Abstract:
Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc.
Registro:
Documento: |
Artículo
|
Título: | Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
Autor: | Burgos, J.M.; Altcheh, J.; Bisio, M.; Duffy, T.; Valadares, H.M.S.; Seidenstein, M.E.; Piccinali, R.; Freitas, J.M.; Levin, M.J.; Macchi, L.; Macedo, A.M.; Freilij, H.; Schijman, A.G. |
Filiación: | Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Buenos Aires, Argentina Laboratorio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina Laboratório de Genética-Bioquímica, Departamento de Bioquímica e Imunologia, ICB, Belo Horizonte, Brazil Servicio de Neonatología y Obstetricia, Hospital Bernardino Rivadavia, Ciudad Autonoma de Buenos Aires, Argentina Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
|
Palabras clave: | Microsatellite loci; Phylogenetic lineage; Real time PCR; Transplacental transmission; Trypanosoma cruzi clonality; minicircle DNA; allele; blood; Chagas disease; disease transmission; genetic marker; genotype; molecular analysis; parasite transmission; phylogenetics; polymerase chain reaction; polymorphism; pregnancy; protozoan; adult; Argentina; article; blood sampling; Bolivia; Chagas disease; child; congenital infection; controlled study; endemic disease; female; gene amplification; gene locus; genetic analysis; genetic line; genotype; human; infant; major clinical study; newborn; parasite transmission; polymerase chain reaction; prevalence; restriction fragment length polymorphism; Trypanosoma; Trypanosoma cruzi; twins; vertical transmission; Adolescent; Adult; Animals; Argentina; Bolivia; Chagas Disease; Child; Child, Preschool; Disease Susceptibility; Disease Transmission, Vertical; DNA, Protozoan; Female; Humans; Infant; Infant, Newborn; Male; Mothers; Polymerase Chain Reaction; Pregnancy; Pregnancy Complications, Parasitic; Risk Factors; Trypanosoma cruzi; Argentina; Bolivia; South America; Trypanosoma cruzi |
Año: | 2007
|
Volumen: | 37
|
Número: | 12
|
Página de inicio: | 1319
|
Página de fin: | 1327
|
DOI: |
http://dx.doi.org/10.1016/j.ijpara.2007.04.015 |
Título revista: | International Journal for Parasitology
|
Título revista abreviado: | Int. J. Parasitol.
|
ISSN: | 00207519
|
CODEN: | IJPYB
|
CAS: | DNA, Protozoan
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v37_n12_p1319_Burgos |
Referencias:
- Barnabe, C., Neubauer, K., Solari, A., Tibayrenc, M., Trypanosoma cruzi: presence of the two major phylogenetic lineages and of several lesser discrete typing units (DTUs) in Chile and Paraguay (2001) Acta Trop., 78, pp. 127-137
- Brisse, S., Barnabe, C., Tibayrenc, M., Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electrophoresis (2000) Int. J. Parasitol., 30, pp. 34-44
- Brisse, S., Verhoef, J., Tibayrenc, M., Characterization of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages (2001) Int. J. Parasitol., 31, pp. 1218-1226
- Burgos, J.M., Begher, S., Freitas, J.M., Bisio, M., Duffy, T., Altcheh, J., Teijeiro, R., Schijman, A.G., Cerebral Chagas disease indicator of AIDS: Molecular diagnosis and typing of parasite populations involved in reactivation (2005) Am. J. Trop. Med. Hyg., 73, pp. 1016-1018
- Degrave, W., Fragoso, S.P., Britto, C., van Heuverswyn, H., Kidane, G.Z., Cardoso, M.A., Mueller, R.U., Morel, C.M., Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi (1988) Mol. Biochem. Parasitol., 27, pp. 63-70
- de Luca D'oro, G.M., Gardenal, C.N., Perret, B., Crisci, J.V., Montamat, E.E., Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism (1993) Parasitology, 107, pp. 405-410
- Diosque, P., Barnabe, C., Padilla, A.M., Marco, J.D., Cardozo, R.M., Cimino, R.O., Nasser, J.R., Basombrio, M.A., enzyme electrophoresis analysis of Trypanosoma cruzi isolates from a geographically restricted endemic area for Chagas' disease in Argentina (2003) Int. J. Parasitol., 33, pp. 997-1003
- Di Noia, J.M., Buscaglia, C.A., De Marchi, C.R., Almeida, I.C., Frasch, A.C.C., A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas' disease is due to a single parasite lineage (2002) J. Exp. Med., 195, pp. 401-413
- Fernandes, O., Sturm, N.R., Derré, R., Campbell, D.A., The mini-exon gene: a genetic marker for zymodeme III of Trypanosoma cruzi (1998) Mol. Biochem. Parasitol., 95, pp. 129-133
