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Abstract:

There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. © 2011 American Chemical Society.

Registro:

Documento: Artículo
Título:Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
Autor:Cenzano, A.M.; Cantoro, R.; Hernández-Sotomayor, S.M.T.; Abdala, G.I.; Racagni, G.E.
Filiación:Unidad de Investigación Ecología Terrestre, Centro Nacional Patagónico (CENPAT-CONICET), Boulevard Brown 2915, 9120 Puerto Madryn, Chubut, Argentina
Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas A la Agricultura, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE Buenos Aires, Argentina
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Calle 43 No. 130 Col. Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina
Idioma: Inglés
Palabras clave:electrospray ionization tandem mass spectrometry; galactolipid; kinase; phospholipid; potato; Solanum tuberosum; Electrospray ionization tandem mass spectrometry; galactolipid; kinase; potato; Solanum tuberosum; Biochemistry; Enzymes; Fatty acids; Glycerol; Ionization; Mass spectrometry; Metabolism; Phospholipids; Phosphorylation; Electrospray ionization; phosphotransferase; vegetable protein; article; chemistry; electrospray mass spectrometry; enzymology; evaluation; growth, development and aging; lipid metabolism; metabolism; methodology; phosphorylation; plant tuber; potato; Lipid Metabolism; Phosphorylation; Phosphotransferases; Plant Proteins; Plant Tubers; Solanum tuberosum; Spectrometry, Mass, Electrospray Ionization; Solanum tuberosum
Año:2012
Volumen:60
Número:1
Página de inicio:418
Página de fin:426
DOI: http://dx.doi.org/10.1021/jf204269y
Título revista:Journal of Agricultural and Food Chemistry
Título revista abreviado:J. Agric. Food Chem.
ISSN:00218561
CODEN:JAFCA
CAS:phosphotransferase, 9031-09-8, 9031-44-1; Phosphotransferases, 2.7.-; Plant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00218561_v60_n1_p418_Cenzano

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Citas:

---------- APA ----------
Cenzano, A.M., Cantoro, R., Hernández-Sotomayor, S.M.T., Abdala, G.I. & Racagni, G.E. (2012) . Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons. Journal of Agricultural and Food Chemistry, 60(1), 418-426.
http://dx.doi.org/10.1021/jf204269y
---------- CHICAGO ----------
Cenzano, A.M., Cantoro, R., Hernández-Sotomayor, S.M.T., Abdala, G.I., Racagni, G.E. "Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons" . Journal of Agricultural and Food Chemistry 60, no. 1 (2012) : 418-426.
http://dx.doi.org/10.1021/jf204269y
---------- MLA ----------
Cenzano, A.M., Cantoro, R., Hernández-Sotomayor, S.M.T., Abdala, G.I., Racagni, G.E. "Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons" . Journal of Agricultural and Food Chemistry, vol. 60, no. 1, 2012, pp. 418-426.
http://dx.doi.org/10.1021/jf204269y
---------- VANCOUVER ----------
Cenzano, A.M., Cantoro, R., Hernández-Sotomayor, S.M.T., Abdala, G.I., Racagni, G.E. Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons. J. Agric. Food Chem. 2012;60(1):418-426.
http://dx.doi.org/10.1021/jf204269y