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Abstract:

Chasmagnathus granulatus is a hyper-hyporegulating crab that inhabits changing habitats of salinity in Brazil, Uruguay and Argentina. Since the gills are the main sites for active ion transport in crabs, the adaptive changes in the gill epithelium occurring under different conditions of salinity were studied by means of morphological and morphometric analysis, and immunohistochemical identification of cell proliferation (BrdU technique). In anterior (1-3) gills the epithelium thickness from crabs acclimatised to 12, 34 and 44 g/l ranged from 1.27 to 2.46 μm, with no significant change during acclimatisation, thus denoting a respiratory function. Medial (4-5) gill epithelium was slightly thicker in extreme salinities, but these differences were not statistically significant. In contrast, epithelial thickness of the posterior (6-8) gills increased significantly up to 8.10 μm (dorsal zone of gill 8) both in hyper- and hyposaline media compared with seawater. The dark areas measured in gill 8 treated with AgNO3 revealed putative ion transporting tissue, especially at 12 and 44 g/l, corresponding to the zones of higher epithelial thickness. Hence these areas seem to participate both in hyper- and hyporegulation. Proliferating cells labelled with BrdU almost never occurred in the gills/salinity combinations studied during the initial 48 h of transfer from seawater to hyperconcentrated or diluted media, thus suggesting an increase in cell size rather than cell proliferation.

Registro:

Documento: Artículo
Título:The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells
Autor:Genovese, G.; Luquet, C.M.; Paz, D.A.; Rosa, G.A.; Pellerano, G.N.
Filiación:Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Depto. Cs. Biológicas, FCEyN, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Palabras clave:Crustacea; Ion-regulation; Salinity; ion; sea water; silver nitrate; acclimatization; animal cell; animal tissue; article; cell labeling; cell proliferation; cell size; controlled study; crab; epithelium; gill; immunohistochemistry; ion transport; morphology; morphometrics; nonhuman; priority journal; respiratory function; salinity; technique; thickness; Adaptation, Physiological; Animals; Brachyura; Cell Division; Coloring Agents; Epithelial Cells; Fresh Water; Gills; Ion Transport; Liver; Pancreas; Sodium Chloride; Animalia; Chasmagnathus granulata; Crustacea; Decapoda (Crustacea)
Año:2000
Volumen:197
Número:2
Página de inicio:239
Página de fin:246
DOI: http://dx.doi.org/10.1017/S0021878299006615
Título revista:Journal of Anatomy
Título revista abreviado:J. Anat.
ISSN:00218782
CODEN:JOANA
CAS:Coloring Agents; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218782_v197_n2_p239_Genovese

Referencias:

