Artículo

Encinar, J.A.; Mallo, G.V.; Mizyrycki, C.; Giono, L.; González-Ros, J.M.; Rico, M.; Cánepa, E.; Moreno, S.; Neira, J.L.; Iovanna, J.L. "Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation" (2001) Journal of Biological Chemistry. 276(4):2742-2751
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Abstract:

We have studied the biochemical features, the conformational preferences in solution, and the DNA binding properties of human p8 (hp8), a nucleoprotein whose expression is affected during acute pancreatitis. Biochemical studies show that hp8 has properties of the high mobility group proteins, HMG-I/Y. Structural studies have been carried out by using circular dichroism (near- and far-ultraviolet), Fourier transform infrared, and NMR spectroscopies. All the biophysical probes indicate that hp8 is monomeric (up to 1 mM concentration) and partially unfolded in solution. The protein seems to bind DNA weakly, as shown by electrophoretic gel shift studies. On the other hand, hp8 is a substrate for protein kinase A (PKA). The phosphorylated hp8 (PKAhp8) has a higher content of secondary structure than the nonphosphorylated protein, as concluded by Fourier transform infrared studies. PKAhp8 binds DNA strongly, as shown by the changes in circular dichroism spectra, and gel shift analysis. Thus, although there is not a high sequence homology with HMG-I/Y proteins, hp8 can be considered as a HMG-I/Y-like protein.

Registro:

Documento: Artículo
Título:Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation
Autor:Encinar, J.A.; Mallo, G.V.; Mizyrycki, C.; Giono, L.; González-Ros, J.M.; Rico, M.; Cánepa, E.; Moreno, S.; Neira, J.L.; Iovanna, J.L.
Filiación:Ctro. de Biol. Molecular y Celular, Universidad Miguel Hernández, 03202, Elche, Alicante, Spain
Unit 315 INSERM, 46 Boulevard de la Gaye, 13009 Marseille, France
Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Inst. de Estructura de la Materia, Consejo Sup. de Invest. Cientificas, Serrano 119, 28006 Madrid, Spain
Palabras clave:Biochemistry; DNA; Electrophoresis; Fourier transform infrared spectroscopy; Nuclear magnetic resonance spectroscopy; Gel shift analysis; Proteins; cyclic AMP dependent protein kinase; DNA; high mobility group B1 protein; monomer; nucleoprotein; protein hp8; unclassified drug; basic helix loop helix transcription factor; DNA binding protein; growth promotor; high mobility group A1a protein; high mobility group protein; P8 protein, human; transcription factor; tumor protein; acute pancreatitis; article; binding affinity; biophysics; circular dichroism; gel mobility shift assay; human; infrared spectroscopy; nuclear magnetic resonance spectroscopy; priority journal; protein conformation; protein DNA binding; protein folding; protein phosphorylation; protein secondary structure; sequence analysis; sequence homology; structure analysis; amino acid sequence; comparative study; genetics; metabolism; molecular genetics; nuclear magnetic resonance; nucleotide sequence; phosphorylation; protein binding; protein tertiary structure; Amino Acid Sequence; Basic Helix-Loop-Helix Transcription Factors; Circular Dichroism; Conserved Sequence; Cyclic AMP-Dependent Protein Kinases; DNA-Binding Proteins; Growth Substances; High Mobility Group Proteins; HMGA1a Protein; Humans; Molecular Sequence Data; Neoplasm Proteins; Nuclear Magnetic Resonance, Biomolecular; Phosphorylation; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Spectroscopy, Fourier Transform Infrared; Transcription Factors
Año:2001
Volumen:276
Número:4
Página de inicio:2742
Página de fin:2751
DOI: http://dx.doi.org/10.1074/jbc.M008594200
Handle:http://hdl.handle.net/20.500.12110/paper_00219258_v276_n4_p2742_Encinar
Título revista:Journal of Biological Chemistry
Título revista abreviado:J. Biol. Chem.
ISSN:00219258
CODEN:JBCHA
CAS:DNA, 9007-49-2; Basic Helix-Loop-Helix Transcription Factors; Cyclic AMP-Dependent Protein Kinases, EC 2.7.1.37; DNA-Binding Proteins; Growth Substances; High Mobility Group Proteins; HMGA1a Protein, 124544-67-8; Neoplasm Proteins; P8 protein, human; Transcription Factors
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Citas:

---------- APA ----------
Encinar, J.A., Mallo, G.V., Mizyrycki, C., Giono, L., González-Ros, J.M., Rico, M., Cánepa, E.,..., Iovanna, J.L. (2001) . Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation. Journal of Biological Chemistry, 276(4), 2742-2751.
http://dx.doi.org/10.1074/jbc.M008594200
---------- CHICAGO ----------
Encinar, J.A., Mallo, G.V., Mizyrycki, C., Giono, L., González-Ros, J.M., Rico, M., et al. "Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation" . Journal of Biological Chemistry 276, no. 4 (2001) : 2742-2751.
http://dx.doi.org/10.1074/jbc.M008594200
---------- MLA ----------
Encinar, J.A., Mallo, G.V., Mizyrycki, C., Giono, L., González-Ros, J.M., Rico, M., et al. "Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation" . Journal of Biological Chemistry, vol. 276, no. 4, 2001, pp. 2742-2751.
http://dx.doi.org/10.1074/jbc.M008594200
---------- VANCOUVER ----------
Encinar, J.A., Mallo, G.V., Mizyrycki, C., Giono, L., González-Ros, J.M., Rico, M., et al. Human p8 Is a HMG-I/Y-like Protein with DNA Binding Activity Enhanced by Phosphorylation. J. Biol. Chem. 2001;276(4):2742-2751.
http://dx.doi.org/10.1074/jbc.M008594200