Artículo

Papusheva, E.; de Queiroz, F.M.; Dalous, J.; Han, Y.; Esposito, A.; Jares-Erijman, E.A.; Jovin, T.M.; Bunt, G. "Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility" (2009) Journal of Cell Science. 122(5):656-666
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Abstract:

Focal adhesion kinase (FAK) controls cellular adhesion and motility processes by its tight link to integrin- and extracellular-matrix-mediatedTo explore the dynamics of the regulation of FAK, we constructed a FRET-based probe that visualizes conformational rearrangements of the FERM domain of FAK in living cells. The sensor reports on an integrin-mediated conformational change in FAK following cellular adhesion. The perturbation is kinase-independent and involves the polybasic KAKTLR sequence in the FERM domain. It is manifested by an increased FRET signal and is expressed primarily in focal adhesions, and to a lesser extent in the cytoplasm. The conformational change in the FERM domain of FAK is observed in two consecutive phases during spreading - early and late - and is enriched in fully adhered motile cells at growing and sliding peripheral focal-adhesion sites, but not in stable or retracting focal adhesions. Inhibition of the actomyosin system indicates the involvement of tension signaling induced by Rho-associated kinase, rather than by myosin light-chain kinase, in the modulation of the FERM response. We conclude that the heterogeneous conformation of the FERM domain in focal adhesions of migrating cells reflects a complex regulatory mechanism for FAK that appears to be under the influence of cellular traction forces.

Registro:

Documento: Artículo
Título:Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility
Autor:Papusheva, E.; de Queiroz, F.M.; Dalous, J.; Han, Y.; Esposito, A.; Jares-Erijman, E.A.; Jovin, T.M.; Bunt, G.
Filiación:Molecular Biology of Neuronal Signals, Max-Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
Department of Neuro- and Sensory Physiology, University Medicine Göttingen, 37073 Göttingen, Germany
Department of Cellular Biophysics, European Neuroscience Institute, 37073 Göttingen, Germany
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
DFG Research Center for Molecular Physiology of the Brain (CMPB), 37073 Göttingen, Germany
Laboratory of Cellular Dynamics, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany
Palabras clave:FAK; FERM conformation; Force; FRET; Migration; ROCK; focal adhesion kinase; integrin; myosin adenosine triphosphatase; myosin light chain kinase; Rho kinase; fluorescent dye; focal adhesion kinase; amino acid sequence; animal cell; article; cell adhesion; cell migration; cell motility; cell spreading; conformational transition; controlled study; embryo; enzyme regulation; fibroblast culture; fluorescence resonance energy transfer; focal adhesion; molecular probe; mouse; nonhuman; priority journal; protein analysis; protein conformation; protein domain; protein function; signal transduction; animal; cell adhesion; cell culture; cell motion; chemistry; cytology; fibroblast; focal adhesion; genetics; human; metabolism; mouse mutant; physiology; protein conformation; Animals; Cell Adhesion; Cell Movement; Cells, Cultured; Fibroblasts; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Focal Adhesion Protein-Tyrosine Kinases; Focal Adhesions; Humans; Mice; Mice, Knockout; Protein Conformation; Signal Transduction
Año:2009
Volumen:122
Número:5
Página de inicio:656
Página de fin:666
DOI: http://dx.doi.org/10.1242/jcs.028738
Título revista:Journal of Cell Science
Título revista abreviado:J. Cell Sci.
ISSN:00219533
CODEN:JNCSA
CAS:myosin light chain kinase, 51845-53-5; Fluorescent Dyes; Focal Adhesion Protein-Tyrosine Kinases, 2.7.1.112
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n5_p656_Papusheva

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Citas:

---------- APA ----------
Papusheva, E., de Queiroz, F.M., Dalous, J., Han, Y., Esposito, A., Jares-Erijman, E.A., Jovin, T.M.,..., Bunt, G. (2009) . Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility. Journal of Cell Science, 122(5), 656-666.
http://dx.doi.org/10.1242/jcs.028738
---------- CHICAGO ----------
Papusheva, E., de Queiroz, F.M., Dalous, J., Han, Y., Esposito, A., Jares-Erijman, E.A., et al. "Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility" . Journal of Cell Science 122, no. 5 (2009) : 656-666.
http://dx.doi.org/10.1242/jcs.028738
---------- MLA ----------
Papusheva, E., de Queiroz, F.M., Dalous, J., Han, Y., Esposito, A., Jares-Erijman, E.A., et al. "Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility" . Journal of Cell Science, vol. 122, no. 5, 2009, pp. 656-666.
http://dx.doi.org/10.1242/jcs.028738
---------- VANCOUVER ----------
Papusheva, E., de Queiroz, F.M., Dalous, J., Han, Y., Esposito, A., Jares-Erijman, E.A., et al. Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility. J. Cell Sci. 2009;122(5):656-666.
http://dx.doi.org/10.1242/jcs.028738