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Gironella, M.; Malicet, C.; Cano, C.; Sandi, M.J.; Hamidi, T.; Tauil, R.M.N.; Baston, M.; Valaco, P.; Moreno, S.; Lopez, F.; Neira, J.L.; Dagorn, J.C.; Iovanna, J.L. "p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage" (2009) Journal of Cellular Physiology. 221(3):594-602
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Abstract:

The stress protein p8 is a small, highly basic, unfolded, and multifunctional protein.Wehave previously shown that most of its functions are exerted through interactions with other proteins, whose activities are thereby enhanced or repressed. In this work we describe another example of such mechanism, by which p8 binds and negatively regulates MSL1, a histone acetyl transferase (HAT)-associated protein, which in turn binds the DNA-damage-associated 53BP1 protein to facilitate DNA repair following DNA γ-irradiation. Contrary to the HAT-associated activity, MSL1-dependent DNA-repair activity is almost completely dependent on 53BP1 expression. The picture that has emerged from our findings is that 53BP1 could be a scaffold that gets the HAT MSL1-dependent DNA-repair activity to the sites of DNA damage. Finally, we also found that, although p8 expression is transiently activated after γ-irradiation, it is eventually submitted to sustained down-regulation, presumably to allow development of MSL1-associated DNA-repair activity. Weconclude that interaction of MSL1 with 53BP1 brings MSL1-dependent HAT activity to the vicinity of damaged DNA. MSL1-dependent HAT activity, which is negatively regulated by the stress protein p8, induces chromatin remodeling and relaxation allowing access to DNA of the repair machinery. © 2009 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage
Autor:Gironella, M.; Malicet, C.; Cano, C.; Sandi, M.J.; Hamidi, T.; Tauil, R.M.N.; Baston, M.; Valaco, P.; Moreno, S.; Lopez, F.; Neira, J.L.; Dagorn, J.C.; Iovanna, J.L.
Filiación:INSERM U.624, Stress Cellulaire, Parc Scientifique et Technologique de Luminy, Case 915, Marseille Cedex 9, France
Centro de Investigacion Biomedica en Red de Enfermedades Hepaticas Y Digestivas (Ciberehd), Barcelona, Spain
Departamento de Química Biológica, Ciudad Universitaria, Buenos Aires, Argentina
INSERM IFR31, Institut Louis Bugnard, Hôpital Rangueil, Toulouse Cedex 4, France
Instituto de Biología Molecular Y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain
Biocomputation and Complex Systems Physics Institute, Zaragoza, Spain
Palabras clave:double stranded DNA; histone acetyltransferase; protein p8; basic helix loop helix transcription factor; histone; histone acetyltransferase; MSL1 protein, human; nuclear protein; P8 protein, human; recombinant protein; signal peptide; small interfering RNA; TP53BP1 protein, human; tumor protein; article; chromatin; controlled study; DNA damage; DNA repair; down regulation; female; gamma irradiation; human; human cell; priority journal; protein binding; protein expression; protein protein interaction; cell line; cell proliferation; chemistry; clonogenic assay; double stranded DNA break; gamma radiation; gene expression; genetic transfection; genetics; HeLa cell; immunoprecipitation; metabolism; physiology; radiation exposure; surface plasmon resonance; two hybrid system; Basic Helix-Loop-Helix Transcription Factors; Cell Line; Cell Proliferation; Colony-Forming Units Assay; DNA Breaks, Double-Stranded; DNA Repair; Gamma Rays; Gene Expression; Hela Cells; Histone Acetyltransferases; Histones; Humans; Immunoprecipitation; Intracellular Signaling Peptides and Proteins; Neoplasm Proteins; Nuclear Proteins; Protein Binding; Recombinant Proteins; RNA, Small Interfering; Surface Plasmon Resonance; Transfection; Two-Hybrid System Techniques
