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Abstract:

In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc.

Registro:

Documento: Artículo
Título:Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
Autor:Medrano, E.E.; Goldemberg, S.H.; Algranati, I.D.
Filiación:Instituto de Investigaciones Bioquimicas "Fundacion Campomar" and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:benzo[a]pyrene; eflornithine; polyamine; putrescine; radioisotope; spermidine; animal cell; cancer chemotherapy; cell culture; cell proliferation; cell transformation; drug therapy; in vitro study; mouse; nonhuman; reticuloendothelial system; therapy; thymidine h 3; Animal; Benzo(a)pyrene; Benzopyrenes; Carcinogens; Cell Division; Cell Transformation, Neoplastic; Cells, Cultured; DNA Replication; Eflornithine; Interphase; Kinetics; Mice; Mice, Inbred BALB C; Ornithine; Ornithine Decarboxylase; Putrescine; Support, Non-U.S. Gov't; Transcription, Genetic; Translation, Genetic
Año:1983
Volumen:117
Número:2
Página de inicio:141
Página de fin:147
DOI: http://dx.doi.org/10.1002/jcp.1041170202
Título revista:Journal of Cellular Physiology
Título revista abreviado:J. Cell. Physiol.
ISSN:00219541
CAS:benzo[a]pyrene, 50-32-8; eflornithine, 67037-37-0, 70052-12-9; putrescine, 110-60-1, 333-93-7; spermidine, 124-20-9, 334-50-9; Benzo(a)pyrene, 50-32-8; Benzopyrenes; Carcinogens; Eflornithine, 70052-12-9; Ornithine Decarboxylase, EC 4.1.1.17; Ornithine, 7006-33-9; Putrescine, 110-60-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219541_v117_n2_p141_Medrano

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Citas:

---------- APA ----------
Medrano, E.E., Goldemberg, S.H. & Algranati, I.D. (1983) . Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells. Journal of Cellular Physiology, 117(2), 141-147.
http://dx.doi.org/10.1002/jcp.1041170202
---------- CHICAGO ----------
Medrano, E.E., Goldemberg, S.H., Algranati, I.D. "Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells" . Journal of Cellular Physiology 117, no. 2 (1983) : 141-147.
http://dx.doi.org/10.1002/jcp.1041170202
---------- MLA ----------
Medrano, E.E., Goldemberg, S.H., Algranati, I.D. "Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells" . Journal of Cellular Physiology, vol. 117, no. 2, 1983, pp. 141-147.
http://dx.doi.org/10.1002/jcp.1041170202
---------- VANCOUVER ----------
Medrano, E.E., Goldemberg, S.H., Algranati, I.D. Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells. J. Cell. Physiol. 1983;117(2):141-147.
http://dx.doi.org/10.1002/jcp.1041170202