Artículo

Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L. "Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence" (1998) Journal of Bacteriology. 180(7):1607-1617
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Abstract:

Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490- 2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non- gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan biosynthesis reduce the aggressiveness of X. campestris against the plant.

Registro:

Documento: Artículo
Título:Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
Autor:Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
Filiación:Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, UBA, (1405) Buenos Aires, Argentina
Lehrstuhl für Genetik, Universität Bielefeld, D-33501 Bielefeld, Germany
Inst. Invest. Bioquimicas Fund. C., Patricias Argentinas 435, (1405) Buenos Aires, Argentina
Palabras clave:gene product; glucose 1 phosphate; glucuronic acid; lipid; mannose; pentasaccharide; phosphate; protein; transferase; xanthan; article; bacterial virulence; biosynthesis; carboxy terminal sequence; gene; gene function; mutant; nonhuman; nucleotide sequence; priority journal; xanthomonas campestris; Base Sequence; Genes, Bacterial; Molecular Sequence Data; Mutation; Plant Diseases; Polysaccharides, Bacterial; Uridine Diphosphate Glucose; Virulence; Xanthomonas campestris; Bacteria (microorganisms); Negibacteria; Xanthomonas; Xanthomonas campestris pv. campestris
Año:1998
Volumen:180
Número:7
Página de inicio:1607
Página de fin:1617
Handle:http://hdl.handle.net/20.500.12110/paper_00219193_v180_n7_p1607_Katzen
Título revista:Journal of Bacteriology
Título revista abreviado:J. Bacteriol.
ISSN:00219193
CODEN:JOBAA
CAS:Polysaccharides, Bacterial; Uridine Diphosphate Glucose, 133-89-1; xanthan gum, 11138-66-2
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219193_v180_n7_p1607_Katzen.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen

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Citas:

---------- APA ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A. & Ielpi, L. (1998) . Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence. Journal of Bacteriology, 180(7), 1607-1617.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen [ ]
---------- CHICAGO ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. "Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence" . Journal of Bacteriology 180, no. 7 (1998) : 1607-1617.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen [ ]
---------- MLA ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. "Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence" . Journal of Bacteriology, vol. 180, no. 7, 1998, pp. 1607-1617.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen [ ]
---------- VANCOUVER ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence. J. Bacteriol. 1998;180(7):1607-1617.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v180_n7_p1607_Katzen [ ]