Artículo

Basanta, M.F.; Ponce, N.M.A.; Salum, M.L.; Raffo, M.D.; Vicente, A.R.; Erra-Balsells, R.; Stortz, C.A. "Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening" (2014) Journal of Agricultural and Food Chemistry. 62(51):12418-12427
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Abstract:

Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars ('Chelan', 'Sumele', 'Brooks', 'Sunburst', and 'Regina') with contrasting firmness and cracking susceptibility at two developmental stages (immature and ripe). In contrast to what is usually shown in most fruits, cherry softening could occur is some cultivars without marked increases in water-soluble pectin. Although polyuronide and hemicellulose depolymerization was observed in the water-soluble and dilute-alkali-soluble fractions, only moderate association occurs between initial polymer size and cultivar firmness. In all the genotypes the Na2CO3-soluble polysaccharides (NSF) represented the most abundant and dynamic wall fraction during ripening. Firm cultivars showed upon ripening a lower neutral sugars/uronic acid ratio in the NSF, suggesting that they have a lower proportion of highly branched polyuronides. The similar molar ratios of arabinose plus galactose to rhamnose [(Ara+Gal)/Rha] suggest that the cultivars differed in their relative proportion of homogalacturonan (HG) and rhamnogalacturonan I (RG-I) rather than in the size of the RG side chains; with greater proportions of HG in firmer cherries. Ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was useful to identify the depolymerization patterns of weakly bound pectins, but gave less accurate results on ionically bound pectins, and was unable to fi nd any pattern on covalently bound pectins. © 2014 American Chemical Society.

Registro:

Documento: Artículo
Título:Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening
Autor:Basanta, M.F.; Ponce, N.M.A.; Salum, M.L.; Raffo, M.D.; Vicente, A.R.; Erra-Balsells, R.; Stortz, C.A.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Departamento de Química Orgánica-CIHIDECAR-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Alto Valle de Río Negro, Ruta Nac. 22, Km. 1190, Allen, 8332, Argentina
Centro de Investigación Y Desarrollo en Criotecnología de Alimentos (CIDCA), Laboratorio de Investigación en Productos Agroindustriales (LIPA), Facultad de Ciencias Agrarias y Forestales, Calle 60 y 119, CONICET-UNLP, La Plata, 1900, Argentina
Palabras clave:Development; Hemicellulose; MALDI-TOF; Pectin; Softening; Cellulose; Desorption; Fruits; Development; Hemicellulose; MALDI-TOF; Pectin; Softening; Mass spectrometry; Ara; Prunus avium; Pseudobahia; polysaccharide; cell wall; chemistry; classification; fruit; growth, development and aging; metabolism; Prunus; Cell Wall; Fruit; Polysaccharides; Prunus
Año:2014
Volumen:62
Número:51
Página de inicio:12418
Página de fin:12427
DOI: http://dx.doi.org/10.1021/jf504357u
Título revista:Journal of Agricultural and Food Chemistry
Título revista abreviado:J. Agric. Food Chem.
ISSN:00218561
CODEN:JAFCA
CAS:Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218561_v62_n51_p12418_Basanta

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Citas:

---------- APA ----------
Basanta, M.F., Ponce, N.M.A., Salum, M.L., Raffo, M.D., Vicente, A.R., Erra-Balsells, R. & Stortz, C.A. (2014) . Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening. Journal of Agricultural and Food Chemistry, 62(51), 12418-12427.
http://dx.doi.org/10.1021/jf504357u
---------- CHICAGO ----------
Basanta, M.F., Ponce, N.M.A., Salum, M.L., Raffo, M.D., Vicente, A.R., Erra-Balsells, R., et al. "Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening" . Journal of Agricultural and Food Chemistry 62, no. 51 (2014) : 12418-12427.
http://dx.doi.org/10.1021/jf504357u
---------- MLA ----------
Basanta, M.F., Ponce, N.M.A., Salum, M.L., Raffo, M.D., Vicente, A.R., Erra-Balsells, R., et al. "Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening" . Journal of Agricultural and Food Chemistry, vol. 62, no. 51, 2014, pp. 12418-12427.
http://dx.doi.org/10.1021/jf504357u
---------- VANCOUVER ----------
Basanta, M.F., Ponce, N.M.A., Salum, M.L., Raffo, M.D., Vicente, A.R., Erra-Balsells, R., et al. Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening. J. Agric. Food Chem. 2014;62(51):12418-12427.
http://dx.doi.org/10.1021/jf504357u