Weight; Proteins |
Año: | 1967 |
Volumen: | 28 |
Número: | C |
Página de inicio: | 82 |
Página de fin: | 88 |
Título revista: | Journal of Chromatography A |
Título revista abreviado: | J. Chromatogr. A |
ISSN: | 00219673 |
CODEN: | JCRAE |
CAS: | protein, 67254-75-5; Proteins |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v28_nC_p82_DelCBattle |