Año:2009
Volumen:221
Número:3
Página de inicio:594
Página de fin:602
DOI: http://dx.doi.org/10.1002/jcp.21889
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
ISSN:00219541
CODEN:JCLLA
CAS:histone acetyltransferase, 9054-51-7; histone acetyltransferase KAT5; histone, 9062-68-4; histone acetyltransferase, 9054-51-7; Basic Helix-Loop-Helix Transcription Factors; Histone Acetyltransferases, 2.3.1.48; Histones; Intracellular Signaling Peptides and Proteins; MSL1 protein, human, 2.3.1.48; Neoplasm Proteins; Nuclear Proteins; P8 protein, human; RNA, Small Interfering; Recombinant Proteins; TP53BP1 protein, human
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v221_n3_p594_Gironella

Referencias:

  • Bird, A.W., Yu, D.Y., Pray-Grant, M.G., Qiu, Q., Harmon, K.E., Megee, P.C., Grant, P.A., Christman, M.F., Acetylation of histone H4 by Esa1 is required forDNAdouble-strand break repair (2002) Nature, 419, pp. 411-415
  • Bousquet, C., Guillermet-Guibert, J., Saint-Laurent, N., Archer-Lahlou, E., Lopez, F., Fanjul, M., Ferrand, A., Susini, C., Direct binding of p85 to sst2 somatostatin receptor reveals a novel mechanism for inhibiting PI3K pathway (2006) EMBO Journal, 25 (17), pp. 3943-3954. , DOI 10.1038/sj.emboj.7601279, PII 7601279
  • Carracedo, A., Gironella, M., Lorente, M., Garcia, S., Guzman, M., Velasco, G., Iovanna, J.L., Cannabinoids induce apoptosis of pancreatic tumor cells via endoplasmic reticulum stress-related genes (2006) Cancer Research, 66 (13), pp. 6748-6755. , DOI 10.1158/0008-5472.CAN-06-0169
  • Carracedo, A., Lorente, M., Egia, A., Blazquez, C., Garcia, S., Giroux, V., Malicet, C., Velasco, G., The stress-regulated protein p8 mediates cannabinoid-induced apoptosis of tumor cells (2006) Cancer Cell, 9, pp. 301-312
  • Carrozza, M.J., Utley, R.T., Workman, J.L., Cote, J., The diverse functions of histone acetyltransferase complexes (2003) Trends in Genetics, 19 (6), pp. 321-329. , DOI 10.1016/S0168-9525(03)00115-X
  • Encinar, J.A., Mallo, G.V., Mizyrycki, C., Giono, L., Gonzalez-Ros, J.M., Rico, M., Canepa, E., Iovanna, J.L., Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation (2001) Journal of Biological Chemistry, 276 (4), pp. 2742-2751. , DOI 10.1074/jbc.M008594200
  • Garcia-Montero, A., Vasseur, S., Mallo, G.V., Soubeyran, P., Dagorn, J.C., Iovanna, J.L., Expression of the stress-induced p8 mRNA is transiently activated after culture medium change (2001) European Journal of Cell Biology, 80 (11), pp. 720-725
  • Garcia-Montero, A.C., Vasseur, S., Giono, L.E., Canepa, E., Moreno, S., Dagorn, J.C., Iovanna, J.L., Transforming growth factor beta-1 enhances Smad transcriptional activity through activation of p8 gene expression (2001) Biochemical Journal, 357 (1), pp. 249-253. , DOI 10.1042/0264-6021:3570249
  • Goruppi, S., Kyriakis, J.M., The pro-hypertrophic basic helix-loop-helix protein p8 is degraded by the ubiquitin/proteasome system in a protein kinase B/Akt- And glycogen synthase kinase-3-dependent manner, whereas endothelin induction of p8 mRNA and renal mesangial cell hypertrophy require NFAT4 (2004) Journal of Biological Chemistry, 279 (20), pp. 20950-20958. , DOI 10.1074/jbc.M312401200
  • Goruppi, S., Bonventre, J.V., Kyriakis, J.M., Signaling pathways and late-onset gene induction associated with renal mesangial cell hypertrophy (2002) EMBO Journal, 21 (20), pp. 5427-5436. , DOI 10.1093/emboj/cdf535
