Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Conventional histochemistry and the binding patterns of 22 biotinylated lectins were examined for characterisation of glycoconjugates in the components of the olfactory mucosa of the armadillo Chaetophractus villosus. The mucous lining the olfactory epithelium showed binding sites for DSL, WGA, STL, LEL, PHA-E and JAC. Only the basilar processes of the supporting cells stained for Con-A and S-Con A. The olfactory receptor neurons stained with LEL, LCA, Con A, S-Con A, JAC and PNA. The layer of basal cells did not react with any of the lectins studied. Bowman's glands in the lamina propria showed subpopulations of acinar cells reacting with SBA, S-WGA, WGA, STL, Con A, PSA, PNA, SJA, VVA, JAC and S-Con A, but in our optical studies with lectins we were unable to differentiate between mucous and serous cells in the way that is possible on electron microscopy. The ducts of Bowman's glands were labelled with S-WGA, STL, LEL, PHA-E, BSL-I and JAC. This histochemical study on the glycoconjugates of the olfactory mucosa in the order Xenarthra provides a basis for further experimental investigations.


Documento: Artículo
Título:Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus
Autor:Ferrari, C.C.; Carmanchahi, P.D.; Aldana Marcos, H.J.; Mugnaini, M.T.; Affanni, J.M.; Paz, D.A.
Filiación:Instituto de Neurociencia (CONICET), FCEyN, Ciudad Universitaria, Pab. II, 1428 Buenos Aires, Argentina
Palabras clave:Bowman's glands; Histochemistry; Lectins; Olfactory epithelium; Xenarthra; concanavalin a; glycoconjugate; lectin; wheat germ agglutinin; animal tissue; armadillo; article; basal cell; controlled study; female; histochemistry; lamina propria; male; nonhuman; olfactory epithelium; olfactory receptor; priority journal; Acetylglucosamine; Animals; Armadillos; Female; Fucose; Galactose; Glycoconjugates; Histocytochemistry; Lectins; Male; Mannose; Olfactory Mucosa
Página de inicio:395
Página de fin:405
Título revista:Journal of Anatomy
Título revista abreviado:J. Anat.
CAS:Acetylglucosamine, 7512-17-6; Fucose, 3713-31-3; Galactose, 26566-61-0; Glycoconjugates; Lectins; Mannose, 31103-86-3


  • Accili, D., Menghi, G., Bondi, A.M., Scocco, P., Glycoconjugate composition of mammalian parotid glands elucidated in situ by lectins and glycosidases (1992) Acta Histochemica, 92, pp. 196-206
  • Affanni, J.M., Garcia Samartino, L., Observations sur l'activité élétrique du neocortex, du paleocortex, et du bulb olfactif chez chaetophractus villosus (1970) Comptes Rendus, Séances de Société de Biologie, 164 (2), p. 2260
  • Affanni, J.M., Garcia Samartino, L., Comparative study of electrophysiological phenomena in the olfactory bulb of some South American marsupials and edentates (1984) Comparative Physiology of Sensory Systems, pp. 315-331. , (ed. Bolis L, Keynes RD, Madrell SHP), New York: Cambridge University Press
  • Affanni, J.M., Casanave, E.B., Garcia Samartino, L., Ferrari, R., Neocortical and olfactory bulb activity in armadillos submitted to covering with soil (1986) Archives Internationales de Physiologie et Biochemie, 94, pp. 1-279
  • Bal, R.S., Anholt, R.R., Formation of the extracellular mucous matrix of olfactory neuroepithelium: Identification of partially glycosylated and nonglycosylated precursors of olfactomedin (1993) Biochemistry, 32, pp. 1047-1053
  • Benitez, I., Aldana Marcos, H.J., Affanni, J.M., The encephalon of Chaetophractus villosus: A general view of its most salient features (1994) Communicaciones Biológicas, 12, pp. 57-73
  • Burkhardt-Holm, P., Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin (1997) Histochemical Journal, 29, pp. 893-899
  • Chen, Z., Pace, U., Heldeman, J., Shapira, A., Lancet, D., Isolated frog olfactory cilia: A preparation of dendritic membrane from chemosensorial neurons (1986) Journal of Neuroscience, 6, pp. 2146-2154
