Artículo

Fazzini, M.; Vallejo, G.; Colman-Lerner, A.; Trigo, R.; Campo, S.; Barañao, J.L.S.; Saragüeta, P.E. "Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation" (2006) Journal of Cellular Physiology. 207(1):40-48
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In order to test the hypothesis that transforming growth factor β (TGF-β) acts by FS regulation on bovine granulosa cells in in vitro differentiation, we analyzed the effect of TGF-β1 on follistatin mRNA expression in three differentiation states of bovine granulosa cells. We showed a positive regulation of FS mRNA after TGF-β1 (1 ng/ml) treatment of freshly isolated granulosa cells from small-medium antral follicles (2-8 mm). This effect was abolished by the addition of exogenous follistatin (100 ng/ml), suggesting that this effect could be mediated by activin. Although these cells showed a similar effect on FS mRNA expression after treatment with activin-A, a soluble form of activin receptor type IIA was unable to inactivate the TGF-β effect. When we tested the TGF-β effect on FS mRNA in different granulosa cell states, TGF-β1 regulation was associated with progesterone production only in freshly isolated cells. The amount of total activin-A produced by first passage cells (dedifferentiated cells), was ten times smaller than the one measured in a conditioned medium from freshly isolated cells (mature cells). The TGF-β1-dependent FS mRNA expression persisted in first passage cells without changes with FS addition. On the other hand, the BGC-1 granulosa cell line (immature cells) produced large amounts of activin-A regulated by TGF-β1 and an invariable steady state of FS mRNAs. In summary, our results showed that FS mRNA expression is regulated by TGF-β1 independently of activin effects in differentiated granulosa cells. © 2005 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation
Autor:Fazzini, M.; Vallejo, G.; Colman-Lerner, A.; Trigo, R.; Campo, S.; Barañao, J.L.S.; Saragüeta, P.E.
Filiación:Instituto de Biologia y Medicina Experimental-CONICET, Dto. de Quimica Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Investigaciones Endocrinológicas, Hospital de Niños Ricardo Gutierrez, Buenos Aires, Argentina
Molecular Sciences Institute, Berkeley, CA 94704, United States
Vta. de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:activin; activin A; activin receptor 2; activin receptor 2A; follistatin; messenger RNA; progesterone; transforming growth factor beta; transforming growth factor beta1; unclassified drug; animal cell; article; cell differentiation; cell maturation; cell size; controlled study; cow; granulosa cell; in vitro study; nonhuman; ovary follicle; priority journal; progesterone synthesis; steady state; Activin Receptors, Type II; Activins; Animals; Binding, Competitive; Cattle; Cell Differentiation; Cell Line; Cells, Cultured; Culture Media, Conditioned; Female; Fibronectins; Follistatin; Gene Expression; Granulosa Cells; Humans; Inhibin-beta Subunits; Progesterone; Receptors, Transforming Growth Factor beta; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Transforming Growth Factor beta; Transforming Growth Factor beta1; Animalia; Bovinae
Año:2006
Volumen:207
Número:1
Página de inicio:40
Página de fin:48
DOI: http://dx.doi.org/10.1002/jcp.20533
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
ISSN:00219541
CODEN:JCLLA
CAS:activin A, 104625-48-1; activin, 114949-22-3; follistatin, 117628-82-7; progesterone, 57-83-0; activin A; activin receptor type II-A, EC 2.7.1.-; Activin Receptors, Type II, EC 2.7.1.37; Activins, 104625-48-1; Culture Media, Conditioned; Fibronectins; Follistatin; Inhibin-beta Subunits, 93443-12-0; Progesterone, 57-83-0; Receptors, Transforming Growth Factor beta; RNA, Messenger; TGFB1 protein, human; Transforming Growth Factor beta; Transforming Growth Factor beta1; transforming growth factor-beta type II receptor
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v207_n1_p40_Fazzini

Referencias:

  • Abraham, G.E., Swerdloff, R., Tulchinsky, D., Odell, W.D., Radioimmunoassay of plasma progesterone (1971) J Clin Endocrinol Metab, 32, pp. 619-624
