Artículo
Abstract:
The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.
Registro:
| Documento: | Artículo |
| Título: | Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes |
| Autor: | De Lederkremer, R.M.; Lima, C.; Ramirez, M.I.; Ferguson, M.A.J.; Homans, S.W.; Thomas-Oates, J. |
| Filiación: | Departamento de Quimica Organica, Fac. de Ciencas Exactas y Naturales, Universidad de Buenos Aires, Argentina Department of Biochemistry, University of Dundee, Dundee DD1 4HN, United Kingdom Bijvoet Ctr. for Biomol. Research, Faculty of Chemistry, University of Utrecht, P. O. Box 80083, 3508 TB Utrecht, Netherlands Beit Memorial Research |
| Palabras clave: | glycan; glycan phosphate lipoprotein; unclassified drug; article; chemical structure; epimastigote; nonhuman; priority journal; trypanosoma cruzi; Acylation; Animal; Carbohydrate Sequence; Chromatography, High Pressure Liquid; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Oligosaccharides; Peptidoglycan; Phospholipids; Spectrometry, Mass, Fast Atom Bombardment; Support, Non-U.S. Gov't; Trypanosoma cruzi |
| Año: | 1991 |
| Volumen: | 266 |
| Número: | 35 |
| Página de inicio: | 23670 |
| Página de fin: | 23672 |
| Título revista: | Journal of Biological Chemistry |
| Título revista abreviado: | J. Biol. Chem. |
| ISSN: | 00219258 |
| CODEN: | JBCHA |
| CAS: | lipopeptidophosphoglycan; Oligosaccharides; Peptidoglycan; Phospholipids |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v266_n35_p23670_DeLederkremer |
Citas:
---------- APA ----------
De Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W. & Thomas-Oates, J. (1991) . Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes. Journal of Biological Chemistry, 266(35), 23670-23672.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v266_n35_p23670_DeLederkremer [ ]
---------- CHICAGO ----------
De Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J. "Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes" . Journal of Biological Chemistry 266, no. 35 (1991) : 23670-23672.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v266_n35_p23670_DeLederkremer [ ]
---------- MLA ----------
De Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J. "Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes" . Journal of Biological Chemistry, vol. 266, no. 35, 1991, pp. 23670-23672.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v266_n35_p23670_DeLederkremer [ ]
---------- VANCOUVER ----------
De Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J. Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes. J. Biol. Chem. 1991;266(35):23670-23672.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219258_v266_n35_p23670_DeLederkremer [ ]
