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Abstract:

Despite considerable progress in our understanding of the interplay between immune and endocrine systems, the role of thyroid hormones and their receptors in the control of adaptive immunity is still uncertain. Here, we investigated the role of thyroid hormone receptor (TR) β 1 signaling in modulating dendritic cell (DC) physiology and the intracellular mechanisms underlying these immunoregulatory effects. Exposure of DCs to triiodothyronine (T 3 ) resulted in a rapid and sustained increase in Akt phosphorylation independently of phosphatidylinositol 3-kinase activation, which was essential for supporting T 3 -induced DC maturation and interleukin (IL)-12 production. This effect was dependent on intact TRβ 1 signaling as small interfering RNA-mediated silencing of TRβ 1 expression prevented T 3 -induced DC maturation and IL-12 secretion as well as Akt activation and IκB-ε degradation. In turn, T 3 up-regulated TRβ 1 expression through mechanisms involving NF-κB, suggesting an autocrine regulatory loop to control hormone-dependent TRβ 1 signaling. These findings were confirmed by chromatin immunoprecipitation analysis, which disclosed a new functional NF-κB consensus site in the promoter region of the TRB1 gene. Thus, a T 3 -induced NF-κB-dependent mechanism controls TRβ 1 expression, which in turn signals DCs to promote maturation and function via an Akt-dependent but PI3K-independent pathway. These results underscore a novel unrecognized target that regulates DC maturation and function with critical implications in immunopathology at the crossroads of the immune-endocrine circuits. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

Registro:

Documento: Artículo
Título:Nuclear factor (NF)-κB-dependent thyroid hormone receptor β 1 expression controls dendritic cell function via Akt signaling
Autor:Mascanfroni, I.D.; Montesinos, M.D.M.; Alamino, V.A.; Susperreguy, S.; Nicola, J.P.; Ilarregui, J.M.; Masini-Repiso, A.M.; Rabinovich, G.A.; Pellizas, C.G.
Filiación:Centro de Investigaciones en Bioquímica Clínica e Inmunología, Departamento de Bioquímica Clínica, Universidad Nacional de Córdoba, 5000 Córdoba, Argentina
Laboratorio de Inmunopatología, Instituto de Biología Y Medicina Experimental, Consejo Nacional de Investigaciones Científicas Y Técnicas, C1428 Ciudad de Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, C1428 Ciudad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas, Argentina
Fondo para la Investigación Cientifica Y Tecnológica, Argentina
Palabras clave:Adaptive immunity; AKT activation; Akt phosphorylation; Chromatin immunoprecipitation analysis; Dendritic cells; Endocrine systems; Mechanism control; Nuclear factors; Phosphatidylinositol 3-kinase; Promoter region; Small interfering RNA; Thyroid hormone receptor; Thyroid hormones; Activation analysis; Chemical activation; Dendrimers; Endocrinology; Hormones; Phosphorylation; Physiology; RNA; Signaling; Adaptive control systems; immunoglobulin enhancer binding protein; interleukin 12; liothyronine; phosphatidylinositol 3 kinase; protein kinase B; small interfering RNA; thyroid hormone receptor beta; thyroid hormone receptor beta 1; unclassified drug; immunoglobulin enhancer binding protein; interleukin 12; liothyronine; protein kinase B; small interfering RNA; thyroid hormone receptor beta; animal cell; animal tissue; article; autocrine effect; cell function; cell maturation; chromatin immunoprecipitation; controlled study; cytokine production; dendritic cell; enzyme activation; enzyme phosphorylation; female; gene expression regulation; gene function; immunoregulation; mouse; nonhuman; priority journal; promoter region; signal transduction; upregulation; animal; C57BL mouse; immunoblotting; metabolism; phosphorylation; signal transduction; Animals; Dendritic Cells; Enzyme Activation; Female; Gene Expression Regulation; Immunoblotting; Interleukin-12; Mice; Mice, Inbred C57BL; NF-kappa B; Phosphorylation; Proto-Oncogene Proteins c-akt; RNA, Small Interfering; Signal Transduction; Thyroid Hormone Receptors beta; Triiodothyronine
Año:2010
Volumen:285
Número:13
Página de inicio:9569
Página de fin:9582
DOI: http://dx.doi.org/10.1074/jbc.M109.071241
Título revista:Journal of Biological Chemistry
Título revista abreviado:J. Biol. Chem.
ISSN:00219258
CODEN:JBCHA
CAS:interleukin 12, 138415-13-1; liothyronine, 6138-47-2, 6893-02-3; phosphatidylinositol 3 kinase, 115926-52-8; protein kinase B, 148640-14-6; Interleukin-12, 187348-17-0; NF-kappa B; Proto-Oncogene Proteins c-akt, 2.7.11.1; RNA, Small Interfering; Thyroid Hormone Receptors beta; Triiodothyronine, 6893-02-3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219258_v285_n13_p9569_Mascanfroni.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v285_n13_p9569_Mascanfroni