  • Cook, H.C., Carbohydrates (1990) Theory and Practice of Histological Techniques, pp. 35-64. , (ed. Bancroft JD, Stevens A), New York: Churchill-Livingstone
  • Cuschieri, A., Bannister, L.H., Some histochemical observations on the mucosubstances of the nasal glands in the mouse (1974) Histochemical Journal, 6, pp. 543-558
  • Engelman, G., The phytogeny of the xenarthra (1985) The Ecology and Evolution of Armadillos, Sloth and Vermilinguas, pp. 51-64. , (ed. Montgomery GG), Washington D.C: Smithsonian Institution Press
  • Farbman, A.I., (1992) Cell Biology of Olfaction, p. 282. , New York: Cambridge University Press
  • Ferrari, C.C., Aldana Marcos, J.H., Carmanchahi, P., Benitez, I., Affanni, J.M., The brain of the South American armadillo Dasypus hybridus. A general view of its most salient features (1988) Biocell, 22, pp. 123-140
  • Ferrari, C.C., Aldana Marcos, H.J., Carmanchahi, P., Affanni, J.M., The olfactory mucosa of the South American armadillo Chaetophractus villosus. An ultrastructural study (1998) Anatomical Record, 252, pp. 325-339
  • Ferri, D., Liquori, G.E., Lectin histochemistry of secretory cell glycoconjugates in the nasal mucosa of Podarcis sicula campestri de Betta (Reptilia, Lacertidae) (1989) Basic and Applied Histochemistry, 33, pp. 1907-2207
  • Foster, J.D., Getchell, M.L., Getchell, T.V., Identification of sugar residues in secretory glycoconjugates of olfactory mucosae using the lectin histochemistry (1991) Anatomical Record, 229, pp. 525-544
  • Franceschini, V., Lazzari, M., Revoltella, R.P., Ciani, F., Histochemical study by lectin binding of surface glycoconjugates in the developing olfactory system of the rat (1994) International Journal of Developmental Neuroscience, 12, pp. 197-206
  • Franceschini, V., Ciani, F., Identification of surface glycoconjugates in the olfactory system of turtle (1996) Brain Research, 725, pp. 81-87
  • Frisch, D., Ultrastructure of the mouse olfactory mucosa (1967) American Journal of Anatomy, 121, pp. 87-120
  • Furhrmann, C., Bereiter-Hahn, J., Coincidence of endoplasmic reticulum pattern as visualised by FITC-Con A-fluorescence and electron microscopy (1984) Histochemistry, 80, pp. 153-156
  • Getchell, M.L., Rafols, J.A., Getchell, M.T., Histological and histochemical studies of the secretory components of the salamander olfactory mucosa: Effects of the isoproterenol and olfactory nerve section (1984) Anatomical Record, 208, pp. 553-565
  • Getchell, M.L., Getchell, T.V., Immunohistochemical localization of components of the immune barrier in the olfactory mucosae of salamanders and rats (1991) Anatomical Record, 231, pp. 358-374
  • Gheri Bryk, S., Balboni, G.C., Identification of sugar residues in human fetal olfactory epithelium using lectin histochemistry (1992) Acta Anatomica, 145, pp. 167-174
  • Hempstead, J.L., Morgan, J.I., Fluorescent lectins as cell-specific markers for the rat olfactory epithelium (1983) Chemical Senses, 8, pp. 107-120
  • Hoolbrook, E.H., Mieleszko Szumowski, K.E., Schowb, J.E., An immunohistochemical, ultrastructural and developmental characterisation of the horizontal basal cells of rat olfactory epithelium (1995) Journal of Comparative Neurology, 363, pp. 129-146
  • Ihida, K., Tsuyama, N., Kashio, N., Murata, F., Subcompartment sugar residues of gastric surface mucous cells studied with labelled lectins (1991) Histochemistry, 95, pp. 329-335
  • Jessel, T.M., Hymes, M.A., Dodd, J., Carbohydrates and carbohydrate-binding proteins in the nervous system (1990) Annual Review of Neuroscience, 13, pp. 227-1225
  • Key, B., Giorgi, P., Soybean agglutinin binding to the olfactory system of rat and mouse (1986) Neuroscience Letters, 69, pp. 131-136
  • Kobata, A., Structure and functions of sugar chains of glycoproteins (1992) European Journal of Biochemistry, 209, pp. 483-501