  • Barra, J.A., Pequeux, A., Humbert, W., A morphological study on gills of a crab acclimated to fresh water (1983) Tissue and Cell, 15, pp. 583-596
  • Burck, H.C., (1969), pp. 51-58. , Tecnica Histologica, Madrid: Paz Montalvo; Burnett, L.E., McMahon, B.R., Gas exchange, hemolymph acid-base status and the role of branchial water stores during air exposure in three littoral crab species (1987) Physiological Zoology, 60 (1), pp. 27-36
  • Compere, P., Wanson, S., Pequeux, A., Gilles, R., Goffinet, G., Ultrastructural changes in the gill epithelium. of the green crab Carcinus maenas in relation to external salinity (1989) Tissue and Cell, 21, pp. 299-318
  • Copeland, D.E., Fine structure of salts and water uptake in the land-crab Gecarcinus lateralis (1968) American Zoologist, 8, pp. 417-432
  • Drach, P., Tchernigovtzeff, C., Sur la methode de determination des stades d'intermue et son application generale aux crustaces (1967) Vie Milieu, 18, pp. 597-607
  • Finol, H.J., Croghan, P.C., Ultrastructure of the branchial epithelium of an amphibious brackish-water crab (1983) Tissue and Cell, 15 (1), pp. 63-75
  • Gilles, R., Pequeux, A., Physiological and ultrastructural studies of NaCl transport in crustacean gills (1986) Bollentino di Zoologia, 53, pp. 173-182
  • Goodman, S.H., Cavey, M.J., Organisation of a phyllobranchiate gill from the green shore crab Carcinus maenas (Crustacea, Decapoda) (1990) Cell and Tissue Research, 260, pp. 495-505
  • Gross, W.J., An analysis of response to osmotic stress in selected decapod Crustacea (1957) Biological Bulletin, 112, pp. 43-62
  • Kikuchi, S., Matsumasa, M., The osmoregulatory tissue around the afferent blood vessels of the coxal gills in the estuarine amphipod, Grandidierella japonica and Melita setiflagella (1993) Tissue and Cell, 25 (4), pp. 627-638
  • Koch, H.J., Essai d'interpretation de la soi-distant 'reduction vitale' de sels d'argent par certains organes d'Arthropodes (1934) Annales de la 'Societe' des Sciences Medicales et Naturelles Bruxelles,, 54, pp. 346-361. , Serie B
  • Lucu, C., Ion transport in the gill epithelium of aquatic Crustacea (1993) Journal of Experimental Zoology, 265, pp. 378-386
  • Luquet, C.M., Ansaldo, M., Acid-base balance and ionic regulation during emersion in the estuarine intertidal crab Chasmagnathus granulata Dana (Decapoda, Grapside) (1997) Comparative Biochemistry and Physiology, 117 A (3), pp. 407-410
  • Luquet, C.M., Ford, P., Rodriguez, E.M., Ansaldo, M., Stella, V., Ionic regulation patterns in two species of estuarine crabs (1992) Comunicaciones Biologicas, 10 (4), pp. 315-325
  • Luquet, C.M., Pellerano, G.N., De Carlo, J.M., Gill morphology and terrestrial adaptation in the estuarine crab Uca uruguayensis (Nobili, 1901) (Decapoda, Brachyura) (1995) Crustaceana, 68 (7), pp. 882-892
  • Luquet, C.M., Pellerano, G., Rosa, G., Salinity-induced changes of the fine structure of the gills of the semiterrestrial estuarine crab Uca uruguayensis (1997) Tissue and Cell, 29 (40), pp. 495-501
  • Luquet, C.M., Rosa, G., Ferrari, C.C., Genovese, G., Pellerano, G.N., Gill morphology of the intertidal estuarine crab Chasmagnathus granulatus Dana, 1851 (Decapoda, Grapsidae) in relation to habitat and respiratory habits (1999) Crustaceana, , in press
  • Mantel, L.H., Farmer, L.L., Osmotic and ionic regulation (1983), pp. 53-161. , In The Biology of Crustacea, 5 (ed. Mantel L.H.), New York: Academic Press; Martelo, M.J., Zanders, I.P., Modifications of gill ultrastructure and ionic composition in the crab Goniopsis cruentata acclimated to various salinities (1986) Comparative Biochemistry and Physiology, 84 A (2), pp. 383-389
  • Martinez, C.B.R., Harris, R.R., Santos, M.C.F., Transepithelial potential differences and sodium fluxes in isolated perfused gills of the mangrove crab Ucides cordatus (1998) Comparative Biochemistry and Physiology, 120 A, pp. 227-236
  • McNamara, J.C., Lima, A.G., The route of ion and water movements across the gill epithelium of the fresh water shrimp Macrobrachium olfersii (Decapoda, Palaemonidae)-Evidence from ultrastructural changes induced by acclimation to saline media (1997) Biological Bulletin, 192 (2), pp. 321-331