Referencias:

  • Verburg-Van Kemenade, L., Schreck, C., (2007) Gen. Comp. Endocrinol., 152, p. 352
  • Oetting, A., Yen, P.M., (2007) Best Pract. Res. Clin. Endocrinol. Metabol., 21, pp. 193-208
  • Hiroi, Y., Kim, H.H., Ying, H., Furuya, F., Huang, Z., Simoncini, T., Noma, K., Liao, J.K., (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 14104-14109
  • Storey, N.M., Gentile, S., Ullah, H., Russo, A., Muessel, M., Erxleben, C., Armstrong, D.L., (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 5197-5201
  • Davis, P.J., Davis, F.B., Lin, H.Y., (2008) Steroids, 73, pp. 1013-1017
  • Davis, P.J., Leonard, J.L., Davis, F.B., (2008) Front. Neuroendocrinol., 29, pp. 211-218
  • Lei, J., Mariash, C.N., Bhargava, M., Wattenberg, E.V., Ingbar, D.H., (2008) Am. J. Physiol. Lung Cell Mol. Physiol., 294, pp. L749-L754
  • Cao, X., Kambe, F., Moeller, L.C., Refetoff, S., Seo, H., (2005) Mol. Endocrinol., 19, pp. 102-112
  • Brazil, D.P., Yang, Z.Z., Hemmings, B.A., (2004) Trends Biochem. Sci., 29, pp. 233-242
  • Verga Falzacappa, C., Petrucci, E., Patriarca, V., Michienzi, S., Stigliano, A., Brunetti, E., Toscano, V., Misiti, S., (2007) J. Mol. Endocrinol., 38, pp. 221-233
  • Dorshkind, K., Horseman, N.D., (2000) Endocr. Rev., 21, pp. 292-312
  • Klecha, A.J., Genaro, A.M., Gorelik, G., Barreiro Arcos, M.L., Silberman, D.M., Schuman, M., Garcia, S.I., Cremaschi, G.A., (2006) J. Endocrinol., 189, pp. 45-55
  • Guermonprez, P., Valladeau, J., Zitvogel, L., Théry, C., Amigorena, S., (2002) Annu. Rev. Immunol., 20, pp. 621-667
  • Steinman, R.M., Banchereau, J., (2007) Nature, 449, pp. 419-426
  • Bottero, V., Withoff, S., Verma, I.M., (2006) Cell Death Differ., 13, pp. 785-797
  • Zhang, T.Y., Daynes, R.A., (2007) J. Immunol., 178, pp. 2517-2526
  • Mascanfroni, I., Montesinos, M.D.M., Susperreguy, S., Cervi, L., Ilarregui, J.M., Ramseyer, V.D., Masini-Repiso, A.M., Pellizas, C.G., (2008) FASEB J., 22, pp. 1032-1042
  • Inaba, K., Inaba, M., Romani, N., Aya, H., Deguchi, M., Ikehara, S., Muramatsu, S., Steinman, R.M., (1992) J. Exp. Med., 176, pp. 1693-1702
  • Ilarregui, J.M., Croci, D.O., Bianco, G.A., Toscano, M.A., Salatino, M., Vermeulen, M.E., Geffner, J.R., Rabinovich, G.A., (2009) Nat. Immunol., 10, pp. 981-991
  • Straw, A.D., MacDonald, A.S., Denkers, E.Y., Pearce, E.J., (2003) J. Immunol., 170, pp. 727-734
  • Elbashir, S.M., Harborth, J., Weber, K., Tuschl, T., (2002) Methods, 26, pp. 199-213
  • Schreiber, E., Matthias, P., Müller, M.M., Schaffner, W., (1989) Nucleic Acids Res., 17, p. 6419
  • Kuo, M.H., Allis, C.D., (1999) Methods, 19, pp. 425-433
  • Nelson, J.D., Denisenko, O., Bomsztyk, K., (2006) Nat. Protoc., 1, pp. 179-185
  • Nelson, J.D., Denisenko, O., Sova, P., Bomsztyk, K., (2006) Nucleic Acids Res., 34, pp. e2
  • Lam, Q.L., Zheng, B.J., Jin, D.Y., Cao, X., Lu, L., (2007) J. Biol. Chem., 282, pp. 27587-27597
  • Ardeshna, K.M., Pizzey, A.R., Devereux, S., Khwaja, A., (2000) Blood, 96, pp. 1039-1046
  • Kim, K.Y., Baek, A., Hwang, J.E., Choi, Y.A., Jeong, J., Lee, M.S., Cho, D.H., Yang, Y., (2009) Cancer Res., 69, pp. 4018-4026
  • Baumann, C.T., Maruvada, P., Hager, G.L., Yen, P.M., (2001) J. Biol. Chem., 276, pp. 11237-11245
  • Hodin, R.A., Lazar, M.A., Chin, W.W., (1990) J. Clin. Invest., 85, pp. 101-105
  • Wood, W.M., Dowding, J.M., Haugen, B.R., Bright, T.M., Gordon, D.F., Ridgway, E.C., (1994) Mol. Endocrinol., 8, pp. 1605-1617
  • Dhodapkar, M.V., Dhodapkar, K.M., Palucka, A.K., (2008) Cell Death Differ., 15, pp. 39-50
  • Song, G., Ouyang, G., Bao, S., (2005) J. Cell. Mol. Med., 9, pp. 59-71
  • Rane, M.J., Pan, Y., Singh, S., Powell, D.W., Wu, R., Cummins, T., Chen, Q., Klein, J.B., (2003) J. Biol. Chem., 278, pp. 27828-27835
  • Park, D., Lapteva, N., Seethammagari, M., Slawin, K.M., Spencer, D.M., (2006) Nat. Biotechnol., 24, pp. 1581-1590
  • Laderach, D., Compagno, D., Danos, O., Vainchenker, W., Galy, A., (2003) J. Immunol., 171, pp. 1750-1757
  • Guha, M., Mackman, N., (2001) Cell. Signal., 13, pp. 85-94
  • Refetoff, S., Dumitrescu, A.M., (2007) Best Pract. Res. Clin. Endocrinol. Metab., 21, pp. 277-305
  • Bassett, J.H., Harvey, C.B., Williams, G.R., (2003) Mol. Cell. Endocrinol., 213, pp. 1-11
  • Yen, P.M., (2001) Physiol. Rev., 81, pp. 1097-1142
  • Issemann, I., Green, S., (1991) J. Steroid. Biochem. Mol. Biol., 40, pp. 263-269
  • Hoberg, J.E., Popko, A.E., Ramsey, C.S., Mayo, M.W., (2006) Mol. Cell. Biol., 26, pp. 457-471
  • Supakar, P.C., Jung, M.H., Song, C.S., Chatterjee, B., Roy, A.K., (1995) J. Biol. Chem., 270, pp. 837-842