- Freilij, H., Altcheh, J., Congenital Chagas' disease: diagnostic and clinical aspects (1995) Clin. Infect. Dis., 21, pp. 551-555
- Freitas, J.M., Lages-Silva, E., Crema, E., Pena, S.D., Macedo, A.M., Real time PCR strategy for the identification of major lineages of Trypanosoma cruzi directly in chronically infected human tissues (2005) Int. J. Parasitol., 35, pp. 411-417
- Hermann, E., Truyens, C., Alonso-Vega, C., Rodriguez, P., Berthe, A., Torrico, F., Carlier, Y., Congenital transmission of Trypanosoma cruzi is associated with maternal enhanced parasitemia and decreased production of interferon-gamma in response to parasite antigens (2004) J. Infect. Dis., 189, pp. 1274-1281
- Jaccard, P., (1901) Bull. Soc. Vaudoisedes Sci. Nat., 37, pp. 241-272
- Kumar, S., Tamura, K., Nei, M., MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment (2004) Brief. Bioinform., 5, pp. 150-163
- Macedo, A.M., Pena, S.D.J., Genetic variability of Trypanosoma cruzi: implications for the pathogenesis of Chagas disease (1998) Parasitol. Today, 14, pp. 119-123
- Macedo, A.M., Pimenta, J.R., Aguiar, R.S., Melo, A.I., Chiari, E., Zingales, B., Pena, S.D., Oliveira, R.P., Usefulness of microsatellite typing in population genetic studies of T. cruzi (2001) Mem. Inst. Oswaldo Cruz, 96, pp. 407-413
- Marcet, P.L., Duffy, T., Cardinal, M.V., Burgos, J.M., Lauricella, M.A., Levin, M.J., Kitron, U., Schijman, A.G., PCR-based screening and lineage identification of Trypanosoma cruzi directly from faecal samples of triatomine bugs from northwestern Argentina (2006) Parasitology, 132, pp. 57-65
- Menezes, C.A., Bitterncourt, A.L., Mota, E., Sherlock, I., Ferreira, J., The assessment of parasitemia in women who are carriers of Trypanosoma cruzi infection during and after pregnancy (1992) Rev. Soc. Bras. Med. Trop., 25, pp. 109-113
- Oliveira, R.P., Broude, N.E., Macedo, A.M., Cantor, C.R., Smith, C.L., Pena, S.D., Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites (1998) Proc. Natl. Acad. Sci. USA, 9, pp. 3776-3780
- Saitou, N., Nei, M., The Neighbor-joining method: a new method for reconstructing phylogenetic trees (1987) Mol. Biol. Evol., 4, pp. 406-425
- Schijman, A.G., Altcheh, J., Burgos, J.M., Biancardi, M., Bisio, M., Levin, M.J., Freilij, H., Etiological treatment of congenital Chagas disease diagnosed and monitored by the polymerase chain reaction (2003) J. Antimicrob. Chemother., 52, pp. 441-449
- Schijman, A.G., Congenital Chagas disease (2006) Congenital and Other Related Infectious Diseases of the Newborn Perspectives in Medical Virology, 13, pp. 223-259. , Mushahwar I.K. (Ed), Elsevier B.V. Chapter 13
- Souto, R.P., Fernandes, O., Macedo, A.M., Campbell, D.A., Zingales, B., DNA markers define two major phylogenetic lineages of Trypanosoma cruzi (1996) Mol. Biochem. Parasitol., 83, pp. 141-152
- Virreira, M., Serrano, G., Maldonado, L., Svoboda, M., Trypanosoma cruzi: typing of genotype (sub)lineages in megacolon samples from bolivian patients (2006) Acta Trop., 100, pp. 252-255
- Virreira, M., Alonso-Vega, C., Solano, M., Jijena, J., Brutus, L., Bustamante, Z., Truyens, C., Svoboda, M., Congenital Chagas disease in Bolivia is not associated with DNA polymorphism of Trypanosoma cruzi (2006) Am. J. Trop. Med. Hyg., 75, pp. 871-879;
Citas:
---------- APA ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., Piccinali, R.,..., Schijman, A.G.
(2007)
. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. International Journal for Parasitology, 37(12), 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015---------- CHICAGO ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al.
"Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease"
. International Journal for Parasitology 37, no. 12
(2007) : 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015---------- MLA ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al.
"Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease"
. International Journal for Parasitology, vol. 37, no. 12, 2007, pp. 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015---------- VANCOUVER ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. Int. J. Parasitol. 2007;37(12):1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015