Artículo
Abstract:
Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed β-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure.
Registro:
| Documento: | Artículo |
| Título: | Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp. |
| Autor: | Altabe, S.; Inon de Iannino, N.; De Mendoza, D.; Ugalde, R.A. |
| Filiación: | Inst. Investigaciones Bioquim., Fac.Cienc. Exactas y Naturales, Universidad de Buenos Aires, Antonio Machado 151, Buenos Aires, Argentina |
| Palabras clave: | restriction endonuclease; article; Azospirillum; gene expression; genetic engineering; membrane; nonhuman; priority journal; Rhizobium radiobacter; sequence homology; virulence; Agrobacterium tumefaciens; Azospirillum; Azospirillum brasilense; Rhizobium; Sinorhizobium meliloti |
| Año: | 1990 |
| Volumen: | 172 |
| Número: | 5 |
| Página de inicio: | 2563 |
| Página de fin: | 2567 |
| DOI: | http://dx.doi.org/10.1128/jb.172.5.2563-2567.1990 |
| Título revista: | Journal of Bacteriology |
| Título revista abreviado: | J. BACTERIOL. |
| ISSN: | 00219193 |
| CODEN: | JOBAA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v172_n5_p2563_Altabe |
Citas:
---------- APA ----------
Altabe, S., Inon de Iannino, N., De Mendoza, D. & Ugalde, R.A. (1990) . Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp. Journal of Bacteriology, 172(5), 2563-2567.http://dx.doi.org/10.1128/jb.172.5.2563-2567.1990
---------- CHICAGO ----------
Altabe, S., Inon de Iannino, N., De Mendoza, D., Ugalde, R.A. "Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp." Journal of Bacteriology 172, no. 5 (1990) : 2563-2567.http://dx.doi.org/10.1128/jb.172.5.2563-2567.1990
---------- MLA ----------
Altabe, S., Inon de Iannino, N., De Mendoza, D., Ugalde, R.A. "Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp." Journal of Bacteriology, vol. 172, no. 5, 1990, pp. 2563-2567.http://dx.doi.org/10.1128/jb.172.5.2563-2567.1990
---------- VANCOUVER ----------
Altabe, S., Inon de Iannino, N., De Mendoza, D., Ugalde, R.A. Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp. J. BACTERIOL. 1990;172(5):2563-2567.http://dx.doi.org/10.1128/jb.172.5.2563-2567.1990
