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Abstract:

Lovastatin (LOV), a hydroxy‐methylglutaryl‐coenzyme A (HMGCoA) reductase competitive inhibitor, blocks epidermal growth factor (EGF)— or prostaglandin F2α (PGF2α)—induced mitogenesis in confluent resting Swiss 3T3 cells. This inhibition occurs even in the presence of insulin, which potentiates the action of these mitogens in such cells. LOV exerts its effect in a 2–80 μM concentration range, with both mitogens attaining 50% inhibition at 7.5 μM. LOV exerted its effect within 0–8 h following mitogenic induction. Mevanolactone (10–80 μM) in the presence of LOV could reverse LOV inhibition within a similar time period. LOV‐induced blockage of PGF2α response is reflected in a decrease in the rate of cell entry into S phase. Neither cholesterol, ubiquinone, nor dolichols of various lengths could revert LOV blockage. In EGF‐ or PGF2α‐stimulated cells, LOV did not inhibit [3H]leucine or [3H]mannose incorporation into proteins, while tunicamycin, an inhibitor of N′ glycosylation, prevented this last phenomenon. Thus, it appears that LOV exerts its action neither by inhibiting unspecific protein synthesis nor by impairing the N′ glycosylation process. These findings strongly suggest that either EGF or PGF2α stimulations generate early cell cycle signals which induce mevalonate formation, N′ glycoprotein synthesis, and proliferation. The causal relationship of these events to various mechanisms controlling the onset of DNA synthesis is also discussed. © 1995 Wiley‐Liss, Inc. Copyright © 1995 Wiley‐Liss, Inc.

Registro:

Documento: Artículo
Título:Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells
Autor:Ortiz, M.B.; Goin, M.; de Alzaga, M.B.G.; Hammarstrom, S.; de Asua, L.J.
Filiación:Instituto de Investigaciones En Ingeniería Genética Y Biología Molecular, Buenos Aires, 1428, Argentina
Department of Cell Biology, Faculty of Health Science, University of Linkoping, Linkoping, Sweden
Palabras clave:cholesterol; dolichol; epidermal growth factor; insulin; mevalonic acid; mevinolin; prostaglandin f2 alpha; tunicamycin; ubiquinone; animal cell; article; cell cycle; cell strain 3t3; controlled study; mitogenesis; mouse; nonhuman; priority journal; 3T3 Cells; Animal; Dinoprost; Dose-Response Relationship, Drug; Epidermal Growth Factor; Glycosylation; Lovastatin; Mevalonic Acid; Mice; Mitosis; S Phase; Support, Non-U.S. Gov't; Animalia
Año:1995
Volumen:162
Número:1
Página de inicio:139
Página de fin:146
DOI: http://dx.doi.org/10.1002/jcp.1041620117
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
ISSN:00219541
CAS:Dinoprost, 551-11-1; Epidermal Growth Factor, 62229-50-9; Lovastatin, 75330-75-5; Mevalonic Acid, 150-97-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v162_n1_p139_Ortiz

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Citas:

---------- APA ----------
Ortiz, M.B., Goin, M., de Alzaga, M.B.G., Hammarstrom, S. & de Asua, L.J. (1995) . Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells. Journal of Cellular Physiology, 162(1), 139-146.
http://dx.doi.org/10.1002/jcp.1041620117
---------- CHICAGO ----------
Ortiz, M.B., Goin, M., de Alzaga, M.B.G., Hammarstrom, S., de Asua, L.J. "Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells" . Journal of Cellular Physiology 162, no. 1 (1995) : 139-146.
http://dx.doi.org/10.1002/jcp.1041620117
---------- MLA ----------
Ortiz, M.B., Goin, M., de Alzaga, M.B.G., Hammarstrom, S., de Asua, L.J. "Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells" . Journal of Cellular Physiology, vol. 162, no. 1, 1995, pp. 139-146.
http://dx.doi.org/10.1002/jcp.1041620117
---------- VANCOUVER ----------
Ortiz, M.B., Goin, M., de Alzaga, M.B.G., Hammarstrom, S., de Asua, L.J. Mevalonate dependency of the early cell cycle mitogenic response to epidermal growth factor and prostaglandin F2α in Swiss mouse 3T3 cells. J. Cell. Physiol. 1995;162(1):139-146.
http://dx.doi.org/10.1002/jcp.1041620117