  • Arai, K.Y., Ohshima, K., Arai, K., Uehara, K., Taya, K., Dynamics of messenger RNAs encoding inhibin-activin subunits and follistatin in the ovary during the rat estrous cycle (2002) Biol Reprod, 66, pp. 1119-1126
  • Bernath, V.A., Muro, A.F., Vitullo, A.D., Bley, M.A., Barafiao, J.L., Kornblihtt, A.R., Cyclic AMP inhibit fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element dependent transcriptional activation (1990) J Biol Chem, 265, pp. 18219-18226
  • Chiappe, M.E., Lattanzi, A.A., Colman-Lerner, A.A., Barafiao, J.L., Saragueta, P.E., Expression of 3-hydroxysteroid dehydrogenase in early bovine embryo development (2002) Biol Reprod, 61, pp. 135-141
  • Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (1987) Anal Biochem, 162, pp. 156-159
  • Colman-Lerner, A.A., Salamone, D.F., Chiappe, M.E., Barafiao, J.L., Comparative studies between freshly isolated and spontaneously immortalized bovine granulosa cells: Protein secretion, steroid metabolism, and responsiveness to growth factors (1995) J Cell Physiol, 164, pp. 395-403
  • Colman-Lerner, A.A., Fischman, M.L., Lanuza, G.M., Montgomery Bissell, D., Kornblihtt, A.R., Barafiao, J.L., Evidence for a role of the alternatively spliced ED-I sequence of fibronectin during ovarian follicular development (1999) Endocrinology, 140, pp. 2541-2548
  • Dalkin, A.C., Haisenleder, D.J., Yasin, M., Gilrain, J.T., Marshall, J.C., Pituitary activin receptor subtypes and follistatin gene expression in female rats: Differential regulation by activin and follistatin (1996) Endocrinology, 137, pp. 548-554
  • Danielpour, D., Dart, L.L., Flanders, K.C., Roberts, A.B., Sporn, M.B., Immunodetection and quantitation of the two forms of transforming growth factor-beta (TGF-beta 1 and TGF-beta 2) secreted by cells in culture (1989) J Cell Physiol, 138, pp. 79-86
  • Davis, A.J., Johnson, P.A., Expression pattern of messenger ribonucleic acid for follistatin and the inhibin/activin subunits during follicular and testicular development in Gallus domesticus (1998) Biol Reprod, 59, pp. 271-277
  • Delean, A., Munson, P.J., Rodbard, D., Simultaneous analysis of families of sigmoidal curves: Application to bioassay, radioligand assay, and physiological dose-response curves (1978) Am J Physiol, 235, pp. E97-E102
  • Erämaa, M., Ritvos, O., Transforming growth factor-β1 and -β2 induce inhibin and activin βB-subunit messenger ribonucleic acid levels in cultured human granulosa-luteal cells (1996) Fertil Steril, 65, pp. 954-960
  • Findlay, J.K., Drummond, A.E., Dyson, M.L., Baillie, A.J., Robertson, D.M., Ethier, J.F., Recruitment and development of the follicle; the roles of the transforming growth factor-β superfamily (2002) Mol Cell Endocrinol, 191, pp. 35-43
  • Glister, C., Tannetta, D.S., Groome, N.P., Knight, P.G., Interactions between follicle-stimulating hormone and growth factors in modulating secretion of steroids and ihibin-related peptides by nonluteinized bovine granulosa cells (2001) Biol Reprod, 65, pp. 1020-1028
  • Guo, Q., Kumar, T.R., Woodruff, T., Hadsell, L.A., Demayo, F.J., Matzuk, M.M., Overexpression of mouse follistatin causes reproductive defects in transgenic mice (1998) Mol Endocrinol, 12, pp. 96-106
  • Houde, A., Lussier, J.G., Ethier, J., Gagnon, C., Silversides, D.W., Cloning and tissue expression of bovine follistatin cDNA (1994) Mol Reprod Dev, 37, pp. 391-397
  • Hutchinson, L.A., Findlay, J.K., De Vos, F.L., Robertson, D.M., Effects of bovine inhibin, transforming growth factor-beta and bovine activin-A on granulosa cell differentiation (1987) Biochem Biophys Res Commun, 146, pp. 1405-1412
  • Iemura, S., Yamamoto, T.S., Takagi, C., Uchiyama, H., Natsume, T., Shimasaki, S., Sugino, H., Ueno, N., Direct binding of follistatin to a complex of bone-morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo (1998) Proc Natl Acad Sci USA, 95, pp. 9337-9342
  • Izadyar, F., Dijkstra, G., Van Tol, H.T., Van Den Eijnden-Van Raaij, A.J., Van Den Hurk, R., Colenbrander, B., Bebers, M.M., Immunohistochemical localization and mRNA expression of activin, inhibin, follistatin, and activin receptor in bovine cumulus-oocyte complexes during in vitro maturation (1998) Mol Reprod Dev, 49, pp. 186-195
  • Jorgez, C.J., Klysik, M., Jamin, S.P., Behringer, R.R., Matzuk, M.M., Granulosa cell-specific inactivation of follistatin causes female fertility defects (2004) Mol Endocrinol, 18, pp. 953-967
