Artículo

Krivokapich, S.J.; Pozio, E.; Gatti, G.M.; Gonzalez Prous, C.L.; Ribicich, M.; Marucci, G.; La Rosa, G.; Confalonieri, V. "Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America" (2012) International Journal for Parasitology. 42(10):903-910
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Abstract:

Until a few years ago, Trichinella spiralis was the only taxon of the genus Trichinella detected in both domestic and wild animals of South America. Recently, a new genotype, named Trichinella T12, was identified in cougars (Puma concolor) from Argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. In the present study, cross-breeding experiments indicated that Trichinella T12 is reproductively isolated from all other encapsulated Trichinella spp. and suggested that it is biologically more similar to Trichinella britovi and Trichinella murrelli than to the other encapsulated species/genotypes. Biological assays revealed that the reproductive capacity index of Trichinella T12 was ∼4 and >2000 times lower than those of T. spiralis in mice and rats, respectively. The reproductive capacity index of Trichinella T12 in domestic pigs ranged from 0.0 to 0.05. Larvae parasitising the muscles of carnivores were infective to mice after freezing at -5 °C for 3. months, but they lost infectivity after freezing at -18 °C for 1. week. The region within the rDNA, known as the expansion segment V, showed a unique sequence which differs from those of all other known Trichinella spp./genotypes. The biological, geographical and molecular data support the classification of the genotype Trichinella T12 as a new species widespread in the Neotropical region, for which we propose the name Trichinella patagoniensis n. sp. © 2012 Australian Society for Parasitology Inc.

Registro:

Documento: Artículo
Título:Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America
Autor:Krivokapich, S.J.; Pozio, E.; Gatti, G.M.; Gonzalez Prous, C.L.; Ribicich, M.; Marucci, G.; La Rosa, G.; Confalonieri, V.
Filiación:Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas Malbrán, Administración Nacional de Laboratorios e Institutos de Salud, Dr. Carlos G., Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
Cátedra de Parasitología y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Av. Chorroarín 280, C1427CWO Buenos Aires, Argentina
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos AiresCiudad Universitaria, Pab. II, Intendente Guiraldes y Costanera Norte, 1428 Buenos Aires, Argentina
Palabras clave:Argentina; Nematoda; New species; Puma concolor; Ribosomal DNA; Taxonomy; Trichinella patagoniensis; cytochrome c oxidase; cytochrome c oxidase 1; genomic DNA; ribosome DNA; RNA 5S; spacer DNA; unclassified drug; carnivore; DNA; mammal; nematode; Neotropical Region; new species; taxonomy; animal experiment; animal model; animal tissue; article; bioassay; carnivore; cat; controlled study; cross breeding; cytochrome c oxidase 1 gene; DNA sequence; domestic pig; experimental infection; female; gene; genetic variability; genotype; in vitro study; male; mouse; nonhuman; nucleotide sequence; phylogeny; polymerase chain reaction; puma; rat; reproductive isolation; sequence alignment; sequence analysis; South America; Trichinella; Trichinella patagoniensis; trichinosis; Animals; Argentina; Female; Genetic Speciation; Larva; Male; Mice; Molecular Sequence Data; Phylogeny; Puma; Rats; Rats, Wistar; Swine; Swine Diseases; Trichinella; Trichinellosis; Argentina; Animalia; Mammalia; Mus; Nematoda; Puma concolor; Rattus; Sus scrofa domestica; Trichinella; Trichinella britovi; Trichinella murrelli; Trichinella spiralis
Año:2012
Volumen:42
Número:10
Página de inicio:903
Página de fin:910
DOI: http://dx.doi.org/10.1016/j.ijpara.2012.07.009
Título revista:International Journal for Parasitology
Título revista abreviado:Int. J. Parasitol.
ISSN:00207519
CODEN:IJPYB
CAS:cytochrome c oxidase, 72841-18-0, 9001-16-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v42_n10_p903_Krivokapich

Referencias:

