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Abstract:

The proposed method for the detection of organic compounds on paper chromatograms with high recovery of the sample is based on the appearance of dark spots on the chromatogram after heating the paper with, for example, the hot-plate of a domestic iron. The sensitivity of the method could be increased by examinatin under UV radiation at 366mm. This extends the detection limits to the nanomole level for many substances. The application of the method gave high recoveries of radioactive glucose and lysie. With glucose it was further found that the chemical properties of the compound were preserved. The procedure is simple and can be carried out in a few seconds without reagents, using inexpensive equipment. © 1982.

Registro:

Documento: Artículo
Título:Method for the detection of organic compounds on paper chromatograms with high recovery of the sample
Autor:Alperin, D.M.; Idoyaga-Vargas, V.P.; Carminatti, H.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
Año:1982
Volumen:242
Número:2
Página de inicio:299
Página de fin:304
DOI: http://dx.doi.org/10.1016/S0021-9673(00)81704-5
Título revista:Journal of Chromatography A
Título revista abreviado:J. Chromatogr. A
ISSN:00219673
CODEN:JCRAE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v242_n2_p299_Alperin

Referencias:

  • Kraeczkowska, (1964) Ann. Univ. Mariae Curie-Sklodowska, Sect. D, 42, p. 343
  • Huggert, Nixon, (1957) Biochem. J., 1, p. 12
  • Trevelyan, Procter, Harrison, Detection of Sugars on Paper Chromatograms (1950) Nature, 165, p. 444
  • Weast, (1977) Handbook of Chemistry and Physics, , 57th ed., Chemical Rubber Co, Cleveland, OH
  • Windholz, (1976) The Merck Index, , 9th ed., Merck

Citas:

---------- APA ----------
Alperin, D.M., Idoyaga-Vargas, V.P. & Carminatti, H. (1982) . Method for the detection of organic compounds on paper chromatograms with high recovery of the sample. Journal of Chromatography A, 242(2), 299-304.
http://dx.doi.org/10.1016/S0021-9673(00)81704-5
---------- CHICAGO ----------
Alperin, D.M., Idoyaga-Vargas, V.P., Carminatti, H. "Method for the detection of organic compounds on paper chromatograms with high recovery of the sample" . Journal of Chromatography A 242, no. 2 (1982) : 299-304.
http://dx.doi.org/10.1016/S0021-9673(00)81704-5
---------- MLA ----------
Alperin, D.M., Idoyaga-Vargas, V.P., Carminatti, H. "Method for the detection of organic compounds on paper chromatograms with high recovery of the sample" . Journal of Chromatography A, vol. 242, no. 2, 1982, pp. 299-304.
http://dx.doi.org/10.1016/S0021-9673(00)81704-5
---------- VANCOUVER ----------
Alperin, D.M., Idoyaga-Vargas, V.P., Carminatti, H. Method for the detection of organic compounds on paper chromatograms with high recovery of the sample. J. Chromatogr. A. 1982;242(2):299-304.
http://dx.doi.org/10.1016/S0021-9673(00)81704-5