  • Klein, R., Robertson, D.M., Shukovski, L., Findlay, J.K., De Kretser, D.M., The radioimmunoassay of follicle-stimulating hormone (FSH)-suppressing protein (FSP): Stimulation of bovine granulosa cell FSP secretion by FSH (1991) Endocrinology, 28, pp. 1048-1056
  • Knight, P.G., Glister, C., Local roles of TGF-beta superfamily members in the control of ovarian follicle development (2003) Anim Reprod Sci, 78, pp. 165-183
  • Knight, P.G., Muttukrishna, S., Groome, N.P., Development and application of a two site enzyme immunoassay for the determination of "total" activin-A concentrations in serum and follicular fluid (1996) J Endocrinology, 148, pp. 267-279
  • Lapolt, P.S., Piquette, G.N., Soto, D., Sincich, C., Hsueh, A.J.W., Hsueh, A.J., Regulation of inhibin subunit messenger ribonucleic acid levels by gonadotropins, growth factors, and gonadotropin-releasing hormone in cultured rat granulosa cells (1990) Endocrinology, 127, pp. 823-831
  • Li, R., Phillips, D.M., Mather, J.P., Activin promotes ovarian follicle development in vitro (1995) Endocrinology, 136, pp. 849-856
  • Li, M.D., Depaolo, L.V., Ford, J.J., Expression of follistatin and inhibin/activin subunit genes in porcine follicles (1997) Biol Reprod, 57, pp. 112-118
  • Lindsell, C.E., Misra, V., Murphy, B.D., Regulation of follistatin messenger ribonucleic acid in porcine granulosa cells by epidermal growth factor and the protein kinase-C pathway (1993) Endocrinology, 132, pp. 1630-1636
  • Lindsell, C.E., Misra, V., Murphy, B.D., Regulation of follistatin gene expression in the ovary and in primary cultures of porcine granulosa cells (1994) J Reprod Fertil, 100, pp. 591-597
  • Lobb, D.K., Dorrington, J.H., Intraovarian regulation of follicular development (1992) Anim Reprod Sci, 28, pp. 343-354
  • Majdic, G., McNeilly, A.S., Sharpe, R.M., Evans, L.R., Groome, N.P., Saunders, P.T., Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat (1997) Endocrinology, 138, pp. 2136-2147
  • Massagué, J., The transforming growth factor-β family (1990) Annu Rev Cell Biol, 6, pp. 597-641
  • Massagué, J., How cells read TGF-beta signals (2000) Nat Rev Mol Cell Biol, 1, pp. 169-178
  • Matzuk, M.M., Lu, N., Vogel, H., Sellheyer, K., Roop, D.R., Bradley, A., Multiple defects and perinatal death in mice deficient in follistatin (1995) Nature, 74, pp. 360-363
  • Michel, U., Albiston, A., Findlay, J.K., Rat follistatin: Gonadal and extragonadal expression and evidence for alternative splicing (1990) Biochem Biophys Res Commun, 173, pp. 401-407
  • Michel, U., McMaster, J.W., Findlay, J.K., Regulation of steady-state follistatin mRNA levels in rat granulosa cells in vitro (1992) J Mol Endocrinol, 9, pp. 147-156
  • Minayaga, K., Shimasaki, S., Structural and functional characterization of the rat follistatin (activin-binding protein) gene promoter (1993) Mol Cell Endocrinol, 92, pp. 99-109
  • Miro, F., Hillier, S.G., Modulation of granulosa cell deoxyribonucleic acid synthesis and differentiation by activin (1996) Endocrinology, 137, pp. 464-468
  • Miro, F., Smyth, C.D., Hillier, S.G., Development-related effects of recombinant activin on steroid synthesis in rat granulosa cells (1991) Endocrinology, 129, pp. 3388-3394
  • Mulheron, G.W., Schomberg, D.W., The intraovarian transforming growth factor system (1993) The Ovary, pp. 337-361. , Adashi EY, Leung PCK, editors, NY: Raven Press Ltd
  • Nilsson, E.E., Doraiswamy, V., Skinner, M.K., Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development (2003) Mol Reprod Dev, 66, pp. 237-246
  • Otsuka, F., Moore, R.K., Iemura, S., Ueno, N., Shimasaki, S., Follistatin inhibits the function of the oocyte-derived factor BMP-16 (2001) Biochem Biophys Res Commun, 289, pp. 961-966
  • Rabinovici, J., Spencer, S.J., Jaffe, R.B., Recombinant human activin-A promotes proliferation of human luteinized preovulatory granulosa cells in vitro (1990) J Clin Endocrinol Metab, 71, pp. 1396-1398
  • Saito, S., Nakamura, T., Titani, K., Sugino, H., Production of activin-binding protein by rat granulosa cells in vitro (1991) Biochem Biophys Res Commun, 176, pp. 413-422