  • Britov, V.A., Trichinellosis problem in the Primorsk region (1995) Vet. Sci. Res. Inst., pp. 1-51. , (Far East, Primorsk branch, Vladivostok, in Russian)
  • Culver, M., Johnson, W.E., Pecon-Slattery, J., O'Brien, S.J., Genomic ancestry of the American puma (Puma concolor) (2000) J. Hered., 91, pp. 186-197
  • Dame, J.B., Murrell, K.D., Worley, D.E., Schad, G.A., Trichinella spiralis: genetic evidence for synanthropic subspecies in sylvatic hosts (1987) Exp. Parasitol., 64, pp. 195-203
  • Dick, T.A., Species and intraspecific variation (1983) Trichinella and Trichinosis, pp. 31-73. , Plenum Press, New York, W.C. Campbell (Ed.)
  • Dworkin, M.S., Gamble, H.R., Zarlenga, D.S., Tennican, P.O., Outbreak of trichinellosis associated with eating cougar jerky (1996) J. Infect. Dis., 174, pp. 663-666
  • Gamble, H.R., Bessonov, A.S., Cuperlovic, K., Gajadhar, A.A., van Knapen, F., Noeckler, K., Schenone, H., Zhu, X., International commission on trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption (2000) Vet. Parasitol., 93, pp. 393-408
  • Kapel, C.M., Gamble, H.R., Infectivity, persistence, and antibody response to domestic and sylvatic Trichinella spp. in experimentally infected pigs (2000) Int. J. Parasitol., 30, pp. 215-221
  • Krivokapich, S.J., Molina, V., Bergagna, H.F.J., Guarnera, E.A., Epidemiological survey of Trichinella infection in domestic, synanthropic and sylvatic animals from Argentina (2006) J. Helminthol., 80, pp. 267-269
  • Krivokapich, S.J., Prous, C.L., Gatti, G.M., Confalonieri, V., Molina, V., Matarasso, H., Guarnera, E., Molecular evidence for a novel encapsulated genotype of Trichinella from Patagonia, Argentina (2008) Vet. Parasitol., 156, pp. 234-240
  • La Rosa, G., Marucci, G., Rosenthal, B.M., Pozio, E., Development of a single larva microsatellite analysis to investigate the population structure of Trichinella spiralis (2012) Infect. Genet. Evol., 12, pp. 369-376
  • Murrell, K.D., Lichtenfels, R.J., Zarlenga, D.S., Pozio, E., The systematics of the genus Trichinella with a key to species (2000) Vet. Parasitol., 93, pp. 293-307
  • Nagano, I., Wu, Z., Matsuo, A., Pozio, E., Takahashi, Y., Identification of Trichinella isolates by polymerase chain reaction-restriction fragment length polymorphism of the mitochondrial cytochrome c-oxidase subunit I gene (1999) Int. J. Parasitol., 29, pp. 1113-1120
  • Nöckler, K., Serrano, F.J., Boireau, P., Kapel, C.M., Pozio, E., Experimental studies in pigs on Trichinella detection in different diagnostic matrices (2005) Vet. Parasitol., 132, pp. 85-90
  • Pozio, E., La Rosa, G., Murrell, K.D., Lichtenfels, J.R., Taxonomic revision of the genus Trichinella (1992) J. Parasitol., 78, pp. 654-659
  • Pozio, E., La Rosa, G., Rossi, P., Murrell, K.D., Biological characterization of Trichinella isolates from various host species and geographical regions (1992) J. Parasitol., 78, pp. 647-653
  • Pozio, E., Owen, I.L., La Rosa, G., Sacchi, L., Rossi, P., Corona, S., Trichinella papuae n.sp. (Nematoda), a new non-encapsulated species from domestic and sylvatic swine of Papua New Guinea (1999) Int. J. Parasitol., 29, pp. 1825-1839
  • Pozio, E., Factors affecting the flow among domestic synanthropic and sylvatic cycles of Trichinella (2000) Vet. Parasitol., 93, pp. 241-262
  • Pozio, E., La Rosa, G., Trichinella murrelli n. sp: etiological agent of sylvatic trichinellosis in temperate areas of North America (2000) J. Parasitol., 86, pp. 134-139
  • Pozio, E., Murrell, K.D., Systematics and epidemiology of Trichinella (2006) Adv. Parasitol., 63, pp. 367-439
  • Pozio, E., Taxonomy, biology and epidemiology of Trichinella parasites (2007) FAO/WHO/OIE guidelines for the surveillance, management, prevention and control of trichinellosis, pp. 1-35. , World Organisation for Animal Health Press, Paris, France, J. Dupouy-Camet, K.D. Murrell (Eds.)
  • Pozio, E., Hoberg, E., La Rosa, G., Zarlenga, D.S., Molecular taxonomy, phylogeny and biogeography of nematodes belonging to the Trichinella genus (2009) Infect. Genet. Evol., 9, pp. 606-616
  • Pozio, E., La Rosa, G., Trichinella (2010) Molecular Detection of Foodborne Pathogens, pp. 851-863. , CRC Press, Taylor and Francis Group, Boca Raton, FL, USA, L. Dongyou (Ed.)
  • Ribicich, M., Gamble, H.R., Bolpe, J., Scialfa, E., Krivokapich, S., Cardillo, N., Betti, A., Rosa, A., Trichinella infection in wild animals from endemic regions of Argentina (2010) Parasitol. Res., 107, pp. 377-380
  • Rombout, S., Bosch, J.W.B., van Der Giessen, J., Detection and identification of eight Trichinella genotypes by reverse line blot hybridization (2001) J. Clin. Microbiol., 39, pp. 642-646
  • Rosenthal, B.M., La Rosa, G., Zarlenga, D., Dunams, D., Chunyu, Y., Mingyuan, L., Pozio, E., Human dispersal of Trichinella spiralis in domesticated pigs (2008) Infect. Genet. Evol., 8, pp. 799-805
  • Tamura, K., Dudley, J., Nei, M., Kumar, S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 (2007) Mol. Biol. Evol., 24, pp. 1596-1599
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res., 22, pp. 4673-4680
  • Zarlenga, D.S., Chute, M.B., Martin, A., Kapel, C.M., A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella (1999) Int. J. Parasitol., 29, pp. 1859-1867
  • Zarlenga, D.S., Rosenthal, B.M., La Rosa, G., Pozio, E., Hoberg, E.P., Post-Miocene expansion, colonization, and host switching drove speciation among extant nematodes of the archaic genus Trichinella (2006) Proc. Natl. Acad. Sci. USA, 103, pp. 7354-7359

