Artículo

Gómez, E.B.; Medina, G.; Ballesta, J.P.G.; Levin, M.J.; Téllez-Ión, M.T. "Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi" (2001) International Journal for Parasitology. 31(10):1032-1039
La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Trypanosoma cruzi ribosomes from epimastigote forms were purified as determined by electron microscopy and isoelectrofocusing was used to analyse this purified ribosome fraction. Silver stained gels revealed that acidic proteins are present in at least 10 different isoforms, in accord with previous cloning studies. To detect phosphorylation, in vitro phosphorylation assays using the recombinant protein TcP2β-mbp were carried out. The results showed that T. cruzi cytosolic fraction possesses protein kinase activity able to phosphorylate the recombinant protein. Purified ribosomes contain protein kinases that could also phosphorylate the recombinant protein TcP2β-mbp. Labelling parasites with [32Pi] in a phosphate free medium demonstrated that ribosome proteins, recognised with a specific mouse antiserum against recombinant TcP2β proteins, are phosphorylated in vivo. All these results suggest that in vivo phosphorylation of ribosome TcP2β proteins are mediated by protein kinase(s) not yet identified. © 2001 Australian Society for Parasitology Inc.

Registro:

Documento: Artículo
Título:Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi
Autor:Gómez, E.B.; Medina, G.; Ballesta, J.P.G.; Levin, M.J.; Téllez-Ión, M.T.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Canto Blanco, 28049, Madrid, Spain
Palabras clave:Isoelectrofocusing; P proteins; Protein phosphorylation; Ribosome; Trypanosoma; acid protein; casein kinase II; isoprotein; ribosome protein; article; controlled study; enzyme activity; isoelectric focusing; nonhuman; protein phosphorylation; regulatory mechanism; ribosome; Trypanosoma cruzi; Animals; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Microscopy, Electron; Phosphorylation; Protozoan Proteins; Recombinant Proteins; Ribosomal Proteins; Ribosomes; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi
Año:2001
Volumen:31
Número:10
Página de inicio:1032
Página de fin:1039
DOI: http://dx.doi.org/10.1016/S0020-7519(01)00219-3
Título revista:International Journal for Parasitology
Título revista abreviado:Int. J. Parasitol.
ISSN:00207519
CODEN:IJPYB
CAS:L12E protein, Trypanosoma cruzi; Protozoan Proteins; Recombinant Proteins; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v31_n10_p1032_Gomez

Referencias:

