Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R.A. Ugalde, J. Handelsman, and W.J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated glactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.
Documento: | Artículo |
Título: | Biosynthesis of a galactose- and galacturonic acid-containing polysaccharide in Rhizobium meliloti |
Autor: | Ugalde, R.A.; Coira, J.A.; Brill, W.J. |
Filiación: | Instituto de Investigaciones Bioquimicas Fundacion Campomar, Facultad de Ciencias Exactas y Naturales and CONICET, Buenos Aires, Argentina |
Palabras clave: | galactose; galacturonic acid; polysaccharide; uridine diphosphate galactose; uridine diphosphate glucuronic acid; article; nonhuman; priority journal; Sinorhizobium meliloti; Medicago sativa; Rhizobium; Scophthalmus rhombus; Sinorhizobium meliloti |
Año: | 1986 |
Volumen: | 168 |
Número: | 1 |
Página de inicio: | 270 |
Página de fin: | 275 |
DOI: | http://dx.doi.org/10.1128/jb.168.1.270-275.1986 |
Título revista: | Journal of Bacteriology |
Título revista abreviado: | J. BACTERIOL. |
ISSN: | 00219193 |
CODEN: | JOBAA |
CAS: | galactose, 26566-61-0, 50855-33-9, 59-23-4; galacturonic acid, 14982-50-4, 685-73-4; uridine diphosphate galactose, 2956-16-3; uridine diphosphate glucuronic acid, 2616-64-0 |
PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00219193_v168_n1_p270_Ugalde.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219193_v168_n1_p270_Ugalde |