  • Mougabure Cueto, G.A., (1998), pp. 1-55. , Interaccion entre la regulacion ionica y el equilibrio acido-base en el cangrejo Chasmagnathus granulata Dana 1851 (Decapoda, Grapsidae). Graduate thesis, Universidad de Buenos Aires; Pequeux, A., Osmotic regulation in crustaceans (1995) Journal of Crustacean Biology, 15, pp. 1-60
  • Pierrot, C., Pequeux, A., Thuet, P., Effects of ions substitutions and of inhibitors on transepithelial potential difference and sodium fluxes in perfused gills of the crab Pachygrapsus marmoratus (1995) Archives of Physiology and Biochemistry, 103, pp. 466-475
  • Rabalais, N.N., Cameron, J.N., Physiological and morphological adaptations of adult Uca subcylindrica to semiarid environments (1985) Biological Bulletin, 168, pp. 135-146
  • Rosa, G.A., Genovese, G., Luquet, C.M., Ansaldo, M., Pellerano, G.N., Histophysiological changes in Chasmagnathus granulatus during acclimation from low to high salinity (1999) Comparative Biochemistry and Physiology, 124 A, pp. S142
  • Santos, E.A., Baldiseroto, B., Bianchini, A., Colares, E.P., Nery, L.E.M., Manzoni, G.C., Respiratory mechanisms and metabolic adaptations of an intertidal crab, Chasmagnathus granulata (Dana, 1851) (1987) Comparative Biochemistry and Physiology, 88 A, pp. 21-25
  • Schubart, C.D., Diesel, R., Osmoregulatory capacities and penetration into terrestrial habitats: A comparative study of Jamaican crabs of the genus Armases Abele, 1992 (Brachyura: Grapsidae: Sesarminae) (1998) Bulletin of Marine Science, 63 (2), pp. 743-752
  • Schubart, C.D., Diesel, R., Osmoregulation and the transition from marine to freshwater and terrestrial life: A comparative study of Jamaican crabs of the genus Sesarma (1999) Archives of Hydrobiology, 145 (3), pp. 331-347
  • Siebers, D., Leweck, K., Markus, H., Winkler, A., Sodium regulation in the shore crab Carcinus maenas as related to ambient salinity (1982) Marine Biology, 36, pp. 37-43
  • Siebers, D., Winkler, A., Lucu, C., Thedens, G., Weichart, D., Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas (1985) Marine Biology, 87, pp. 185-192
  • Taylor, H.H., Taylor, E.W., Gills and lungs: The exchange of gases and ions (1992), pp. 203-293. , In Microscopic Anatomy of Invertebrates, 10 Decapod Crustacea (ed. Harrison FW, Humes AG), New York: Wiley-Liss; Towle, D.W., Sodium transport systems in gills (1990), pp. 241-263. , In Comparative Aspects of Sodium Cotransport Systems, 7 Comparative Physiology (ed. Kinne R.K.H.), Dortmund, Basel: Karger; Van Der Heijden, A.J.H., Verbost, P.M., Eygensteyn, J., Li, J., Wendelaar Bonga, S.E., Flik, G., Mitochondria-rich cells in gills of tilapia (Oreochromis mossambicus) adapted to fresh water or sea water: Quantification by confocal laser scanning microscopy (1997) Journal of Experimental Biology, 200, pp. 55-64
  • Weihrauch, D., Becker, W., Postel, U., Riestenpatt, S., Siebers, D., Active excretion of ammonia across the gills of the shore crab Carcinus maenas and its relation to osmo-regulatory ion uptake (1998) Journal of Comparative Physiology B, 168, pp. 364-376
  • Wendelaar Bonga, S.E., Van Der Meij, J.C.A., Degeneration and death, by apoptosis and necrosis, of the pavement and chloride cells in the gills of the teleost Oreochromis mossambicus (1989) Cell and Tissue Research, 255, pp. 235-243

Citas:

---------- APA ----------
Genovese, G., Luquet, C.M., Paz, D.A., Rosa, G.A. & Pellerano, G.N. (2000) . The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells. Journal of Anatomy, 197(2), 239-246.
http://dx.doi.org/10.1017/S0021878299006615
---------- CHICAGO ----------
Genovese, G., Luquet, C.M., Paz, D.A., Rosa, G.A., Pellerano, G.N. "The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells" . Journal of Anatomy 197, no. 2 (2000) : 239-246.
http://dx.doi.org/10.1017/S0021878299006615
---------- MLA ----------
Genovese, G., Luquet, C.M., Paz, D.A., Rosa, G.A., Pellerano, G.N. "The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells" . Journal of Anatomy, vol. 197, no. 2, 2000, pp. 239-246.
http://dx.doi.org/10.1017/S0021878299006615
---------- VANCOUVER ----------
Genovese, G., Luquet, C.M., Paz, D.A., Rosa, G.A., Pellerano, G.N. The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells. J. Anat. 2000;197(2):239-246.
http://dx.doi.org/10.1017/S0021878299006615