  • Saragüeta, P.E., Lanuza, G.M., Barañao, J.L., Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation (2002) Biol Reprod, 66, pp. 1862-1868
  • Shibanuma, M., Mashimo, J., Mita, A., Kuroki, T., Nose, K., Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-β1 regulated genes, one of which seems to encode a follistatin-related polypeptide (1993) Eur J Biochem, 217, pp. 13-19
  • Shimasaki, S., Koga, M., Buscaglia, M.L., Simmons, D.M., Bicksak, T.A., Ling, N., Follistatin gene expression in the ovary and extragonadal tissues (1989) Mol Endocrinol, 3, pp. 651-660
  • Shimasaki, S., Zachow, R.J., Li, D., Kim, H., Iemura, S., Ueno, N., Sampath, K., Erickson, G.F., A functional bone morphogenetic protein system in the ovary (1999) Proc Natl Acad Sci USA, 96, pp. 7282-7287
  • Shintani, Y., Dyson, M., Drummond, A.E., Findlay, J.K., Regulation of follistatin production by rat granulosa cells in vitro (1997) Endocrinology, 138, pp. 2544-2551
  • Shukovski, L., Findlay, J.K., Activin-A inhibits oxitocyn and progesterone production by preovulatory bovine granulosa cells in vitro (1990) Endocrinology, 126, pp. 2222-2224
  • Shukovski, L., Findlay, J.K., Robertson, D.M., The effect of follicle-stimulating hormone-suppressing protein or follistatin on luteinizing bovine granulosa cells in vitro and its antagonistic effect on the action of activin (1991) Endocrinology, 129, pp. 3395-3402
  • Shukovski, L., Zhang, Z.W., Michel, U., Findlay, J.K., Expression of mRNA for follicle-stimulating hormone suppressing protein in ovarian tissues of cows (1992) J Reprod Fertil, 95, pp. 861-867
  • Shukovski, L., Keren-Tal, I., Dantes, A., Amsterdam, A., Regulation of follistatin messenger ribonucleic acid in steroidogenic rat granulosa cell lines (1995) Endocrinology, 136, pp. 2889-2895
  • Skinner, M.K., Transforming growth factor production and action in the ovarian follicle: Theca cell-granulosa cell interactions (1989) Growth Factors and the Ovary, pp. 141-150. , Flirshfield AN, editor. New York: Plenum Press
  • Tano, M., Minegishi, T., Nakamura, K., Nakamura, M., Karino, S., Miyamoto, K., Ibuki, Y., Regulation of follistatin messenger ribonucleic acid in cultured rat granulosa cells (1995) Mol Cell Endocrinol, 109, pp. 167-174
  • Tuuri, T., Ritvos, O., Regulation of the activin-binding protein follistatin in cultured human luteinizing granulosa cells: Characterization of the effects of follicle stimulating hormone, prostaglandin E2, and different growth factors (1995) Biol Reprod, 53, pp. 1508-1516
  • Tuuri, T., Eramaa, M., Hilden, K., Ritvos, O., Activin-binding protein follistatin messenger ribonucleic acid and secreted protein levels are induced by chorionic gonadotropin in cultured human granulosa-luteal cells (1994) Endocrinology, 135, pp. 2196-2203
  • Welt, C., Sidis, Y., Keutmann, H., Schneyer, A., Activins, inhibins, and follistatins: From endocrinology to signaling. A paradigm for the new millennium (2002) Exp Biol Med (Maywood), 227, pp. 724-752
  • Zhang, Y.Q., Kanzaki, M., Shibata, H., Kojima, I., Regulation of the expression of follistatin in rat hepatocytes (1997) Biochim Biophys Acta, 1354, pp. 204-210

Citas:

---------- APA ----------
Fazzini, M., Vallejo, G., Colman-Lerner, A., Trigo, R., Campo, S., Barañao, J.L.S. & Saragüeta, P.E. (2006) . Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation. Journal of Cellular Physiology, 207(1), 40-48.
http://dx.doi.org/10.1002/jcp.20533
---------- CHICAGO ----------
Fazzini, M., Vallejo, G., Colman-Lerner, A., Trigo, R., Campo, S., Barañao, J.L.S., et al. "Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation" . Journal of Cellular Physiology 207, no. 1 (2006) : 40-48.
http://dx.doi.org/10.1002/jcp.20533
---------- MLA ----------
Fazzini, M., Vallejo, G., Colman-Lerner, A., Trigo, R., Campo, S., Barañao, J.L.S., et al. "Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation" . Journal of Cellular Physiology, vol. 207, no. 1, 2006, pp. 40-48.
http://dx.doi.org/10.1002/jcp.20533
---------- VANCOUVER ----------
Fazzini, M., Vallejo, G., Colman-Lerner, A., Trigo, R., Campo, S., Barañao, J.L.S., et al. Transforming growth factor β1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation. J. Cell. Physiol. 2006;207(1):40-48.
http://dx.doi.org/10.1002/jcp.20533