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Abstract:

Although the existence of O-linked oligosaccharide residues in glycoproteins of Plasmodium falciparum has been shown, the existence of N- linked glycoproteins is still a matter of controversy and skepticism. This report demonstrates the unequivocal presence of N-linked glycoproteins in P. falciparum, principally in the ring and young trophozoite stages of the intraerythrocytic cycle. These glycoproteins lose their capacity to bind to concanavalin A-Sepharose after treatment of cultures with tunicamycin under conditions that do not affect protein synthesis. When the glycoproteins were treated with N-Glycanase®, oligosaccharides were released. It was possible to identify an N-linked glycoprotein of >200 kDa in the ring stage and also N-linked glycoproteins in the range of 200-30 kDa in the trophozoite stage. Treatment of trophozoites with 12 μM tunicamycin inhibited differentiation to the schizont stage. To our knowledge, this is the first report in the literature unequivocally showing N-linked glycoproteins in trophozoites of P. falciparum as well as their importance for the differentiation of the intraerythrocytic stages of this parasite.

Registro:

Documento: Artículo
Título:N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum
Autor:Kimura, E.A.; Couto, A.S.; Peres, V.J.; Casal, O.L.; Katzin, A.M.
Filiación:Departamento de Parasitologia, Inst. de Cie. Biomédicas, Universidade de São Paulo, São Paulo, Brazil
Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil
Dept. de Parasitologia, Inst. de Cie. Biomédicas, Universidade de São Paulo, Av. Lineu Prestes 1374, CEP 05508-900, São Paulo SP, Brazil
Palabras clave:asparagine linked oligosaccharide; concanavalin a; glucan synthase; glycoprotein; sepharose; tunicamycin; article; controlled study; erythrocyte; life cycle; nonhuman; plasmodium falciparum; priority journal; protein glycosylation; protein synthesis; schizont; trophozoite; Amidohydrolases; Animals; Carbon Radioisotopes; Chromatography, Affinity; Chromatography, Gel; Chromatography, Paper; Chromatography, Thin Layer; Cycloheximide; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Glucose; Glycoproteins; Humans; Kinetics; Malaria, Falciparum; Mannose; Methionine; Molecular Weight; Oligosaccharides; Parasitemia; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Plasmodium falciparum; Protozoan Proteins; Sulfur Radioisotopes; Tunicamycin
Año:1996
Volumen:271
Número:24
Página de inicio:14452
Página de fin:14461
DOI: http://dx.doi.org/10.1074/jbc.271.24.14452
Título revista:Journal of Biological Chemistry
Título revista abreviado:J. BIOL. CHEM.
ISSN:00219258
CODEN:JBCHA
CAS:Amidohydrolases, EC 3.5.-; Carbon Radioisotopes; Cycloheximide, 66-81-9; Glucose, 50-99-7; Glycoproteins; Mannose, 31103-86-3; Methionine, 63-68-3; Oligosaccharides; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase, EC 3.5.1.52; Protozoan Proteins; Sulfur Radioisotopes; Tunicamycin, 11089-65-9
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219258_v271_n24_p14452_Kimura.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v271_n24_p14452_Kimura

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Citas:

---------- APA ----------
Kimura, E.A., Couto, A.S., Peres, V.J., Casal, O.L. & Katzin, A.M. (1996) . N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum. Journal of Biological Chemistry, 271(24), 14452-14461.
http://dx.doi.org/10.1074/jbc.271.24.14452
---------- CHICAGO ----------
Kimura, E.A., Couto, A.S., Peres, V.J., Casal, O.L., Katzin, A.M. "N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum" . Journal of Biological Chemistry 271, no. 24 (1996) : 14452-14461.
http://dx.doi.org/10.1074/jbc.271.24.14452
---------- MLA ----------
Kimura, E.A., Couto, A.S., Peres, V.J., Casal, O.L., Katzin, A.M. "N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum" . Journal of Biological Chemistry, vol. 271, no. 24, 1996, pp. 14452-14461.
http://dx.doi.org/10.1074/jbc.271.24.14452
---------- VANCOUVER ----------
Kimura, E.A., Couto, A.S., Peres, V.J., Casal, O.L., Katzin, A.M. N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum. J. BIOL. CHEM. 1996;271(24):14452-14461.
http://dx.doi.org/10.1074/jbc.271.24.14452