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Abstract:

A genetic locus from Acetobacter xylinum involved in acetan polysaccharide synthesis has been characterized. The chromosomal region was identified by screening a genomic library of A. xylinum in a Xanthomonas campestris mutant defective in xanthan polysaccharide synthesis. The A. xylinum cosmid clone can functionally complement a xanthan-negative mutant. The polymer produced by the recombinant strain was found to be indistinguishable from xanthan. Insertion mutagenesis and subcloning of the cosmid clone combined with complementation studies allowed the identification of a 2.3-kb fragment of A. xylinum chromosomal DNA. The nucleotide sequence of this fragment was analyzed and found to contain an open reading frame (aceA) of 1,182 bp encoding a protein of 43.2 kDa. Results from biochemical and genetic analyses strongly suggest that the aceA gene encodes the GDP- mannose:cellobiosyl-diphosphopolyprenol α-mannosyltransferase enzyme, which is responsible for the transfer of an α-mannosyl residue from GDP-Man to cellobiosyl-diphosphopolyprenol. A search for similarities with other known mannosyltransferases revealed that all bacterial α-mannosyltransferases have a short COOH-termina1 amino acid sequence in common.

Registro:

Documento: Artículo
Título:Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum
Autor:Petroni, E.A.; Ielpi, L.
Filiación:Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1405 Buenos Aires, Argentina
Inst. Invest. Bioquimicas Fund. C., Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Palabras clave:acetylsalicylic acid; bacterial dna; bacterial enzyme; polymer; polysaccharide; transferase; xanthan; acetobacter; article; bacterial gene; carbohydrate synthesis; controlled study; gene isolation; nonhuman; nucleotide sequence; open reading frame; priority journal; xanthomonas; Algorithms; Amino Acid Sequence; Carbohydrate Sequence; Cloning, Molecular; Conjugation, Genetic; Conserved Sequence; Escherichia coli; Gene Library; Genes, Bacterial; Genetic Complementation Test; Gluconacetobacter xylinus; Mannosyltransferases; Molecular Sequence Data; Mutagenesis; Open Reading Frames; Polysaccharides, Bacterial; Recombinant Proteins; Sequence Homology, Amino Acid; Xanthomonas; Acetobacter; Bacteria (microorganisms); Gluconacetobacter xylinus; Xanthomonas; Xanthomonas campestris
Año:1996
Volumen:178
Número:16
Página de inicio:4814
Página de fin:4821
Título revista:Journal of Bacteriology
Título revista abreviado:J. BACTERIOL.
ISSN:00219193
CODEN:JOBAA
CAS:acetan, 110865-71-9; GDP-mannose - cellobiosyl-diphosphopolyprenol mannosyltransferase, EC 2.4.1.-; Mannosyltransferases, EC 2.4.1.-; Polysaccharides, Bacterial; Recombinant Proteins; xanthan gum, 11138-66-2
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Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni

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Citas:

---------- APA ----------
Petroni, E.A. & Ielpi, L. (1996) . Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum. Journal of Bacteriology, 178(16), 4814-4821.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni [ ]
---------- CHICAGO ----------
Petroni, E.A., Ielpi, L. "Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum" . Journal of Bacteriology 178, no. 16 (1996) : 4814-4821.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni [ ]
---------- MLA ----------
Petroni, E.A., Ielpi, L. "Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum" . Journal of Bacteriology, vol. 178, no. 16, 1996, pp. 4814-4821.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni [ ]
---------- VANCOUVER ----------
Petroni, E.A., Ielpi, L. Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl- diphosphopolyprenol α-mannosyltransferase gene from Acetobacter xylinum. J. BACTERIOL. 1996;178(16):4814-4821.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v178_n16_p4814_Petroni [ ]