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Abstract:

Confocal microscopy images revealed that the tetratricopeptide repeat motif (TPR) domain immunophilin FKBP51 shows colocalization with the specific mitochondrial marker Mito-Tracker. Signal specificity was tested with different antibodies and by FKBP51 knockdown. This unexpected subcellular localization of FKBP51 was confirmed by colocalization studies with other mitochondrial proteins, biochemical fractionation, and electron microscopy imaging. Interestingly, FKBP51 forms complexes in mitochondria with the glucocorticoid receptor and the Hsp90/Hsp70-based chaperone heterocomplex. Although Hsp90 inhibitors favor FKBP51 translocation from mitochondria to the nucleus in a reversible manner, TPR domain-deficient mutants of FKBP51 are constitutively nuclear and fully excluded from mitochondria, suggesting that a functional TPR domain is required for its mitochondrial localization. FKBP51 overexpression protects cells against oxidative stress, whereas FKBP51 knockdown makes them more sensitive to injury. In summary, this is the first demonstration that FKBP51 is a major mitochondrial factor that undergoes nuclear-mitochondrial shuttling, an observation that may be related to antiapoptotic mechanisms triggered during the stress response. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

Registro:

Documento: Artículo
Título:The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress
Autor:Gallo, L.I.; Lagadari, M.; Piwien-Pilipuk, G.; Galigniana, M.D.
Filiación:Inst. de Biologia Y Med. Exp./Consejo Nac. de Investigaciones Cientificas Y Tecnicas (IBYME/CONICET), C1428ADN Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Dept. of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, United States
Palabras clave:Antiapoptotic; Biochemical fractionation; Co-localization studies; Colocalization; Glucocorticoid receptor; Heat-shock protein; Immunophilins; Mitochondrial marker; Mitochondrial protein; Over-expression; Stress response; Subcellular localizations; Tetratricopeptide repeat motif; Confocal microscopy; Proteins; Mitochondria; chaperone; fk 506 binding protein 51; glucocorticoid receptor; heat shock protein 70; heat shock protein 90; immunophilin; mitochondrial protein; unclassified drug; article; cell nucleus; cell protection; cell strain 3T3; cellular distribution; cellular stress response; complex formation; controlled study; electron microscopy; gene silencing; mitochondrion; oxidative stress; priority journal; protein analysis; protein domain; protein expression; protein function; protein localization; protein transport; tetratricopeptide repeat; 3T3-L1 Cells; Active Transport, Cell Nucleus; Animals; Cell Nucleus; HSP70 Heat-Shock Proteins; HSP90 Heat-Shock Proteins; Humans; Mice; Mitochondria; Mitochondrial Proteins; Mutation; Oxidative Stress; Protein Structure, Tertiary; Receptors, Glucocorticoid; Tacrolimus Binding Proteins; Vasconcellea candicans
Año:2011
Volumen:286
Número:34
Página de inicio:30152
Página de fin:30160
DOI: http://dx.doi.org/10.1074/jbc.M111.256610
Título revista:Journal of Biological Chemistry
Título revista abreviado:J. Biol. Chem.
ISSN:00219258
CODEN:JBCHA
CAS:HSP70 Heat-Shock Proteins; HSP90 Heat-Shock Proteins; Mitochondrial Proteins; Receptors, Glucocorticoid; Tacrolimus Binding Proteins, 5.2.1.-; tacrolimus binding protein 5, 5.2.1.8
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219258_v286_n34_p30152_Gallo.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v286_n34_p30152_Gallo

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Citas:

---------- APA ----------
Gallo, L.I., Lagadari, M., Piwien-Pilipuk, G. & Galigniana, M.D. (2011) . The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress. Journal of Biological Chemistry, 286(34), 30152-30160.
http://dx.doi.org/10.1074/jbc.M111.256610
---------- CHICAGO ----------
Gallo, L.I., Lagadari, M., Piwien-Pilipuk, G., Galigniana, M.D. "The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress" . Journal of Biological Chemistry 286, no. 34 (2011) : 30152-30160.
http://dx.doi.org/10.1074/jbc.M111.256610
---------- MLA ----------
Gallo, L.I., Lagadari, M., Piwien-Pilipuk, G., Galigniana, M.D. "The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress" . Journal of Biological Chemistry, vol. 286, no. 34, 2011, pp. 30152-30160.
http://dx.doi.org/10.1074/jbc.M111.256610
---------- VANCOUVER ----------
Gallo, L.I., Lagadari, M., Piwien-Pilipuk, G., Galigniana, M.D. The 90-kDa heat-shock protein (Hsp90)-binding immunophilin FKBP51 is a mitochondrial protein that translocates to the nucleus to protect cells against oxidative stress. J. Biol. Chem. 2011;286(34):30152-30160.
http://dx.doi.org/10.1074/jbc.M111.256610