  • Lemaire, C., Orange, N., Saglio, P., Saint, N., Gagnon, J., Moller, G., Characterisation and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (Cyprinus carpio) (1996) European Journal of Biochemistry, 240, pp. 143-149
  • Lev, R., Spicer, S.S., Specific staining of sulphated groups with alcian blue at low pH (1964) Journal of Histochemistry and Cytochemistry, 12, p. 309
  • Lewis, J.L., Dahl, A.R., Olfactory mucosa. Composition, enzymatic localization and metabolism (1995) Handbook of Olfaction and Taste, pp. 32-52. , (ed. Doty R), New York: Marcel Dekker
  • Menco, B.Ph.M., Lectins bind differentially to cilia and microvilli of major and minor cell populations in olfactory and nasal respiratory epithelia (1992) Microscopy Research and Technique, 23, pp. 181-199
  • Menco, B.P., Farbman, A.I., Ultrastructural evidence for multiple mucous domains in frog olfactory epithelium (1992) Cell and Tissue Research, 270, pp. 47-56
  • Menco, B.Ph.M., Birrell, G.B., Fuller, C.M., Ezeh, P.I., Keeton, D.A., Benos, D.J., Ultrastructural localization of amiloride-sensitive sodium channels and Na + , K + -ATPase in the rat's olfactory epithelial surface (1998) Chemical Senses, 23, pp. 137-149
  • Mowry, R.W., Observations of the use of sulphuric ether for the sulphation of hydroxyl groups in the tissue sections (1956) Journal of Histochemistry and Cytochemistry, 4, p. 407
  • Novacek, M.J., The radiation of placental mammals (1994) Major Features of Vertebrate Evolution. Short Courses in Paleontology, 7, pp. 220-237. , London: Paleontological Society
  • Pearse, A.G., (1985) Histochemistry, Theoretical and Applied, p. 439. , New York: Churchill Livingston
  • Pixley, S.K., Farbman, A.I., Menco, B.M., Monoclonal antibody marker for olfactory sustentacular cell microvilli (1997) Anatomical Record, 248, pp. 307-321
  • Saito, H., Ogawa, K., Taniguchi, K., Lectin-binding patterns of the olfactory receptors (olfactory epithelium, vomeronasal organ and septal olfactory organ of masera) in the rat (1994) Experimental Animal, 43, pp. 51-60
  • Shantha, T.R., Nakajima, Y., Histological and histochemical studies on the rhesus monkey (Macaca mulatta) olfactory mucosa (1970) Zeitschrift für Zellforschung, 103, pp. 291-316
  • Shapiro, L.S., Halpern, M., Lectin histochemical identification of carbohydrate moieties in opossum chemosensory system during development, with special emphasis on VVA-identified subdivisions in the accessory olfactory bulb (1995) Journal of Morphology, 224, pp. 331-349. , EE PL
  • Spicer, S.S., Schulte, B.A., Diversity of cell glycoconjugates shown histochemically: A perspective (1992) Journal of Histochemistry and Cytochemistry, 40, pp. 1-38
  • Stewart, W.B., Touloukian, C.E., Lotus lectin labels a subpopulation of olfactory receptors cells (1996) Chemical Senses, 21, pp. 13-18
  • Strous, G.J., Dekker, J., Mucin-type glycoproteins (1992) Critical Reviews in Biochemistry and Molecular Biology, 27, pp. 57-92
  • Zinnin, K., Histochemical studies of the olfactory mucosa of the rabbit (1964) Journal of the Wakayama Medical Society, 15, pp. 213-221


---------- APA ----------
Ferrari, C.C., Carmanchahi, P.D., Aldana Marcos, H.J., Mugnaini, M.T., Affanni, J.M. & Paz, D.A. (1999) . Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus. Journal of Anatomy, 194(3), 395-405.
---------- CHICAGO ----------
Ferrari, C.C., Carmanchahi, P.D., Aldana Marcos, H.J., Mugnaini, M.T., Affanni, J.M., Paz, D.A. "Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus" . Journal of Anatomy 194, no. 3 (1999) : 395-405.
---------- MLA ----------
Ferrari, C.C., Carmanchahi, P.D., Aldana Marcos, H.J., Mugnaini, M.T., Affanni, J.M., Paz, D.A. "Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus" . Journal of Anatomy, vol. 194, no. 3, 1999, pp. 395-405.
---------- VANCOUVER ----------
Ferrari, C.C., Carmanchahi, P.D., Aldana Marcos, H.J., Mugnaini, M.T., Affanni, J.M., Paz, D.A. Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus. J. Anat. 1999;194(3):395-405.