Echinococcus granulosus sensu stricto (s.s.) is the major cause of human cystic echinococcosis worldwide and is listed among the most severe parasitic diseases of humans. To date, numerous studies have investigated the genetic diversity and population structure of E. granulosus s.s. in various geographic regions. However, there has been no global study. Recently, using mitochondrial DNA, it was shown that E. granulosus s.s. G1 and G3 are distinct genotypes, but a larger dataset is required to confirm the distinction of these genotypes. The objectives of this study were to: (i) investigate the distinction of genotypes G1 and G3 using a large global dataset; and (ii) analyse the genetic diversity and phylogeography of genotype G1 on a global scale using near-complete mitogenome sequences. For this study, 222 globally distributed E. granulosus s.s. samples were used, of which 212 belonged to genotype G1 and 10 to G3. Using a total sequence length of 11,682 bp, we inferred phylogenetic networks for three datasets: E. granulosus s.s. (n = 222), G1 (n = 212) and human G1 samples (n = 41). In addition, the Bayesian phylogenetic and phylogeographic analyses were performed. The latter yielded several strongly supported diffusion routes of genotype G1 originating from Turkey, Tunisia and Argentina. We conclude that: (i) using a considerably larger dataset than employed previously, E. granulosus s.s. G1 and G3 are indeed distinct mitochondrial genotypes; (ii) the genetic diversity of E. granulosus s.s. G1 is high globally, with lower values in South America; and (iii) the complex phylogeographic patterns emerging from the phylogenetic and geographic analyses suggest that the current distribution of genotype G1 has been shaped by intensive animal trade. © 2018 Australian Society for Parasitology
Documento: | Artículo |
Título: | Global phylogeography and genetic diversity of the zoonotic tapeworm Echinococcus granulosus sensu stricto genotype G1 |
Autor: | Kinkar, L.; Laurimäe, T.; Acosta-Jamett, G.; Andresiuk, V.; Balkaya, I.; Casulli, A.; Gasser, R.B.; van der Giessen, J.; González, L.M.; Haag, K.L.; Zait, H.; Irshadullah, M.; Jabbar, A.; Jenkins, D.J.; Kia, E.B.; Manfredi, M.T.; Mirhendi, H.; M'rad, S.; Rostami-Nejad, M.; Oudni-M'rad, M.; Pierangeli, N.B.; Ponce-Gordo, F.; Rehbein, S.; Sharbatkhori, M.; Simsek, S.; Soriano, S.V.; Sprong, H.; Šnábel, V.; Umhang, G.; Varcasia, A.; Saarma, U. |
Filiación: | Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, Tartu, 51003, Estonia Instituto de Medicina Preventiva Veterinaria y Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile Laboratorio de Zoonosis Parasitarias, FCEyN, UNMdP, Funes 3350, CP: 7600 Mar del Plata, Buenos Aires, Argentina Department of Parasitology, Faculty of Veterinary Medicine, University of Atatürk, Erzurum, Turkey World Health Organization Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis, European Union Reference Laboratory for Parasites (EURLP), Istituto Superiore di Sanità, Viale Regina Elena 299, Rome, 00161, Italy Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia Centre for Infectious Disease Control Netherlands, National Institute for Public Health and Environment, P.O. Box 1, Bilthoven, BA 3720, Netherlands Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, 28220, Spain Departamento de Genética, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil Parasitology and Mycology Department, Mustapha University Hospital, Algiers, 16000, Algeria Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh, 202002, India School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, NSW 2678, Australia Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Veterinary Medicine, Università degli Studi di Milano, via Celoria 10, Milan, 20133, Italy Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Laboratory of Medical and Molecular Parasitology-Mycology (LP3M), LR 12ES08. Faculty of Pharmacy, University of Monastir, Monastir, 5000, Tunisia Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Microbiology and Parasitology, Faculty of Medical Sciences, Comahue National University, Buenos Aires 1400, Neuquén, 8300, Argentina Department of Parasitology, Faculty of Pharmacy, Complutense University, Plaza Ramón y Cajal s/n, Madrid, 28040, Spain Merial GmbH, Kathrinenhof Research Center, Walchenseestr. 8-12, Rohrdorf, 83101, Germany Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, 23119, Turkey Institute of Parasitology, Slovak Academy of Sciences, Košice, Hlinkova 3, Košice, 040 01, Slovakia ANSES, Nancy Laboratory for Rabies and Wildlife, Wildlife Surveillance and Eco-epidemiology Unit, Malzéville, 54220, France Laboratory of Parasitology, Veterinary Teaching Hospital, Department of Veterinary Medicine, University of Sassari, Via Vienna, Sassari, 2-07100, Italy |
Palabras clave: | Cystic echinococcosis; Echinococcus granulosus; Genetic variability; Global phylogeography; Livestock domestication; Mitochondrial genome; mitochondrial DNA; domestication; flatworm; genetic variation; genome; genotype; livestock; mitochondrion; phylogeography; Argentina; Article; Bayes theorem; Echinococcus granulosus; gene sequence; genetic variability; genotype; human; microbial diversity; nonhuman; phylogeny; phylogeography; Tunisia; Turkey (republic); Animalia; Echinococcus granulosus |
Año: | 2018 |
Volumen: | 48 |
Número: | 9-10 |
Página de inicio: | 729 |
Página de fin: | 742 |
DOI: | http://dx.doi.org/10.1016/j.ijpara.2018.03.006 |
Título revista: | International Journal for Parasitology |
Título revista abreviado: | Int. J. Parasitol. |
ISSN: | 00207519 |
CODEN: | IJPYB |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v48_n9-10_p729_Kinkar |