Artículo

Vallejo, G.; Maschi, D.; Mestre-Citrinovitz, A.C.; Aiba, K.; Maronna, R.; Yohai, V.; Ko, M.S.H.; Beato, M.; Saragüeta, P. "Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells" (2010) Journal of Cellular Physiology. 222(1):127-137
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Abstract:

During the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3-fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells. © 2009 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
Autor:Vallejo, G.; Maschi, D.; Mestre-Citrinovitz, A.C.; Aiba, K.; Maronna, R.; Yohai, V.; Ko, M.S.H.; Beato, M.; Saragüeta, P.
Filiación:Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Obligado 2490, 1428 Buenos Aires, Argentina
Laboratory of Genetics, National Institute on Aging, Baltimore, MD, United States
Departamento de Matemática, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, La Plata, Argentina
Departamento de Matemática, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
CICPBA, La Plata, Argentina
Centre de Regulació Genòmica, Universidad Pompeu Fabra, Barcelona, Spain
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:complementary DNA; matrix protein; procollagen C proteinase; structural protein; WT1 protein; amino terminal sequence of split gene; animal experiment; article; cell culture; cell proliferation; cell structure; chromatin; cold shock domain containing c2 gene; cytoarchitecture; decidualization; DNA microarray; eef1a1 gene; endometrium cell; female; gene; gene expression; gene identification; guanine nucleotide binding protein alpha 12 gene; in vitro study; multiple endocrine neoplasia 1 gene; nonhuman; pregnancy; priority journal; protein synthesis; rat; serum; signal transduction; tripartite motif containing 27 gene; wilms tumor 1 gene; wt1 gene; Animals; Cell Differentiation; Decidua; Down-Regulation; Female; Gene Expression Profiling; Gene Expression Regulation, Developmental; Oligonucleotide Array Sequence Analysis; Pregnancy; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Stromal Cells; Up-Regulation; Rattus
Año:2010
Volumen:222
Número:1
Página de inicio:127
Página de fin:137
DOI: http://dx.doi.org/10.1002/jcp.21929
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
ISSN:00219541
CODEN:JCLLA
CAS:procollagen C proteinase, 68651-95-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v222_n1_p127_Vallejo

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Citas:

---------- APA ----------
Vallejo, G., Maschi, D., Mestre-Citrinovitz, A.C., Aiba, K., Maronna, R., Yohai, V., Ko, M.S.H.,..., Saragüeta, P. (2010) . Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells. Journal of Cellular Physiology, 222(1), 127-137.
http://dx.doi.org/10.1002/jcp.21929
---------- CHICAGO ----------
Vallejo, G., Maschi, D., Mestre-Citrinovitz, A.C., Aiba, K., Maronna, R., Yohai, V., et al. "Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells" . Journal of Cellular Physiology 222, no. 1 (2010) : 127-137.
http://dx.doi.org/10.1002/jcp.21929
---------- MLA ----------
Vallejo, G., Maschi, D., Mestre-Citrinovitz, A.C., Aiba, K., Maronna, R., Yohai, V., et al. "Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells" . Journal of Cellular Physiology, vol. 222, no. 1, 2010, pp. 127-137.
http://dx.doi.org/10.1002/jcp.21929
---------- VANCOUVER ----------
Vallejo, G., Maschi, D., Mestre-Citrinovitz, A.C., Aiba, K., Maronna, R., Yohai, V., et al. Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells. J. Cell. Physiol. 2010;222(1):127-137.
http://dx.doi.org/10.1002/jcp.21929