Citas:

---------- APA ----------
Krivokapich, S.J., Pozio, E., Gatti, G.M., Gonzalez Prous, C.L., Ribicich, M., Marucci, G., La Rosa, G.,..., Confalonieri, V. (2012) . Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America. International Journal for Parasitology, 42(10), 903-910.
http://dx.doi.org/10.1016/j.ijpara.2012.07.009
---------- CHICAGO ----------
Krivokapich, S.J., Pozio, E., Gatti, G.M., Gonzalez Prous, C.L., Ribicich, M., Marucci, G., et al. "Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America" . International Journal for Parasitology 42, no. 10 (2012) : 903-910.
http://dx.doi.org/10.1016/j.ijpara.2012.07.009
---------- MLA ----------
Krivokapich, S.J., Pozio, E., Gatti, G.M., Gonzalez Prous, C.L., Ribicich, M., Marucci, G., et al. "Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America" . International Journal for Parasitology, vol. 42, no. 10, 2012, pp. 903-910.
http://dx.doi.org/10.1016/j.ijpara.2012.07.009
---------- VANCOUVER ----------
Krivokapich, S.J., Pozio, E., Gatti, G.M., Gonzalez Prous, C.L., Ribicich, M., Marucci, G., et al. Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America. Int. J. Parasitol. 2012;42(10):903-910.
http://dx.doi.org/10.1016/j.ijpara.2012.07.009