Artículo
Abstract:
Extending work on the detection of organic compounds in thin-layer chromatography using the thermal-ultraviolet method, the differential detection of sugars based on a temperature-dependent characteristic pattern revealed under UV and visible light is reported. Specific colours related to structural changes have been observed under UV light. High recoveries of 14C- and also 3H-labelled compounds after thermal-UV detection have been obtained. The detection of lipids and the determination of the sensitivity of the method for these compounds and carbohydrates are described. Results comparable to those in sulphuric acid-based methods have been obtained. In addition, the method has the advantage of a very low background even after prolonged periods of heating at high temperatures. The detection, differentiation and recovery of several methyl glucosides is also reported. © 1983.
Registro:
| Documento: | Artículo |
| Título: | Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| Autor: | Alperin, D.M.; Carminatti, H.; Idoyaga-Vargas, V.; Couso, R.O. |
| Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina |
| Año: | 1983 |
| Volumen: | 265 |
| Número: | C |
| Página de inicio: | 193 |
| Página de fin: | 200 |
| DOI: | http://dx.doi.org/10.1016/S0021-9673(01)96716-0 |
| Título revista: | Journal of Chromatography A |
| Título revista abreviado: | J. Chromatogr. A |
| ISSN: | 00219673 |
| CODEN: | JCRAE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v265_nC_p193_Alperin |
Referencias:
- Alperin, Idoyaga-Vargas, Carminatti, (1982) J. Chromatogr., 242, p. 299
- Dubra, Alperin, Sagedahl, Idoyaga-Vargas, Carminatti, (1982) J. Chromatogr., 250, p. 124
- Folch, Lees, Sloane-Stanley, (1982) J. Biol. Chem., 226, p. 497
- Suzuki, (1976) Basic Neurochemistry, pp. 308-328. , G.J. Siegel, R.W. Albers, R. Katzman, B.W. Agranoff, Little Brown & Co, Boston
- Bazán, Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brain (1970) Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 218, p. 1
- Hakomori, (1964) J. Biochem. (Tokyo), 55, p. 205
- Tolmasky, Staneloni, Leloir, (1982) J. Biol. Chem., 257, p. 6751
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Citas:
---------- APA ----------
Alperin, D.M., Carminatti, H., Idoyaga-Vargas, V. & Couso, R.O. (1983) . Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography. Journal of Chromatography A, 265(C), 193-200.http://dx.doi.org/10.1016/S0021-9673(01)96716-0
---------- CHICAGO ----------
Alperin, D.M., Carminatti, H., Idoyaga-Vargas, V., Couso, R.O. "Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography" . Journal of Chromatography A 265, no. C (1983) : 193-200.http://dx.doi.org/10.1016/S0021-9673(01)96716-0
---------- MLA ----------
Alperin, D.M., Carminatti, H., Idoyaga-Vargas, V., Couso, R.O. "Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography" . Journal of Chromatography A, vol. 265, no. C, 1983, pp. 193-200.http://dx.doi.org/10.1016/S0021-9673(01)96716-0
---------- VANCOUVER ----------
Alperin, D.M., Carminatti, H., Idoyaga-Vargas, V., Couso, R.O. Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography. J. Chromatogr. A. 1983;265(C):193-200.http://dx.doi.org/10.1016/S0021-9673(01)96716-0