Citas:

---------- APA ----------
Mascanfroni, I.D., Montesinos, M.D.M., Alamino, V.A., Susperreguy, S., Nicola, J.P., Ilarregui, J.M., Masini-Repiso, A.M.,..., Pellizas, C.G. (2010) . Nuclear factor (NF)-κB-dependent thyroid hormone receptor β 1 expression controls dendritic cell function via Akt signaling. Journal of Biological Chemistry, 285(13), 9569-9582.
http://dx.doi.org/10.1074/jbc.M109.071241
---------- CHICAGO ----------
Mascanfroni, I.D., Montesinos, M.D.M., Alamino, V.A., Susperreguy, S., Nicola, J.P., Ilarregui, J.M., et al. "Nuclear factor (NF)-κB-dependent thyroid hormone receptor β 1 expression controls dendritic cell function via Akt signaling" . Journal of Biological Chemistry 285, no. 13 (2010) : 9569-9582.
http://dx.doi.org/10.1074/jbc.M109.071241
---------- MLA ----------
Mascanfroni, I.D., Montesinos, M.D.M., Alamino, V.A., Susperreguy, S., Nicola, J.P., Ilarregui, J.M., et al. "Nuclear factor (NF)-κB-dependent thyroid hormone receptor β 1 expression controls dendritic cell function via Akt signaling" . Journal of Biological Chemistry, vol. 285, no. 13, 2010, pp. 9569-9582.
http://dx.doi.org/10.1074/jbc.M109.071241
---------- VANCOUVER ----------
Mascanfroni, I.D., Montesinos, M.D.M., Alamino, V.A., Susperreguy, S., Nicola, J.P., Ilarregui, J.M., et al. Nuclear factor (NF)-κB-dependent thyroid hormone receptor β 1 expression controls dendritic cell function via Akt signaling. J. Biol. Chem. 2010;285(13):9569-9582.
http://dx.doi.org/10.1074/jbc.M109.071241