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Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating malts which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490- 2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non- gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan biosynthesis reduce the aggressiveness of X. campestris against the plant.


Documento: Artículo
Título:Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence
Autor:Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
Filiación:Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, UBA, (1405) Buenos Aires, Argentina
Lehrstuhl für Genetik, Universität Bielefeld, D-33501 Bielefeld, Germany
Inst. Invest. Bioquimicas Fund. C., Patricias Argentinas 435, (1405) Buenos Aires, Argentina
Palabras clave:gene product; glucose 1 phosphate; glucuronic acid; lipid; mannose; pentasaccharide; phosphate; protein; transferase; xanthan; article; bacterial virulence; biosynthesis; carboxy terminal sequence; gene; gene function; mutant; nonhuman; nucleotide sequence; priority journal; xanthomonas campestris; Base Sequence; Genes, Bacterial; Molecular Sequence Data; Mutation; Plant Diseases; Polysaccharides, Bacterial; Uridine Diphosphate Glucose; Virulence; Xanthomonas campestris; Bacteria (microorganisms); Negibacteria; Xanthomonas; Xanthomonas campestris pv. campestris
Página de inicio:1607
Página de fin:1617
Título revista:Journal of Bacteriology
Título revista abreviado:J. Bacteriol.
CAS:Polysaccharides, Bacterial; Uridine Diphosphate Glucose, 133-89-1; xanthan gum, 11138-66-2


  • Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., Basic local alignment search tool (1990) J. Mol. Biol., 215, pp. 403-410
  • Arial, M., Gough, C.L., Barber, C.E., Boucher, C., Daniels, M.J., Xanthomonas campestris contains a cluster of hrp genes related to the larger hrp cluster of Pseudomonas solanacearum (1991) Mol. Plant-Microbe Interact., 4, pp. 593-601
  • Baird, J., Sandford, P., Cottrell, I., Industrial applications of some new microbial polysaccharides (1983) Bio/Technology, 1, pp. 778-783
  • Baldessari, A., Ielpi, L., Dankert, M.A., A novel galacturonide from Xanthomonas campestris (1990) J. Gen. Microbiol., 136, pp. 1501-1507
  • Barber, C.E., Tang, J.L., Feng, J.X., Pan, M.Q., Wilson, T.J., Slater, H., Dow, J.M., Daniels, M.J., A novel regulatory system required for pathogenicity of Xanthomonas campestris is mediated by a small diffusible signal molecule (1997) Mol. Microbiol., 24, pp. 555-566
  • Barrère, G.C., Barber, C.E., Daniels, M.J., Molecular cloning of genes involved in the production of the extracellular polysaccharide xanthan by Xanthomonas campestris pv. campestris (1986) Int. J. Biol. Macromol., 8, pp. 372-374
  • Battisti, L., Lara, J.C., Leigh, J.A., Specific oligosaccharide form of the Rhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 5625-5629
  • Becker, A., Niehaus, K., Pühler, A., Low-molecular-weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal domain and a missing C-terminal domain (1995) Mol. Microbiol., 16, pp. 191-203
  • Betlach, M.R., Capage, M.A., Doherty, D.H., Hassler, R.A., Henderson, N.M., Vanderslice, R.W., Marrelli, J.D., Ward, M.B., Genetically engineered polymers: Manipulation of xanthan biosynthesis (1987) Industrial Polysaccharides: Genetic Engineering, Structure/ Property Relations and Applications, pp. 35-50. , M. Yalpani (ed.), Elsevier, Amsterdam, The Netherlands
  • Cadmus, M.C., Rogovin, S.P., Burton, K.A., Pittsley, J.E., Knutson, C.A., Jeanes, A., Colonial variation in Xanthomonas campestris NRRL B-1459 and characterization of the polysaccharide from a variant strain (1976) Can. J. Microbiol., 22, pp. 942-948
  • Capage, M.R., Doherty, D.H., Betlach, M.R., Vanderslice, R.W., (1987) Recombinant-DNA Mediated Production of Xanthan Gum, , March International patent WO87/05938
  • Chou, F.L., Chou, H.C., Lin, Y.S., Yang, B.Y., Lin, N.T., Weng, S.F., Tseng, Y.H., The Xanthomonas campestris gumD gene required for synthesis of xanthan gum is involved in normal pigmentation and virulence in causing black rot (1997) Biochem. Biophys. Res. Commun., 233, pp. 265-269
  • Coplin, D.L., Cook, D., Molecular genetics of extracellular polysaccharide biosynthesis in vascular phytopathogenic bacteria (1990) Mol. Plant-Microbe Interact., 3, pp. 271-279
