Artículo

Otero, M.G.; Alloatti, M.; Cromberg, L.E.; Almenar-Queralt, A.; Encalada, S.E.; Devoto, V.M.P.; Bruno, L.; Goldstein, L.S.B.; Falzone, T.L. "Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function" (2014) Journal of Cell Science. 127(7):1537-1549
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Abstract:

Protein degradation by the ubiquitin-proteasome system in neurons depends on the correct delivery of the proteasome complex. In neurodegenerative diseases, aggregation and accumulation of proteins in axons link transport defects with degradation impairments; however, the transport properties of proteasomes remain unknown. Here, using in vivo experiments, we reveal the fast anterograde transport of assembled and functional 26S proteasome complexes. A high-resolution tracking system to follow fluorescent proteasomes revealed three types of motion: actively driven proteasome axonal transport, diffusive behavior in a viscoelastic axonema and proteasome-confined motion. We show that active proteasome transport depends on motor function because knockdown of the KIF5B motor subunit resulted in impairment of the anterograde proteasome flux and the density of segmental velocities. Finally, we reveal that neuronal proteasomes interact with intracellular membranes and identify the coordinated transport of fluorescent proteasomes with synaptic precursor vesicles, Golgi-derived vesicles, lysosomes and mitochondria. Taken together, our results reveal fast axonal transport as a new mechanism of proteasome delivery that depends on membrane cargo 'hitch-hiking' and the function of molecular motors. We further hypothesize that defects in proteasome transport could promote abnormal protein clearance in neurodegenerative diseases. © 2014.Published by The Company of Biologists Ltd.

Registro:

Documento: Artículo
Título:Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function
Autor:Otero, M.G.; Alloatti, M.; Cromberg, L.E.; Almenar-Queralt, A.; Encalada, S.E.; Devoto, V.M.P.; Bruno, L.; Goldstein, L.S.B.; Falzone, T.L.
Filiación:Instituto de Biología Celular y Neurociencias (UBA-CONICET), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires CP 1121, Argentina
Department of Cellular and Molecular Medicine, School of Medicine, University of California San Diego, La Jolla, CA 92093, United States
Departamento de Física y IFIBA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires CP 1428, Argentina
Department of Molecular and Experimental Medicine, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, United States
Palabras clave:Axonal transport; Kinesin-1; Membrane interaction; Molecular motors; Proteasome; Vesicles; molecular motor; proteasome; ubiquitin; proteasome; animal tissue; article; axoneme; clearance; controlled study; degenerative disease; diffusion; Golgi complex; in vivo study; intracellular membrane; lysosome; mitochondrion; molecular interaction; motor unit; mouse; nerve fiber transport; nonhuman; priority journal; protein degradation; synapse vesicle; viscoelasticity; animal; C57BL mouse; cell culture; cytology; hippocampus; intracellular membrane; metabolism; nerve fiber; nerve fiber transport; physiology; sciatic nerve; synaptosome; transport at the cellular level; Animals; Axonal Transport; Axons; Biological Transport; Cells, Cultured; Hippocampus; Intracellular Membranes; Mice; Mice, Inbred C57BL; Proteasome Endopeptidase Complex; Sciatic Nerve; Synaptic Vesicles; Synaptosomes
Año:2014
Volumen:127
Número:7
Página de inicio:1537
Página de fin:1549
DOI: http://dx.doi.org/10.1242/jcs.140780
Título revista:Journal of Cell Science
Título revista abreviado:J. Cell Sci.
ISSN:00219533
CODEN:JNCSA
CAS:proteasome, 140879-24-9; ubiquitin, 60267-61-0; Proteasome Endopeptidase Complex
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v127_n7_p1537_Otero

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Citas:

---------- APA ----------
Otero, M.G., Alloatti, M., Cromberg, L.E., Almenar-Queralt, A., Encalada, S.E., Devoto, V.M.P., Bruno, L.,..., Falzone, T.L. (2014) . Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function. Journal of Cell Science, 127(7), 1537-1549.
http://dx.doi.org/10.1242/jcs.140780
---------- CHICAGO ----------
Otero, M.G., Alloatti, M., Cromberg, L.E., Almenar-Queralt, A., Encalada, S.E., Devoto, V.M.P., et al. "Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function" . Journal of Cell Science 127, no. 7 (2014) : 1537-1549.
http://dx.doi.org/10.1242/jcs.140780
---------- MLA ----------
Otero, M.G., Alloatti, M., Cromberg, L.E., Almenar-Queralt, A., Encalada, S.E., Devoto, V.M.P., et al. "Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function" . Journal of Cell Science, vol. 127, no. 7, 2014, pp. 1537-1549.
http://dx.doi.org/10.1242/jcs.140780
---------- VANCOUVER ----------
Otero, M.G., Alloatti, M., Cromberg, L.E., Almenar-Queralt, A., Encalada, S.E., Devoto, V.M.P., et al. Fast axonal transport of the proteasome complex depends on membrane interaction and molecular motor function. J. Cell Sci. 2014;127(7):1537-1549.
http://dx.doi.org/10.1242/jcs.140780