Artículo

Sosnik, J.; Miranda, P.V.; Spiridonov, N.A.; Yoon, S.-Y.; Fissore, R.A.; Johnson, G.R.; Visconti, P.E. "Tssk6 is required for Izumo relocalization and gamete fusion in the mouse" (2009) Journal of Cell Science. 122(15):2741-2749
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Abstract:

One of the most important processes in fertilization is the fusion of egg and sperm; however, the molecular mechanisms involved in this process are not well understood. So far, using genetic approaches, only two proteins have been demonstrated to be necessary for this process: Izumo in sperm and CD9 in the egg. Here we demonstrate that sperm produced by Tssk6 (Sstk)-null mice present defects that prevent the successful fertilization of eggs in vitro and the fusion to zona-pellucida-free eggs. Tssk6 is a member of the testis-specific serine kinase family of proteins and is expressed postmeiotically in male germ cells. In order for fusion to occur, during the process known as acrosome reaction Izumo needs to relocate from the anterior head to other regions, including the postacrosomal compartment. Tssk6-null sperm fails to relocate Izumo during the acrosome reaction. Agents that interfere with actin dynamics blocked the acrosome- reaction-associated translocation of Izumo that is required for fusion in wild-type sperm. Additionally, actin polymerization was compromised in Tssk6-null sperm. Taken together, our results indicate that Tssk6 is involved in sperm-egg fusion through the regulation of actin polymerization and changes in Izumo localization.

Registro:

Documento: Artículo
Título:Tssk6 is required for Izumo relocalization and gamete fusion in the mouse
Autor:Sosnik, J.; Miranda, P.V.; Spiridonov, N.A.; Yoon, S.-Y.; Fissore, R.A.; Johnson, G.R.; Visconti, P.E.
Filiación:Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, United States
Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, United States
Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires 1428, Argentina
Division of Therapeutic Proteins, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, United States
Palabras clave:Acrosome reaction; Fertilization; Gamete fusion; Izumo; Tssk6 (Sstk); actin; cell protein; izumo protein; protein serine kinase; testis specific serine kinase 6 protein; unclassified drug; acrosome; acrosome reaction; actin polymerization; animal cell; article; controlled study; female; fertilization in vitro; gamete; intracellular transport; male; mouse; nonhuman; priority journal; protein expression; protein function; protein localization; protein protein interaction; protein transport; spermatogenesis; wild type; zona pellucida; Animals; Blotting, Western; Calcium; Cell Fusion; Female; Fertilization in Vitro; Immunoblotting; Immunoenzyme Techniques; Immunoglobulins; Male; Membrane Proteins; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein-Serine-Threonine Kinases; Sperm-Ovum Interactions; Spermatozoa; Mus
Año:2009
Volumen:122
Número:15
Página de inicio:2741
Página de fin:2749
DOI: http://dx.doi.org/10.1242/jcs.047225
Título revista:Journal of Cell Science
Título revista abreviado:J. Cell Sci.
ISSN:00219533
CODEN:JNCSA
CAS:Calcium, 7440-70-2; Immunoglobulins; Izumo1 protein, mouse; Membrane Proteins; Protein-Serine-Threonine Kinases, 2.7.11.1; Tssk6 protein, mouse, 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n15_p2741_Sosnik

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Citas:

---------- APA ----------
Sosnik, J., Miranda, P.V., Spiridonov, N.A., Yoon, S.-Y., Fissore, R.A., Johnson, G.R. & Visconti, P.E. (2009) . Tssk6 is required for Izumo relocalization and gamete fusion in the mouse. Journal of Cell Science, 122(15), 2741-2749.
http://dx.doi.org/10.1242/jcs.047225
---------- CHICAGO ----------
Sosnik, J., Miranda, P.V., Spiridonov, N.A., Yoon, S.-Y., Fissore, R.A., Johnson, G.R., et al. "Tssk6 is required for Izumo relocalization and gamete fusion in the mouse" . Journal of Cell Science 122, no. 15 (2009) : 2741-2749.
http://dx.doi.org/10.1242/jcs.047225
---------- MLA ----------
Sosnik, J., Miranda, P.V., Spiridonov, N.A., Yoon, S.-Y., Fissore, R.A., Johnson, G.R., et al. "Tssk6 is required for Izumo relocalization and gamete fusion in the mouse" . Journal of Cell Science, vol. 122, no. 15, 2009, pp. 2741-2749.
http://dx.doi.org/10.1242/jcs.047225
---------- VANCOUVER ----------
Sosnik, J., Miranda, P.V., Spiridonov, N.A., Yoon, S.-Y., Fissore, R.A., Johnson, G.R., et al. Tssk6 is required for Izumo relocalization and gamete fusion in the mouse. J. Cell Sci. 2009;122(15):2741-2749.
http://dx.doi.org/10.1242/jcs.047225