  • Daniels, M.J., Genetics of Xanthomonas (1993) Xanthomonas, pp. 301-339. , J. G. Swings and E. L. Civerolo (ed.), Chapman & Hall, London, England
  • Daniels, M.J., Collinge, D.B., Dow, J.M., Osbourn, A.E., Roberts, I.N., Molecular biology of the interaction of Xanthomonas campestris with plants (1987) Plant Physiol. Biochem., 25, pp. 353-359
  • De Crécy-Lagard, V., Glaser, P., Lejeune, P., Sismeiro, O., Barber, C.E., Daniels, M.J., Danchin, A., A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity (1990) J. Bacteriol., 172, pp. 5877-5883
  • Denny, T.P., Involvement of bacterial polysaccharides in plant pathogenesis (1995) Annu. Rev. Phytopathol., 33, pp. 173-197
  • Ditta, G., Schmidhauser, T., Yakobson, E., Lu, P., Liang, X.W., Finlay, D.R., Guiney, D., Helinski, D.R., Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression (1985) Plasmid, 13, pp. 149-153
  • Geremia, R.A., Petroni, E.A., Ielpi, L., Henrissat, B., Towards a classification of glycosyltransferases based on amino acid sequence similarities: Prokaryotic alpha-mannosyltransferases (1996) Biochem. J., 318, pp. 133-138
  • Glucksmann, M.A., Reuber, T.L., Walker, G.C., Genes needed for the modification, polymerization, export, and processing of succinoglycan by Rhizobium meliloti: A model for succinoglycan biosynthesis (1993) J. Bacteriol., 175, pp. 7045-7055
  • Gonzalez, J.E., Semino, C.E., Walker, G.C., Submitted for publication; Griffin, A.M., Morris, V.J., Gasson, M.J., Identification, cloning and sequencing the aceA gene involved in acetan biosynthesis in Acetobacter xylinum (1996) FEMS Microbiol. Lett., 137, pp. 115-121
  • Harding, N.E., Cleary, J.M., Cabañas, D.K., Rosen, I.G., Kang, K.S., Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris (1987) J. Bacteriol., 169, pp. 2854-2861
  • Harding, N.E., Cleary, J.M., Ielpi, L., Genetics and biochemistry of xanthan gum production by Xanthomonas campestris (1995) Food Biotechnology Microorganism, pp. 495-514. , Y. H. Hui and G. Khachatourians (ed.), VCH Publishers, Inc., New York, N.Y
  • Harding, N.E., Raffo, S., Raimondi, A., Cleary, J.M., Ielpi, L., Identification, genetic and biochemical analysis of genes involved in synthesis sugar nucleotide precursors of xanthan gum (1993) J. Gen. Microbiol., 139, pp. 447-457
  • Hassler, R.A., Doherty, D.H., Genetic engineering of polysaccharide structure: Production of variants of xanthan gum in Xanthomonas campestris (1990) Biotechnol. Prog., 6, pp. 182-187
  • Hötte, B., Rath-Arnold, I., Pühler, A., Simon, R., Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris (1990) J. Bacteriol., 172, pp. 2804-2807
  • Ielmini, M.V., Ielpi, L., Unpublished data; Ielpi, L., Couso, R., Dankert, M., Lipid-linked intermediates in the biosynthesis of xanthan gum (1981) FEBS Lett., 130, pp. 253-256
  • Ielpi, L., Couso, R.O., Dankert, M.A., Pyruvic acid acetal residues are transferred from phosphoenolpyruvate to the pentasaccharide-P-P-lipid. Biochem (1981) Biophys. Res. Commun., 102, pp. 1400-1408
  • Ielpi, L., Couso, R.O., Dankert, M.A., Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris (1993) J. Bacteriol., 175, pp. 2490-2500
  • Ielpi, L., Couso, R.O., Dankert, M.A., Xanthan gum biosynthesis: Acetylation occurs at the prenyl-phospho-sugar stage (1983) Biochem. Int., 6, pp. 323-333
  • Jansson, P.E., Kenne, L., Lindberg, B., Structure of extracellular polysaccharide from Xanthomonas campeslris (1975) Carbohydr. Res., 45, pp. 275-282
  • Katzen, F., Becker, A., Zorreguieta, A., Pühler, A., Ielpi, L., Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide (1996) J. Bacteriol., 178, pp. 4313-4318
  • Kidby, D., Sandford, P., Herman, A., Cadmus, M., Maintenance procedures for the curtailment of genetic instability: Xanthomonas campestris NRRL B-1459 (1977) Appl. Environ. Microbiol., 33, pp. 840-845
  • Köplin, R., Arnold, W., Hötte, B., Simon, R., Wang, G., Pühler, A., Genetics of xanthan production in Xanthomonas campestris: The xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis (1992) J. Bacteriol., 174, pp. 191-199
  • Marzocca, M.P., Harding, N.E., Petroni, E.A., Cleary, J.M., Ielpi, L., Location and cloning of the ketal pyruvate transferase gene of Xanthomonas campestris (1991) J. Bacteriol., 173, pp. 7519-7524
  • Meade, H.M., Long, S.R., Ruvkun, G.B., Brown, S.E., Ausubel, F.M., Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis (1982) J. Bacteriol., 149, pp. 114-122
