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Abstract:

Conventional carbohydrate histochemistry and the binding patterns of 21 lectins were analysed to characterise the glycoconjugate content in the components of the vomeronasal organ of the armadillo Chaetophractus villosus. The mucomicrovillous complex of the sensory epithelium bound most of the lectins studied. No reaction was observed with Con A, PSA, S-Con A and SBA, and the sustentacular cells were stained with UEA-I, DSL, LEL, STL and Con A. The vomeronasal receptor neurons were labelled with S-WGA, WGA, PNA, UEA-I, STL, Con A, S-Con A, ECL and RCA120. The basal cell layer reacted with S-WGA, WGA, LCA, UEA-I, DSL, LEL, STL, Con A, JAC and VVA. The nonsensory epithelium exhibited a differential staining in relation to the different components. The mucociliary complex stained with ECL, DBA, JAC, RCA120, STL, LCA, PHA-E, PHA-L, LEL, BSL-I and VVA. However, SJA and UEA-I stained the mucus complex lining a subpopulation of columnar cells. The cytoplasm and cell membranes of columnar cells was labelled with DBA, DSL and LCA. The apical region of these cells exhibited moderate reactivity with LEL and SJA. None of the lectins bound specifically to secretory granules of the nonsecretory cells. Basal cells of the nonsensory epithelium were labelled with DSL, LEL, LCA, BSL-I and STL. The vomeronasal glands showed a positive reaction with WGA, DSL, LEL, LCA, DBA, PNA, RCA120 and SBA. Subpopulations of acinar cells were observed with ECL, S-WGA, Con A, S-Con A and DBA. PNA and RCA120 stained the cells lining the glandular ducts. In comparison with previous results obtained in the olfactory mucosa of the same group of armadillos, the carbohydrate composition of the vomeronasal organ sensory epithelium differed from the olfactory sensory epithelium. This is probably related to the different nature of molecules involved in the perireceptor processes.

Registro:

Documento: Artículo
Título:Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)
Autor:Carmanchahi, P.D.; Ferrari, C.C.; Aldana Marcos, H.J.; Affanni, J.M.; Sonez, C.A.; Paz, D.A.
Filiación:Facultad de Medicina, Universidad de Morón, Córdoba, Argentina
Facultad de Agronomia y Veterinaria, Universidad de Rio Cuarto, Córdoba, Argentina
Instituto de Neurociencia, FCEyN, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Palabras clave:Armadillo; Lectins; Mucus; Olfactory mucosa; Vomeronasal organ; concanavalin A; glycoconjugate; lectin; acinar cell; animal cell; armadillo; article; basal cell; carbohydrate analysis; cell membrane; columnar epithelium; cytoplasm; female; histochemistry; lectin binding; male; microvillus; nerve cell; neuroepithelium; nonhuman; olfactory epithelium; priority journal; vomeronasal organ; Animals; Armadillos; Binding Sites; Female; Glycoconjugates; Histocytochemistry; Lectins; Male; Mucous Membrane; Olfactory Mucosa; Vomeronasal Organ; Animalia; Armadillo; Chaetophractus villosus; Dasypodidae; Edentata; Mammalia
Año:2000
Volumen:196
Número:3
Página de inicio:357
Página de fin:370
DOI: http://dx.doi.org/10.1017/S0021878299006123
Título revista:Journal of Anatomy
Título revista abreviado:J. Anat.
ISSN:00218782
CODEN:JOANA
CAS:Glycoconjugates; Lectins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218782_v196_n3_p357_Carmanchahi

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Citas:

---------- APA ----------
Carmanchahi, P.D., Ferrari, C.C., Aldana Marcos, H.J., Affanni, J.M., Sonez, C.A. & Paz, D.A. (2000) . Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra). Journal of Anatomy, 196(3), 357-370.
http://dx.doi.org/10.1017/S0021878299006123
---------- CHICAGO ----------
Carmanchahi, P.D., Ferrari, C.C., Aldana Marcos, H.J., Affanni, J.M., Sonez, C.A., Paz, D.A. "Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)" . Journal of Anatomy 196, no. 3 (2000) : 357-370.
http://dx.doi.org/10.1017/S0021878299006123
---------- MLA ----------
Carmanchahi, P.D., Ferrari, C.C., Aldana Marcos, H.J., Affanni, J.M., Sonez, C.A., Paz, D.A. "Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)" . Journal of Anatomy, vol. 196, no. 3, 2000, pp. 357-370.
http://dx.doi.org/10.1017/S0021878299006123
---------- VANCOUVER ----------
Carmanchahi, P.D., Ferrari, C.C., Aldana Marcos, H.J., Affanni, J.M., Sonez, C.A., Paz, D.A. Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra). J. Anat. 2000;196(3):357-370.
http://dx.doi.org/10.1017/S0021878299006123