  • Goruppi, S., Patten, R.D., Force, T., Kyriakis, J.M., Helix-loop-helix protein p8, a transcriptional regulator required for cardiomyocyte hypertrophy and cardiac fibroblast matrix metalloprotease induction (2007) Molecular and Cellular Biology, 27 (3), pp. 993-1006. , DOI 10.1128/MCB.00996-06
  • Gupta, A., Sharma, G.G., Young, C.S.H., Agarwal, M., Smith, E.R., Paull, T.T., Lucchesi, J.C., Pandita, T.K., Involvement of human MOF in ATM function (2005) Molecular and Cellular Biology, 25 (12), pp. 5292-5305. , DOI 10.1128/MCB.25.12.5292-5305.2005
  • Hoffmeister, A., Ropolo, A., Vasseur, S., Mallo, G.V., Bodeker, H., Ritz-Laser, B., Dressler, G.R., Iovanna, J.L., The HMG-I/Y-related protein p8 binds to p300 and Pax2 trans-activation domain-interacting protein to regulate the trans-activation activity of the Pax2A and Pax2B transcription factors on the glucagon gene promoter (2002) J Biol Chem, 277, pp. 22314-22319
  • Igarashi, T., Kuroda, H., Takahashi, S., Asashima, M., Cloning and characterization of the Xenopus laevis p8 gene (2001) Development Growth and Differentiation, 43 (6), pp. 693-698. , DOI 10.1046/j.1440-169X.2001.00613.x
  • Ito, Y., Yoshida, H., Motoo, Y., Miyoshi, E., Iovanna, J.L., Tomoda, C., Uruno, T., Miyauchi, A., Expression and cellular localization of p8 protein in thyroid neoplasms (2003) Cancer Letters, 201 (2), pp. 237-244. , DOI 10.1016/j.canlet.2003.07.002
  • Ito, Y., Yoshida, H., Motoo, Y., Iovanna, J.L., Nakamura, Y., Kakudo, K., Uruno, T., Miyauchi, A., Expression of P8 protein in breast carcinoma; an inverse relationship with apoptosis (2005) Anticancer Research, 25 (2 A), pp. 833-837
  • Ito, Y., Yoshida, H., Motoo, Y., Iovanna, J.L., Tomoda, C., Uruno, T., Takamura, Y., Miyauchi, A., Expression of p8 protein in medullary thyroid carcinoma (2005) Anticancer Research, 25 (5), pp. 3419-3423
  • Iwabuchi, K., Basu, B.P., Kysela, B., Kurihara, T., Shibata, M., Guan, D., Cao, Y., Doherty, A.J., Potential role for 53BP1 in DNA end-joining repair through direct interaction with DNA (2003) Journal of Biological Chemistry, 278 (38), pp. 36487-36495. , DOI 10.1074/jbc.M304066200
  • Jiang, Y.-F., Vaccaro, M.I., Fiedler, F., Calvo, E.L., Iovanna, J.L., Lipopolysaccharides induce p8 mRNA expression in vivo and in vitro (1999) Biochemical and Biophysical Research Communications, 260 (3), pp. 686-690. , DOI 10.1006/bbrc.1999.0953
  • Kallwellis, K., Grempler, R., Gunther, S., Path, G., Walther, R., Tumor necrosis factor alpha induces the expression of the nuclear protein p8 via a novel NFkappaB binding site within the promoter (2006) Hormone and Metabolic Research, 38 (9), pp. 570-574. , DOI 10.1055/s-2006-950503
  • Malicet, C., Hoffmeister, A., Moreno, S., Closa, D., Dagorn, J.-C., Vasseur, S., Iovanna, J.L., Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation (2006) Biochemical and Biophysical Research Communications, 339 (1), pp. 284-289. , DOI 10.1016/j.bbrc.2005.11.018, PII S0006291X05025301
  • Malicet, C., Dagorn, J.C., Neira, J.L., Iovanna, J.L., P8 and prothymosin alpha: Unity is strength (2006) Cell Cycle, 5, pp. 829-830
  • Malicet, C., Giroux, V., Vasseur, S., Dagorn, J.C., Neira, J.L., Iovanna, J.L., Regulation of apoptosis by the p8/prothymosin alpha complex (2006) Proc Natl Acad Sci USA, 103, pp. 2671-2676
