Abstract:
The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motif proposed by Herrera et al. (2007) to define four haplotypes in Colombia, we could classify these genotypes into four distinct Tc I SL-IR groups, three corresponding to the former haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and one novel group, Ie (19 genotypes). Genotypes harbouring the Tc Ic motif were not detected in our study. Tc Ia was associated with domestic cycles in southern and northern South America and sylvatic cycles in Central and North America. Tc Ib was found in all transmission cycles from Colombia. Tc Id was identified in all transmission cycles from Argentina and Colombia, including Chagas cardiomyopathy patients, sylvatic Brazilian samples and human cases from French Guiana, Panama and Venezuela. Tc Ie gathered five samples from domestic Triatoma infestans from northern Argentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and two chagasic patients from Chile and one from a Bolivian patient with chagasic reactivation. Mixed infections by Tc Ia. +. Tc Id, Tc Ia. +. Tc Ie and Tc Id. +. Tc Ie were detected in vector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Id were identified in different tissues from a heart transplanted Chagas cardiomyopathy patient with reactivation, denoting histotropism. Trypanosoma cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphis virginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodnius neglectus from Brazil and M. spinolai and M. gajardoi from Chile are to our knowledge described for the first time. © Australian Society for Parasitology Inc.
Registro:
Documento: |
Artículo
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Título: | Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
Autor: | Cura, C.I.; Mejía-Jaramillo, A.M.; Duffy, T.; Burgos, J.M.; Rodriguero, M.; Cardinal, M.V.; Kjos, S.; Gurgel-Gonçalves, R.; Blanchet, D.; De Pablos, L.M.; Tomasini, N.; da Silva, A.; Russomando, G.; Cuba, C.A.C.; Aznar, C.; Abate, T.; Levin, M.J.; Osuna, A.; Gürtler, R.E.; Diosque, P.; Solari, A.; Triana-Chávez, O.; Schijman, A.G. |
Filiación: | Lab. de Biología Molecular de la Enfermedad de Chagas, INGEBI, CONICET, Buenos Aires, Argentina Grupo Biología y Control de Enfermedades Infecciosas (BCEI), Universidad de Antioquia, Medellín, Colombia Lab. de Genética Evolutiva, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Lab. de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Department of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, United States Parasitologia Médica e Biologia de Vetores, Área de Patologia, Faculdade de Medicina, Universidade de Brasilia, Brasilia DF, Brazil Laboratoire Hospitalier et Universitaire de Parasitologie Mycologie, EA 3593, Faculté de Médecine, Université des Antilles et de la Guyane, Cayenne, French Guiana Institute of Biotechnology, Molecular Parasitology Group, University of Granada, Granada, Spain Laboratorio de Patología Experimental, IPE, Universidad Nacional de Salta, Salta, Argentina Departamento de Biología Molecular, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Asunción, Paraguay Sección de Biología Molecular, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile
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Palabras clave: | Chagas disease; Discrete typing unit; Genotype; PCR; Spliced-leader intergenic region; Trypanosoma cruzi I; spacer DNA; Chagas disease; detection method; disease transmission; feces; genotype; geographical region; polymerase chain reaction; Argentina; article; Bolivia; Brazil; Central America; Chagas disease; Chile; Colombia; controlled study; DNA polymorphism; DNA splicing; endemic disease; feces analysis; gene identification; gene sequence; genotype; geographic distribution; haplotype; human; life cycle; microsatellite marker; nonhuman; North America; nucleotide sequence; opossum; Paraguay; parasite transmission; parasite vector; polymerase chain reaction; protein motif; sequence alignment; sequence analysis; South America; Triatoma infestans; Trypanosoma cruzi; United States; Animals; Base Sequence; Chagas Disease; Disease Reservoirs; DNA, Intergenic; DNA, Protozoan; Genotype; Geography; Humans; Insect Vectors; Microsatellite Repeats; Molecular Sequence Data; Phylogeny; RNA, Spliced Leader; Sequence Alignment; Triatominae; Trypanosoma cruzi; Argentina; Chile; French Guiana; Panama [Central America]; United States; Venezuela; Didelphis virginiana; Mepraia; Mepraia spinolai; Rhodnius nasutus; Rhodnius neglectus; Triatoma gerstaeckeri; Triatoma infestans; Trypanosoma cruzi |
Año: | 2010
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Volumen: | 40
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Número: | 14
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Página de inicio: | 1599
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Página de fin: | 1607
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DOI: |
http://dx.doi.org/10.1016/j.ijpara.2010.06.006 |
Título revista: | International Journal for Parasitology
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Título revista abreviado: | Int. J. Parasitol.
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ISSN: | 00207519
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CODEN: | IJPYB
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CAS: | DNA, Intergenic; DNA, Protozoan; RNA, Spliced Leader
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v40_n14_p1599_Cura |
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Citas:
---------- APA ----------
Cura, C.I., Mejía-Jaramillo, A.M., Duffy, T., Burgos, J.M., Rodriguero, M., Cardinal, M.V., Kjos, S.,..., Schijman, A.G.
(2010)
. Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes. International Journal for Parasitology, 40(14), 1599-1607.
http://dx.doi.org/10.1016/j.ijpara.2010.06.006---------- CHICAGO ----------
Cura, C.I., Mejía-Jaramillo, A.M., Duffy, T., Burgos, J.M., Rodriguero, M., Cardinal, M.V., et al.
"Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes"
. International Journal for Parasitology 40, no. 14
(2010) : 1599-1607.
http://dx.doi.org/10.1016/j.ijpara.2010.06.006---------- MLA ----------
Cura, C.I., Mejía-Jaramillo, A.M., Duffy, T., Burgos, J.M., Rodriguero, M., Cardinal, M.V., et al.
"Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes"
. International Journal for Parasitology, vol. 40, no. 14, 2010, pp. 1599-1607.
http://dx.doi.org/10.1016/j.ijpara.2010.06.006---------- VANCOUVER ----------
Cura, C.I., Mejía-Jaramillo, A.M., Duffy, T., Burgos, J.M., Rodriguero, M., Cardinal, M.V., et al. Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes. Int. J. Parasitol. 2010;40(14):1599-1607.
http://dx.doi.org/10.1016/j.ijpara.2010.06.006