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Abstract:

Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.

Registro:

Documento: Artículo
Título:Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti
Autor:Ugalde, R.A.; Coira, J.A.; Brill, W.J.
Filiación:Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales and CONICET, Buenos Aires, Argentina
Palabras clave:galactose; galacturonic acid; polysaccharide; uridine diphosphate galactose; uridine diphosphate glucuronic acid; article; nonhuman; priority journal; Sinorhizobium meliloti; Medicago sativa; Rhizobium; Scophthalmus rhombus; Sinorhizobium meliloti
Año:1986
Volumen:168
Número:1
Página de inicio:270
Página de fin:275
DOI: http://dx.doi.org/10.1128/jb.168.1.270-275.1986
Título revista:Journal of Bacteriology
Título revista abreviado:J. BACTERIOL.
ISSN:00219193
CODEN:JOBAA
CAS:galactose, 26566-61-0, 50855-33-9, 59-23-4; galacturonic acid, 14982-50-4, 685-73-4; uridine diphosphate galactose, 2956-16-3; uridine diphosphate glucuronic acid, 2616-64-0
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219193_v168_n1_p270_Ugalde.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v168_n1_p270_Ugalde

Citas:

---------- APA ----------
Ugalde, R.A., Coira, J.A. & Brill, W.J. (1986) . Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti. Journal of Bacteriology, 168(1), 270-275.
http://dx.doi.org/10.1128/jb.168.1.270-275.1986
---------- CHICAGO ----------
Ugalde, R.A., Coira, J.A., Brill, W.J. "Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti" . Journal of Bacteriology 168, no. 1 (1986) : 270-275.
http://dx.doi.org/10.1128/jb.168.1.270-275.1986
---------- MLA ----------
Ugalde, R.A., Coira, J.A., Brill, W.J. "Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti" . Journal of Bacteriology, vol. 168, no. 1, 1986, pp. 270-275.
http://dx.doi.org/10.1128/jb.168.1.270-275.1986
---------- VANCOUVER ----------
Ugalde, R.A., Coira, J.A., Brill, W.J. Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti. J. BACTERIOL. 1986;168(1):270-275.
http://dx.doi.org/10.1128/jb.168.1.270-275.1986