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Repression of mRNA translation is linked to the formation of specific cytosolic foci such as stress granules and processing bodies, which store or degrade mRNAs. In neurons, synaptic activity regulates translation at the post-synapse and this is important for plasticity. N-methyl-D-aspartate (NMDA) receptor stimulation downregulates translation, and we speculate that this is linked to the formation of unknown mRNA-silencing foci. Here, we show that the 5'-3' exoribonuclease XRN1 forms discrete clusters associated with the post-synapse that are different from processing bodies or stress granules, and we named them synaptic XRN1 bodies (SX-bodies). Using primary neurons, we found that the SX-bodies respond to synapse stimulation and that their formation correlates inversely with the local translation rate. SX-bodies increase in size and number upon NMDA stimulation, and metabotropic glutamate receptor activation provokes SX-body dissolution, along with increased translation. The response is specific and the previously described Smaug1 foci and FMRP granules show a different response. Finally, XRN1 knockdown impairs the translational repression triggered by NMDA. Collectively, these observations support a role for the SX-bodies in the reversible masking and silencing of mRNAs at the synapse. © 2015. Published by The Company of Biologists Ltd.


Documento: Artículo
Título:Synaptic control of mRNA translation by reversible assembly of XRN1 bodies
Autor:Luchelli, L.; Thomas, M.G.; Boccaccio, G.L.
Filiación:Fundación Instituto Leloir, Buenos Aires, C1405BWE, Argentina
Instituto de Investigaciones Bioquimicas Buenos Aires-CONICET, Buenos Aires, C1405BWE, Argentina
Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Buenos Aires, C1428EHA, Argentina
Palabras clave:P body; RNA granule; Stress granule; Synapse; XRN1; exoribonuclease; messenger RNA; metabotropic receptor; n methyl dextro aspartic acid; synapsin; unclassified drug; XRN1 protein; DNA binding protein; exoribonuclease; messenger RNA; metabotropic receptor; n methyl dextro aspartic acid receptor; Xrn1 protein, mouse; animal experiment; animal tissue; Article; cell body; cellular distribution; controlled study; dendrite; dendritic spine; hippocampal CA1 region; hippocampal CA3 region; hippocampal neuronal culture; immunofluorescence; nonhuman; priority journal; protein localization; protein processing; protein synthesis; rat; RNA translation; synapse; Western blotting; amino acid sequence; animal; cell culture; cell organelle; gene expression regulation; genetics; metabolism; molecular genetics; mouse; nerve cell; Sprague Dawley rat; synapse; Amino Acid Sequence; Animals; Cells, Cultured; DNA-Binding Proteins; Exoribonucleases; Gene Expression Regulation; Mice; Molecular Sequence Data; Neurons; Organelles; Protein Biosynthesis; Rats, Sprague-Dawley; Receptors, Metabotropic Glutamate; Receptors, N-Methyl-D-Aspartate; RNA, Messenger; Synapses
Página de inicio:1542
Página de fin:1554
Título revista:Journal of Cell Science
Título revista abreviado:J. Cell Sci.
CAS:exoribonuclease, 37288-24-7; n methyl dextro aspartic acid, 6384-92-5; DNA-Binding Proteins; Exoribonucleases; Receptors, Metabotropic Glutamate; Receptors, N-Methyl-D-Aspartate; RNA, Messenger; Xrn1 protein, mouse


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---------- APA ----------
Luchelli, L., Thomas, M.G. & Boccaccio, G.L. (2015) . Synaptic control of mRNA translation by reversible assembly of XRN1 bodies. Journal of Cell Science, 128(8), 1542-1554.
---------- CHICAGO ----------
Luchelli, L., Thomas, M.G., Boccaccio, G.L. "Synaptic control of mRNA translation by reversible assembly of XRN1 bodies" . Journal of Cell Science 128, no. 8 (2015) : 1542-1554.
---------- MLA ----------
Luchelli, L., Thomas, M.G., Boccaccio, G.L. "Synaptic control of mRNA translation by reversible assembly of XRN1 bodies" . Journal of Cell Science, vol. 128, no. 8, 2015, pp. 1542-1554.
---------- VANCOUVER ----------
Luchelli, L., Thomas, M.G., Boccaccio, G.L. Synaptic control of mRNA translation by reversible assembly of XRN1 bodies. J. Cell Sci. 2015;128(8):1542-1554.