Amiano, N.O.; Costa, M.J.; Reiteri, R.M.; Payés, C.; Guerrieri, D.; Tateosian, N.L.; Sánchez, M.L.; Maffia, P.C.; Diament, M.; Karas, R.; Orqueda, A.; Rizzo, M.; Alaniz, L.; Mazzolini, G.; Klein, S.; Sallenave, J.-M.; Chuluyan, H.E. "Anti-tumor effect of SLPI on mammary but not colon tumor growth" (2013) Journal of Cellular Physiology. 228(2):469-475
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Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasis dissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this study was to examine the effect of SLPI on mammary and colon tumor growth. The effect of SLPI was tested on in vitro cell apoptosis and in vivo tumor growth experiments. SLPI over-expressing human and murine mammary and colon tumor cells were generated by gene transfection. The administration of murine mammary tumor cells over-expressing high levels of SLPI did not develop tumors in mice. On the contrary, the administration of murine colon tumor cells over-expressing SLPI, developed faster tumors than control cells. Intratumoral, but not intraperitoneal administration of SLPI, delayed the growth of tumors and increased the survival of mammary but not colon tumor bearing mice. In vitro culture of mammary tumor cell lines treated with SLPI, and SLPI producer clones were more prone to apoptosis than control cells, mainly under serum deprivation culture conditions. Herein we demonstrated that SLPI induces the apoptosis of mammary tumor cells in vitro and decreases the mammary but not colon tumor growth in vivo. Therefore, SLPI may be a new potential therapeutic tool for certain tumors, such as mammary tumors. © 2012 Wiley Periodicals, Inc.


Documento: Artículo
Título:Anti-tumor effect of SLPI on mammary but not colon tumor growth
Autor:Amiano, N.O.; Costa, M.J.; Reiteri, R.M.; Payés, C.; Guerrieri, D.; Tateosian, N.L.; Sánchez, M.L.; Maffia, P.C.; Diament, M.; Karas, R.; Orqueda, A.; Rizzo, M.; Alaniz, L.; Mazzolini, G.; Klein, S.; Sallenave, J.-M.; Chuluyan, H.E.
Filiación:Department of Pharmacology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina
Animal Care and Experimental Cancer Research, Oncology Institute A.H. Roffo, Buenos Aires, Argentina
Department of Biological Chemistry, FCEN, IFIBYNE - UBA - CONICET, Buenos Aires, Argentina
Gene Therapy Laboratory, School of Biomedical Science, Austral University, Pilar, Prov. Buenos Aires, Argentina
Unité de Défense Innée et Inflammation, Institut Pasteur, Paris, France
Institut National de la Santé et de la Recherche Médicale (INSERM) U874, Paris, France
Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Rue du Dr Roux, Paris, France
Palabras clave:caspase 3; secretory leukocyte proteinase inhibitor; small interfering RNA; animal cell; animal experiment; animal model; antineoplastic activity; apoptosis; article; breast adenocarcinoma; breast cancer; cancer chemotherapy; cancer survival; cell proliferation; cell strain MCF 7; cell structure; colon cancer; controlled study; enzyme activity; enzyme linked immunosorbent assay; female; flow cytometry; genetic transfection; HeLa cell; human; human cell; in vitro study; in vivo study; lung metastasis; mouse; nonhuman; priority journal; protein expression; protein purification; tumor growth; Animals; Apoptosis; Breast Neoplasms; Cell Line, Tumor; Colonic Neoplasms; Colorectal Neoplasms; Female; Gene Silencing; Humans; Lung Neoplasms; Mammary Neoplasms, Animal; Mice; Mice, Inbred BALB C; Secretory Leukocyte Peptidase Inhibitor; Transfection; Uterine Cervical Neoplasms; Murinae; Mus
Página de inicio:469
Página de fin:475
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
CAS:caspase 3, 169592-56-7; Secretory Leukocyte Peptidase Inhibitor


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---------- APA ----------
Amiano, N.O., Costa, M.J., Reiteri, R.M., Payés, C., Guerrieri, D., Tateosian, N.L., Sánchez, M.L.,..., Chuluyan, H.E. (2013) . Anti-tumor effect of SLPI on mammary but not colon tumor growth. Journal of Cellular Physiology, 228(2), 469-475.
---------- CHICAGO ----------
Amiano, N.O., Costa, M.J., Reiteri, R.M., Payés, C., Guerrieri, D., Tateosian, N.L., et al. "Anti-tumor effect of SLPI on mammary but not colon tumor growth" . Journal of Cellular Physiology 228, no. 2 (2013) : 469-475.
---------- MLA ----------
Amiano, N.O., Costa, M.J., Reiteri, R.M., Payés, C., Guerrieri, D., Tateosian, N.L., et al. "Anti-tumor effect of SLPI on mammary but not colon tumor growth" . Journal of Cellular Physiology, vol. 228, no. 2, 2013, pp. 469-475.
---------- VANCOUVER ----------
Amiano, N.O., Costa, M.J., Reiteri, R.M., Payés, C., Guerrieri, D., Tateosian, N.L., et al. Anti-tumor effect of SLPI on mammary but not colon tumor growth. J. Cell. Physiol. 2013;228(2):469-475.