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Burgos, J.M.; Altcheh, J.; Bisio, M.; Duffy, T.; Valadares, H.M.S.; Seidenstein, M.E.; Piccinali, R.; Freitas, J.M.; Levin, M.J.; Macchi, L.; Macedo, A.M.; Freilij, H.; Schijman, A.G. "Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease" (2007) International Journal for Parasitology. 37(12):1319-1327
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Abstract:

Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc.

Registro:

Documento: Artículo
Título:Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
Autor:Burgos, J.M.; Altcheh, J.; Bisio, M.; Duffy, T.; Valadares, H.M.S.; Seidenstein, M.E.; Piccinali, R.; Freitas, J.M.; Levin, M.J.; Macchi, L.; Macedo, A.M.; Freilij, H.; Schijman, A.G.
Filiación:Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Buenos Aires, Argentina
Laboratorio de Parasitología y Enfermedad de Chagas, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina
Laboratório de Genética-Bioquímica, Departamento de Bioquímica e Imunologia, ICB, Belo Horizonte, Brazil
Servicio de Neonatología y Obstetricia, Hospital Bernardino Rivadavia, Ciudad Autonoma de Buenos Aires, Argentina
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:Microsatellite loci; Phylogenetic lineage; Real time PCR; Transplacental transmission; Trypanosoma cruzi clonality; minicircle DNA; allele; blood; Chagas disease; disease transmission; genetic marker; genotype; molecular analysis; parasite transmission; phylogenetics; polymerase chain reaction; polymorphism; pregnancy; protozoan; adult; Argentina; article; blood sampling; Bolivia; Chagas disease; child; congenital infection; controlled study; endemic disease; female; gene amplification; gene locus; genetic analysis; genetic line; genotype; human; infant; major clinical study; newborn; parasite transmission; polymerase chain reaction; prevalence; restriction fragment length polymorphism; Trypanosoma; Trypanosoma cruzi; twins; vertical transmission; Adolescent; Adult; Animals; Argentina; Bolivia; Chagas Disease; Child; Child, Preschool; Disease Susceptibility; Disease Transmission, Vertical; DNA, Protozoan; Female; Humans; Infant; Infant, Newborn; Male; Mothers; Polymerase Chain Reaction; Pregnancy; Pregnancy Complications, Parasitic; Risk Factors; Trypanosoma cruzi; Argentina; Bolivia; South America; Trypanosoma cruzi
Año:2007
Volumen:37
Número:12
Página de inicio:1319
Página de fin:1327
DOI: http://dx.doi.org/10.1016/j.ijpara.2007.04.015
Título revista:International Journal for Parasitology
Título revista abreviado:Int. J. Parasitol.
ISSN:00207519
CODEN:IJPYB
CAS:DNA, Protozoan
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v37_n12_p1319_Burgos

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Citas:

---------- APA ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., Piccinali, R.,..., Schijman, A.G. (2007) . Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. International Journal for Parasitology, 37(12), 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015
---------- CHICAGO ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al. "Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease" . International Journal for Parasitology 37, no. 12 (2007) : 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015
---------- MLA ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al. "Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease" . International Journal for Parasitology, vol. 37, no. 12, 2007, pp. 1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015
---------- VANCOUVER ----------
Burgos, J.M., Altcheh, J., Bisio, M., Duffy, T., Valadares, H.M.S., Seidenstein, M.E., et al. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease. Int. J. Parasitol. 2007;37(12):1319-1327.
http://dx.doi.org/10.1016/j.ijpara.2007.04.015