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Inda, C.; Claro, P.A.S.; Bonfiglio, J.J.; Senin, S.A.; Maccarrone, G.; Turck, C.W.; Silberstein, S. "Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling" (2016) Journal of Cell Biology. 214(2):181-195
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Abstract:

Corticotropin-releasing hormone receptor 1 (CRHR1) activates G protein-dependent and internalization-dependent signaling mechanisms. Here, we report that the cyclic AMP (cAMP) response of CRHR1 in physiologically relevant scenarios engages separate cAMP sources, involving the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). cAMP produced by tmACs and sAC is required for the acute phase of extracellular signal regulated kinase 1/2 activation triggered by CRH-stimulated CRHR1, but only sAC activity is essential for the sustained internalization-dependent phase. Thus, different cAMP sources are involved in different signaling mechanisms. Examination of the cAMP response revealed that CRH-activated CRHR1 generates cAMP after endocytosis. Characterizing CRHR1 signaling uncovered a specific link between CRH-activated CRHR1, sAC, and endosome-based signaling. We provide evidence of sAC being involved in an endocytosis-dependent cAMP response, strengthening the emerging model of GPCR signaling in which the cAMP response does not occur exclusively at the plasma membrane and introducing the notion of sAC as an alternative source of cAMP. © 2016 Inda et al.

Registro:

Documento: Artículo
Título:Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling
Autor:Inda, C.; Claro, P.A.S.; Bonfiglio, J.J.; Senin, S.A.; Maccarrone, G.; Turck, C.W.; Silberstein, S.
Filiación:Instituto de Investigación en Biomedicina de Buenos Aires-CON ICET, Partner Institute of the Max Planck Society, Buenos Aires, C1425FQD, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428EHA, Argentina
Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, 80804, Germany
Max Planck Institute for Biology of Ageing, Cologne, 50931, Germany
Palabras clave:adenylate cyclase; corticotropin releasing factor receptor 1; cyclic AMP; G protein coupled receptor; mitogen activated protein kinase 3; adenylate cyclase; bicarbonate; calcium; corticotropin releasing factor; corticotropin releasing factor receptor; corticotropin releasing factor receptor 1; cyclic AMP; cyclic AMP dependent protein kinase; guanine nucleotide exchange factor; animal cell; Article; cell interaction; controlled study; endocytosis; enzyme activation; enzyme activity; nonhuman; priority journal; protein expression; signal transduction; 3T3-L1 cell line; ACTH secreting cell; animal; cell membrane; drug effects; enzymology; human; metabolism; mouse; rat; solubility; 3T3-L1 Cells; Adenylyl Cyclases; Animals; Bicarbonates; Calcium; Cell Membrane; Corticotrophs; Corticotropin-Releasing Hormone; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Endocytosis; Guanine Nucleotide Exchange Factors; Humans; Mice; Rats; Receptors, Corticotropin-Releasing Hormone; Signal Transduction; Solubility
Año:2016
Volumen:214
Número:2
Página de inicio:181
Página de fin:195
DOI: http://dx.doi.org/10.1083/jcb.201512075
Título revista:Journal of Cell Biology
Título revista abreviado:J. Cell Biol.
ISSN:00219525
CODEN:JCLBA
CAS:adenylate cyclase, 9012-42-4; cyclic AMP, 60-92-4; mitogen activated protein kinase 3, 137632-07-6; bicarbonate, 144-55-8, 71-52-3; calcium, 7440-70-2, 14092-94-5; corticotropin releasing factor, 9015-71-8, 178359-01-8, 79804-71-0, 86297-72-5, 86784-80-7; cyclic AMP dependent protein kinase; Adenylyl Cyclases; Bicarbonates; Calcium; Corticotropin-Releasing Hormone; CRF receptor type 1; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Guanine Nucleotide Exchange Factors; Receptors, Corticotropin-Releasing Hormone
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219525_v214_n2_p181_Inda

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Citas:

---------- APA ----------
Inda, C., Claro, P.A.S., Bonfiglio, J.J., Senin, S.A., Maccarrone, G., Turck, C.W. & Silberstein, S. (2016) . Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. Journal of Cell Biology, 214(2), 181-195.
http://dx.doi.org/10.1083/jcb.201512075
---------- CHICAGO ----------
Inda, C., Claro, P.A.S., Bonfiglio, J.J., Senin, S.A., Maccarrone, G., Turck, C.W., et al. "Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling" . Journal of Cell Biology 214, no. 2 (2016) : 181-195.
http://dx.doi.org/10.1083/jcb.201512075
---------- MLA ----------
Inda, C., Claro, P.A.S., Bonfiglio, J.J., Senin, S.A., Maccarrone, G., Turck, C.W., et al. "Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling" . Journal of Cell Biology, vol. 214, no. 2, 2016, pp. 181-195.
http://dx.doi.org/10.1083/jcb.201512075
---------- VANCOUVER ----------
Inda, C., Claro, P.A.S., Bonfiglio, J.J., Senin, S.A., Maccarrone, G., Turck, C.W., et al. Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. J. Cell Biol. 2016;214(2):181-195.
http://dx.doi.org/10.1083/jcb.201512075