  • Mallo, G.V., Fiedler, F., Calvo, E.L., Ortiz, E.M., Vasseur, S., Keim, V., Morisset, J., Iovanna, J.L., Cloning and expression of the rat p8 cDNA, a new gene activated in pancreas during the acute phase of pancreatitis, pancreatic development, and regeneration, and which promotes cellular growth (1997) Journal of Biological Chemistry, 272 (51), pp. 32360-32369. , DOI 10.1074/jbc.272.51.32360
  • Mochan, T.A., Venere, M., Ditullio Jr., R.A., Halazonetis, T.D., 53BP1, an activator of ATM in response to DNA damage (2004) DNA Repair, 3 (8-9), pp. 945-952. , DOI 10.1016/j.dnarep.2004.03.017, PII S156878640400076X
  • Mohammad, H.P., Seachrist, D.D., Quirk, C.C., Nilson, J.H., Reexpression of p8 contributes to tumorigenic properties of pituitary cells and appears in a subset of prolactinomas in transgenic mice that hypersecrete luteinizing hormone (2004) Mol Endocrinol, 18, pp. 2583-2593
  • Murr, R., Loizou, J.I., Yang, Y.-G., Cuenin, C., Li, H., Wang, Z.-Q., Herceg, Z., Histone acetylation by Trrap-Tip60 modulates loading of repair proteins and repair of DNA double-strand breaks (2006) Nature Cell Biology, 8 (1), pp. 91-99. , DOI 10.1038/ncb1343, PII N1343
  • Path, G., Opel, A., Gehlen, M., Rothhammer, V., Niu, X., Limbert, C., Romfeld, L., Seufert, J., Glucose-dependent expansion of pancreatic beta-cells by the protein p8 in vitro and in vivo (2006) American Journal of Physiology - Endocrinology and Metabolism, 291 (6), pp. E1168-E1176. , http://ajpendo.physiology.org/cgi/reprint/291/6/E1168, DOI 10.1152/ajpendo.00436.2005
  • Peterson, C.L., Cote, J., Cellular machineries for chromosomal DNA repair (2004) Genes and Development, 18 (6), pp. 602-616. , DOI 10.1101/gad.1182704
  • Plant, S.R., Wang, Y., Vasseur, S., Thrash, J.C., McMahon, E.J., Bergstralh, D.T., Arnett, H.A., Ting, J.P., Upregulation of the stress-associated gene p8 in mouse models of demyelination and in multiple sclerosis tissues (2006) Glia, 53, pp. 529-537
  • Quirk, C.C., Seachrist, D.D., Nilson, J.H., Embryonic expression of the luteinizing hormone beta gene appears to be coupled to the transient appearance of p8, a high mobility group-related transcription factor (2003) J Biol Chem, 278, pp. 1680-1685
  • Rea, S., Xouri, G., Akhtar, A., Males absent on the first (MOF): From flies to humans (2007) Oncogene, 26 (37), pp. 5385-5394. , DOI 10.1038/sj.onc.1210607, PII 1210607
  • Sass, G.L., Pannuti, A., Lucchesi, J.C., Male-specific lethal complex of Drosophila targets activated regions of the X chromosome for chromatin remodeling (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (14), pp. 8287-8291. , DOI 10.1073/pnas.1332749100
  • Shogren-Knaak, M., Ishii, H., Sun, J.-M., Pazin, M.J., Davie, J.R., Peterson, C.L., Histone H4-K16 acetylation controls chromatin structure and protein interactions (2006) Science, 311 (5762), pp. 844-847. , DOI 10.1126/science.1124000
  • Smith, E.R., Allis, C.D., Lucchesi, J.C., Linking Global Histone Acetylation to the Transcription Enhancement of X-chromosomal Genes in Drosophila Males (2001) Journal of Biological Chemistry, 276 (34), pp. 31483-31486. , DOI 10.1074/jbc.C100351200
  • Smith, E.R., Cayrou, C., Huang, R., Lane, W.S., Cote, J., Lucchesi, J.C., A human protein complex homologous to the Drosophila MSL complex is responsible for the majority of histone H4 acetylation at lysine 16 (2005) Molecular and Cellular Biology, 25 (21), pp. 9175-9188. , DOI 10.1128/MCB.25.21.9175-9188.2005
  • Su, S.B., Motoo, Y., Iovanna, J.L., Xie, M.J., Mouri, H., Ohtsubo, K., Yamaguchi, Y., Sawabu, N., Expression of p8 in human pancreatic cancer (2001) Clin Cancer Res, 7, pp. 309-313
