Abstract:
Elution diagrams obtained on affinity chromatography show that the antigenic fractions of human seminal plasma studied, namely (a) the non-dialyzable components of human seminal plasma and (b) its trichloroacetic acid-soluble fraction, (c) the tri-chloroacetic acid-soluble fraction of whole human seminal plasma and (d) the pronase digested human seminal plasma, are complex mixtures of glycoproteins with minor amounts of polysaccharides. Some of these glycoproteins contain significant percentages of carbohydrates while others contain only trace amounts. Most of the glycoproteins carry non-reducing end-chain groups comprising a-d-glucopyranosyl, a-d-mannopyranosyl or sterically related residues. © 1979.
Registro:
Documento: |
Artículo
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Título: | Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma |
Autor: | Mazzini, M.N.; Cerezo, A.S.; De Cerezo, J.M.S. |
Filiación: | Departamento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Pabellón 2, Ciudad Universitaria, Buenos Aires Argentina Catedra de Biologia, Facultad de Farmacia y Bioquimica, Buenos Aires, Centro de Investigaciones en Reproducción, Piso 10, Buenos Aires, Argentina
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Palabras clave: | concanavalin a; sperm antigen; heredity; human cell; male genital system; methodology; normal human; Antigens; Chromatography, Affinity; Concanavalin A; Glycoproteins; Human; Male; Polysaccharides; Pronase; Semen; Sepharose; Trichloroacetic Acid |
Año: | 1979
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Volumen: | 173
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Número: | 2
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Página de inicio: | 365
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Página de fin: | 371
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DOI: |
http://dx.doi.org/10.1016/S0021-9673(00)92305-7 |
Título revista: | Journal of Chromatography A
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Título revista abreviado: | J. Chromatogr. A
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ISSN: | 00219673
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CODEN: | JCRAE
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CAS: | concanavalin A, 11028-71-0; Antigens; Concanavalin A, 11028-71-0; Glycoproteins; Polysaccharides; Pronase, EC 3.4.24.-; Sepharose, 9012-36-6; Trichloroacetic Acid, 76-03-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219673_v173_n2_p365_Mazzini |
Referencias:
- Goldstein, So, (1965) Arch. Biochem. Biophys., 111, p. 405
- Kennedy, Rosevear, An assessment of the fractionation of carbohydrates on Concanavalin A?Sepharose 4B by affinity chromatography (1973) Journal of the Chemical Society, Perkin Transactions 1, p. 2041
- Cerezo, (1974) Int. J. Fertil., 19, p. 211
- Cerezo, Cerezo, (1977) Int. J. Fertil., 22, p. 162
- J.M.S. Cerezo and A.S. Cerezo, unpublished results; Aspberg, Porath, Group-specific Adsorption of Glycoproteins. (1970) Acta Chemica Scandinavica, 24, p. 1893
- Dubois, Gilles, Hamilton, Rebers, Smith, (1956) Anal. Chem., 28, p. 350
- Warburg, Christian, (1957) Methods Enzymol., 111, p. 451
- Leschziner, Cereno, (1970) Carbohyd. Res., 15, p. 291
- Montgomery, (1970) The Carbohydrates, Chemistry and Biochemistry, 11 B, p. 647. , W. Pigman, D. Horton, Academic Press, New York
- Shulman, Bronson, (1968) Fertil. Steril., 19, p. 549
- Shulman, Bronson, (1969) J. Reprod. Fertil., 18, p. 481
- Li, Beling, (1973) Fertil. Steril., 24, p. 134
- Shulman, Li, (1974) Int. J. Fertil., 19, p. 19
- Li, Shulman, (1971) Int. J. Fertil., 16, p. 87
- Herrman, Herrman, (1969) Fertil. Steril., 20, p. 521
- Katsh, (1961) Int. J. Fertil., 6, p. 53
- Hekman, Runkel, (1969) Fertil. Steril., 20, p. 312
- Defagio, Lewis, Ketchel, ANTIGENIC VARIATIONS IN THE SEMINAL PLASMA OF DIFFERENT MEN (1969) Reproduction, 20, p. 29
- Herrman, Immunologische Charakterisierung menschlicher Spermaplasmaproteine/Immunological characterization of human seminal plasma proteins (1969) Andrologia, 1, p. 11
- Goldstein, Iyer, (1966) Biochim. Biophys. Acta, 121, p. 197
Citas:
---------- APA ----------
Mazzini, M.N., Cerezo, A.S. & De Cerezo, J.M.S.
(1979)
. Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma. Journal of Chromatography A, 173(2), 365-371.
http://dx.doi.org/10.1016/S0021-9673(00)92305-7---------- CHICAGO ----------
Mazzini, M.N., Cerezo, A.S., De Cerezo, J.M.S.
"Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma"
. Journal of Chromatography A 173, no. 2
(1979) : 365-371.
http://dx.doi.org/10.1016/S0021-9673(00)92305-7---------- MLA ----------
Mazzini, M.N., Cerezo, A.S., De Cerezo, J.M.S.
"Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma"
. Journal of Chromatography A, vol. 173, no. 2, 1979, pp. 365-371.
http://dx.doi.org/10.1016/S0021-9673(00)92305-7---------- VANCOUVER ----------
Mazzini, M.N., Cerezo, A.S., De Cerezo, J.M.S. Affinity chromatography on concanavalin a-sepharose of antigenic fractions of human seminal plasma. J. Chromatogr. A. 1979;173(2):365-371.
http://dx.doi.org/10.1016/S0021-9673(00)92305-7