  • Morrison, D.A., Transformation in Escherichia coli: Cryogenic preservation of competent cells (1977) J. Bacteriol., 132, pp. 349-351
  • Müller, P., Keller, M., Weng, W.M., Quandt, J., Arnold, W., Pühler, A., Genetic analysis of the Rhizobium meliloti exoYFQ operon: ExoY is homologous to sugar transferases and ExoQ represents a transmembrane protein (1993) Mol. Plant-Microbe Interact., 6, pp. 55-65
  • Petroni, E.A., Ielpi, L., Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase gene from Acetobacler xylinum (1996) J. Bacteriol., 178, pp. 4814-4821
  • Priefer, U., Isolation of plasmid DNA (1984) Advanced Molecular Genetics, pp. 14-25. , A. Pühler and K. N. Timmis (ed.), Springer-Verlag KG, Berlin, Germany
  • Reed, J.W., Capage, M., Walker, G.C., Rhizobium meliloti exoG and exoJ mutations affect the ExoX-ExoY system for modulation of exopolysaccharide production (1991) J. Bacteriol., 173, pp. 3776-3788
  • Reuber, T.L., Walker, G.C., Biosynthesis of succinoglycan, a symbolically important exopolysaccharide of Rhizobium meliloti (1993) Cell, 74, pp. 269-280
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
  • Schäfer, A., Tauch, A., Jäger, W., Kalinowski, J., Thierbach, G., Pühler, A., Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum (1994) Gene, 145, pp. 69-73
  • Scott, D.B., Young, C.A., Collins-Emerson, J.M., Terzaghi, E.A., Rockman, E.S., Lewis, P.E., Pankhurst, C.E., Novel and complex chromosomal arrangement of Rhizobium loti nodulation genes (1996) Mol. Plant-Microbe Interact., 9, pp. 187-197
  • Simon, R., High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon (1984) Mol. Gen. Genet., 196, pp. 413-420
  • Simon, R., Priefer, U., Pühler, A., A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria (1983) Bio/Technology, 1, pp. 784-791
  • Stankowski, J., Mueller, B., Zeller, S., Location of a second O-acetyl group in xanthan gum by the reductive-cleavage method (1993) Carbohydr. Res., 241, pp. 321-326
  • Sutherland, I.W., Xanthan (1993) Xanthomonas, pp. 363-388. , J. G. Swings and E. L. Civerolo (ed.), Chapman & Hall, London, England
  • Tang, J.L., Liu, Y.N., Barber, C.E., Dow, J.M., Wootton, J.C., Daniels, M.J., Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris (1991) Mol. Gen. Genet., 226, pp. 409-417
  • Thorne, L., Tansey, L., Pollock, T.J., Clustering of mutations blocking synthesis of xanthan gum by Xanthomonas campestris (1987) J. Bacteriol., 169, pp. 3593-3600
  • Vanderslice, R.W., Doherty, D.H., Capage, M.A., Betlach, M.R., Hassler, R.A., Henderson, N.M., Ryan-Graniero, J., Tecklenburg, M., Genetic engineering of polysaccharide structure in Xanthomonas campestris (1988) Biomedical and Biotechnological Advances in Industrial Polysaccharides, pp. 145-157. , V. Crescenzi, I. C. M. Dea, S. Paoletti, S. S. Stivala, and I. W. Sutherland (ed.), Gordon and Breach Science Publishers, New York, N.Y
  • Wang, L., Liu, D., Reeves, P.R., C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesis (1996) J. Bacteriol., 178, pp. 2598-2604
  • Whitfield, C., Amor, P.A., Köplin, R., Modulation of the surface architecture of gram-negative bacteria by the action of surface polymer:lipid A-core ligase and by determinants of polymer chain length (1997) Mol. Microbiol., 23, pp. 629-638
  • Wunder, D.E., Aaronson, W., Hayes, S.F., Bliss, J.M., Silver, R.P., Nucleotide sequence and mutational analysis of the gene encoding KpsD, a periplasmic protein involved in transport of polysialic acid in Escherichia coli K1 (1994) J. Bacteriol., 176, pp. 4025-4033


---------- APA ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A. & Ielpi, L. (1998) . Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence. Journal of Bacteriology, 180(7), 1607-1617.
Recuperado de [ ]
---------- CHICAGO ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. "Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence" . Journal of Bacteriology 180, no. 7 (1998) : 1607-1617.
Recuperado de [ ]
---------- MLA ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. "Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence" . Journal of Bacteriology, vol. 180, no. 7, 1998, pp. 1607-1617.
Recuperado de [ ]
---------- VANCOUVER ----------
Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Pühler, A., et al. Xanthomonas campestris pv. campestris gum mutants: Effects on xanthan biosynthesis and plant virulence. J. Bacteriol. 1998;180(7):1607-1617.
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