  • Sun, Y., Jiang, X., Chen, S., Price, B.D., Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation (2006) FEBS Letters, 580 (18), pp. 4353-4356. , DOI 10.1016/j.febslet.2006.06.092, PII S0014579306008167
  • Sykes, S.M., Mellert, H.S., Holbert, M.A., Li, K., Marmorstein, R., Lane, W.S., McMahon, S.B., Acetylation of the p53 DNA-Binding Domain Regulates Apoptosis Induction (2006) Molecular Cell, 24 (6), pp. 841-851. , DOI 10.1016/j.molcel.2006.11.026, PII S1097276506008215
  • Taieb, D., Malicet, C., Garcia, S., Rocchi, P., Arnaud, C., Dagorn, J.-C., Iovanna, J.L., Vasseur, S., Inactivation of stress protein p8 increases murine carbon tetrachloride hepatotoxicity via preserved CYP2E1 activity (2005) Hepatology, 42 (1), pp. 176-182. , DOI 10.1002/hep.20759
  • Utley, R.T., Lacoste, N., Jobin-Robitaille, O., Allard, S., Cote, J., Regulation of NuA4 histone acetyltransferase activity in transcription and DNA repair by phosphorylation of histone H4 (2005) Molecular and Cellular Biology, 25 (18), pp. 8179-8190. , DOI 10.1128/MCB.25.18.8179-8190.2005
  • Valacco, M.P., Varone, C., Malicet, C., Canepa, E., Iovanna, J.L., Moreno, S., Cell growth-dependent subcellular localization of p8 (2006) J Cell Biochem, 97, pp. 1066-1079
  • Vasseur, S., Mallo, G.V., Fiedler, F., Bodeker, H., Canepa, E., Moreno, S., Iovanna, J.L., Cloning and expression of the human p8, a nuclear protein with mitogenic activity (1999) European Journal of Biochemistry, 259 (3), pp. 670-675. , DOI 10.1046/j.1432-1327.1999.00092.x
  • Vasseur, S., Hoffmeister, A., Garcia, S., Bagnis, C., Dagorn, J.-C., Iovanna, J.L., P8 is critical for tumour development induced by rasV12 mutated protein and E1A oncogene (2002) EMBO Reports, 3 (2), pp. 165-170. , DOI 10.1093/embo-reports/kvf023
  • Vasseur, S., Hoffmeister, A., Garcia-Montero, A., Barthet, M., Saint-Michel, L., Berthezene, P., Fiedler, F., Iovanna, J.L., Mice with targeted disruption of p8 gene show increased sensitivity to lipopolysaccharide and DNA microarray analysis of livers reveals an aberrant gene expression response (2003) BMC Gastroenterol, 3, p. 25
  • Wang, B., Matsuoka, S., Carpenter, P.B., Elledge, S.J., 53BP1, a mediator of the DNA damage checkpoint (2002) Science, 298, pp. 1435-1438
  • Zinke, I., Schutz, C.S., Katzenberger, J.D., Bauer, M., Pankratz, M.J., Nutrient control of gene expression in Drosophila: Microarray analysis of starvation and sugar-dependent response (2002) EMBO Journal, 21 (22), pp. 6162-6173. , DOI 10.1093/emboj/cdf600

Citas:

---------- APA ----------
Gironella, M., Malicet, C., Cano, C., Sandi, M.J., Hamidi, T., Tauil, R.M.N., Baston, M.,..., Iovanna, J.L. (2009) . p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage. Journal of Cellular Physiology, 221(3), 594-602.
http://dx.doi.org/10.1002/jcp.21889
---------- CHICAGO ----------
Gironella, M., Malicet, C., Cano, C., Sandi, M.J., Hamidi, T., Tauil, R.M.N., et al. "p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage" . Journal of Cellular Physiology 221, no. 3 (2009) : 594-602.
http://dx.doi.org/10.1002/jcp.21889
---------- MLA ----------
Gironella, M., Malicet, C., Cano, C., Sandi, M.J., Hamidi, T., Tauil, R.M.N., et al. "p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage" . Journal of Cellular Physiology, vol. 221, no. 3, 2009, pp. 594-602.
http://dx.doi.org/10.1002/jcp.21889
---------- VANCOUVER ----------
Gironella, M., Malicet, C., Cano, C., Sandi, M.J., Hamidi, T., Tauil, R.M.N., et al. p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage. J. Cell. Physiol. 2009;221(3):594-602.
http://dx.doi.org/10.1002/jcp.21889