  • Ballesta, J.P.G., Remacha, M., The large ribosomal subunit stalk as a regulatory element of the eukaryotic translational machinery (1996) Prog. Nucleic Acid Res. Mol. Biol., 55, pp. 157-193
  • Ballesta, J.P.G., Remacha, M., Naranda, T., Santos, C., Bermejo, B., Jimenez-Diaz, A., Ortiz-Reyes, B.L., The acidic ribosomal proteins and the control of protein synthesis in yeast (1993) Protein Synthesis and Targeting in Yeast, , Brown, A.J.P., Tuite, M.F., McCarthy, J.E.G. (Eds.), Springer, Berlin
  • Beltrame, M., Bianchi, M.E., Sequence of cDNA for one acidic ribosomal protein of Schizosaccharomyces pombe (1990) Mol. Cell. Biol., 10, pp. 2341-2348
  • Bielka, H., (1982) The Eukaryotic Ribosome Springer-Verlag, , Berlin, Heidelberg, New York
  • Borda, E., Pascual, J., Cossio, P., De la Vega, M., Arana, R., Sterin-Borda, L., A circulating IgG in Chagas' disease which binds to β-adrenergic receptor of myocardium and modulates their activity (1984) Clin. Exp. Immunol., 5, pp. 679-686
  • Bravo, T., Ansorge, V., (1982) Proceeding of Electrophoresis, , de Gruyter, W. (Ed.). Stathatos, D, Berlin
  • Egebjerg, J., Douthwaite, S., Liljas, A., Garret, R.A., Characterisation of the binding sites of protein L11 and the L10 (L12) pentameric complex in the GTPase domain of 23S ribosomal RNA from Escherichia coli (1990) J. Mol. Biol., 213, pp. 275-288
  • Elkon, K.B., Skelly, S., Parnassa, A., Möller, W., Danho, W., Weissbach, H., Brot, N., Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 7419-7423
  • Gómez, M.L., Erijman, L., Arauzo, S., Tones, H.N., Téllez-Iñón, M.T., Protein kinase C in Trypanosoma cruzi epimastigote forms: Partial purification and characterization (1989) Mol. Biochem. Parasitol., 36, pp. 101-108
  • Hansen, T.S., Andreasen, P.H., Dreisig, H., Hojrup, P., Nielsen, H., Engberg, J., Kristiansen, K., Tetrahymena thermophila acidic ribosomal protein L37 contains an archaebacterial type of C-terminus (1991) Gene, 105, pp. 143-150
  • Hasler, P., Brot, N., Weisbach, H., Parnassa, A.P., Elkon, K.B., Ribosomal proteins P0, P1, P2 are phosphorylated by casein kinase II at their conserved carboxil termini (1991) J. Biol. Chem., 266, pp. 13815-13820
  • Kaplan, D., Ferrari, I., López-Bergami, P., Mahler, E., Levitus, G., Chiale, P., Hoebeke, H., Levin, M.J., Antibodies to ribosomal P proteins of Trypanosoma cruzi in Chagas disease possess functional autoreactivity with heart tissue and differ from anti-P autoantibodies in lupus (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 10301-10306
  • Lavergne, J.P., Conquet, F., Reboud, J.P., Reboud, A.M., Role of acidic phosphoproteins in the partial reconstitution of the active 60S ribosomal subunit (1987) FEBS Lett., 216, pp. 83-88
  • Levin, M.J., Mesri, E., Benarous, R., Levitus, G., Schijman, A., Levy-Yeyati, P., Chiale, P.A., Segura, E.L., Identification of major Trypanosoma cruzi antigenic determinants in chronic Chagas heart disease (1989) Am. J. Trop. Med. Hyg., 41, pp. 530-539
  • Levin, M.J., Vazquez, M., Kaplan, D., Schijman, A.G., The Trypanosoma cruzi ribosomal P protein family: Classification and antigenicity (1993) Parasitol. Today, 9, pp. 381-384
  • Levitus, G., Hontebeyrie-Joskowicz, M., Van Regenmortel, M.H.V., Levin, M.J., Humoral autoimmune response to ribosomal P proteins in chronic Chagas' heart disease (1991) Clin. Exp. Immunol., 85, pp. 413-417
  • Levitus, G., Levin, M.J., Autoinmunidad en la enfermedad de Chagas (1998) Rev. Argent. Med., 1, pp. 12-16
  • Liljas, A., Comparative biochemistry and biophysics of ribosomal proteins (1991) Int. Rev. Cytol., 124, pp. 103-136
  • Lin, A., Wittman-Leibold, B., McNelly, T., Wool, I.G., The primary structure of the acidic phosphoprotein from rat liver 60S ribosomal subunit (1982) J. Biol. Chem., 257, pp. 9189-9197
  • Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V., Levin, M.J., Major Trypanosoma cruzi determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope (1990) J. Clin. Microbiol., 28, pp. 1219-1224
  • Mitsui, K., Tsurugi, K., cDNA and deduced amino acid sequence of acidic ribosomal A2 from Saccharomyces cerevisiae (1988) Nucleic Acids Res., 16, p. 3575
  • Naranda, T., Remacha, M., Ballesta, J.P.G., The activity-controlling Phosphorylation site is not the same in the four acidic ribosomal proteins from Saccharomyces cerevisiae (1993) J. Biol. Chem., 268, pp. 2451-2457
  • Ogueta, S.B., Solari, A., Téllez-Iñón, M.T., Trypanosoma cruzi epimastigote forms possess a Ca2 + /calmodulin-dependent protein kinase (1994) FEBS Lett., 337, pp. 293-297
  • Qian, S., Zhang, J.Y., Kay, M.A., Jacobs-Lorena, M., Structural analysis of the Drosophila rPA1 gene, a member of the eukaryotic 'A' type ribosomal protein family (1987) Nucleic Acids Res., 15, pp. 987-1003
  • Remacha, M., Jimenez-Diaz, A., Bermejo, B., Rodriguez-Gabriel, M.A., Guarinos, E., Ballesta, J.P.G., Ribosomal acidic phosphoproteins P1 and P2 are not required for cell viability but regulate the pattern of protein expression in Saccharomyces cerevisiae (1995) Mol. Cell. Biol., 15, pp. 4754-4762
  • Remacha, M., Jimenez-Diaz, A., Santos, C., Briones, E., Zambrano, R., Rodriguez-Gabriel, M.A., Guarinos, E., Ballesta, J.P.G., Proteins P1, P2 and P0, components of the eukariotic ribosome stalk. New structural and functional aspects (1995) Biochem. Cell Biol., 73, pp. 959-968
  • Rich, B.E., Steitz, J.A., Human acidic ribosomal phosphoproteins P0, P1 and P2: Analysis of cDNA clones, in vitro synthesis, and assembly (1987) Mol. Cell. Biol., 7, pp. 4065-4074
  • Rodriguez-Gabriel, M.A., Remacha, M., Ballesta, J.P.G., Phosphorylation of ribosomal protein P0 is not essential for ribosome function but affects translation (1998) Biochemistry, 37, pp. 16620-16626
  • Saenz-Robles, M.T., Remacha, M., Vilella, M.D., Zinker, S., Ballesta, J.P.G., The acidic ribosomal proteins as regulators of the eukaryotic ribosomal activity (1990) Biochim. Biophys. Acta, 1050, pp. 51-55
  • Sanchez-Madrid, F., Ballesta, J.P.G., An acidic protein associated to ribosomes of Saccharomyces cerevisiae. Changes during cell cycle (1979) Biochem. Biophys. Res. Commun., 91, pp. 643-650
  • Sandermann, J., Kruger, A., Kristiansen, K., Characterization of acidic 60S ribosomal proteins in Tetrahymena pyriformis (1979) FEBS Lett., 107, pp. 343-347
  • Santos, C., Ballesta, J.P.G., The highly conserved protein P0 carboxyl end is essential for ribosome activity only in the absence of proteins P1 and P2 (1995) J. Biol. Chem., 270, pp. 20608-20614
  • Schijman, A.G., Dusetti, N., Vazquez, M.P., Lafon, S., Levy-Yeyati, P., Levin, M.J., Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi acidic ribosomal P protein (P-JL5) (1990) Nucleic Acid Res., 18, p. 3399
  • Schijman, A.G., Vazquez, M.P., Ben Dov, C., Ghio, S., Lorenzi, H., Levin, M.J., Cloning and sequence analysis of the TcP2β cDNA variants of Trypanosoma cruzi (1995) Biochim. Biophys. Acta, 1264, pp. 15-18
  • Shimmin, L.C., Ramirez, G., Matheson, A.T., Dennis, P.P., Sequence alignment and evolutionary comparison of the L10 equivalent and L12 equivalent ribosomal proteins from archaebacterial, eubacterial and eukaryotes (1989) J. Mol. Evol., 29, pp. 448-462
  • Stöffler, G.E., Stöffler-Meilicke, M., Immunoelectron microscopy on Escherichia coli ribosomas (1986) Structure, Function and Genetics of Ribosomes, pp. 28-46. , Hardesty, B., Kramer, G. (Eds.), Springer-Verlag, New York
  • Szyszka, R., Boguszewska, A., Grankowski, N., Ballesta, J.P.G., Differential phosphorylation of ribosomal acidic proteins from yeast cell by two endogenous protein kinases; casein kinase-2 and 60S kinase (1995) Acta Bioch. Pol., 42, pp. 357-362
  • Szyszka, R., Bou, G., Ballesta, J.P.G., RAP kinase, a new enzyme phosphorylating the acidic P proteins from Saccharomyces cerevisiae (1996) Biochem. Biophys. Acta, 1293, pp. 213-221
  • Tsurugi, K., Collatz, E., Todokoro, K., Ulbrich, N., Lightfoot, H.Y., Wool, I.G., Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60S ribosomal subunit proteins La, Lb, Lf, P1, P2, L13′, L14, L20 and L38 (1978) J. Biol. Chem., 253, pp. 946-955
  • Uchiumi, T., Wahba, A.J., Traut, R.R., Topography and stoichiometry of acidic proteins in large ribosomal subunits from Artemia salina as determined by crosslinking (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 5580-5584
  • Unterkircher, C., Avrameas, S., Ternyck, T., Autoantibodies in the sera of Trypanosoma cruzi-infected individuals with or without clinical Chagas' disease (1993) J. Clin. Lab. Anal., 7, pp. 60-69
  • Van Agthoven, A., Kriek, J., Amons, R., Möller, W., Isolation and characterization of the acidic phosphoproteins of 60S ribosomes from Artemia salina and rat liver (1978) Eur. J. Biochem., 91, pp. 553-556
  • Vard, C., Guillot, D., Bargis, P., Lavergne, J.P., Reboud, J.P., A specific role for the phosphorylation of mammalian acidic ribosomal protein P2 (1997) J. Biol. Chem., 272, pp. 20259-20262
  • Vidales, F.J., Sáenz-Robles, M.T., Ballesta, J.P.G., Acidic proteins of the large ribosomal subunit in Saccharomyces cerevisiae. Effect of phosphorylation (1984) Biochemistry, 23, pp. 390-396
  • Vilella, M.D., Remacha, M., Ortiz, B.L., Méndez, E., Ballesta, J.P.G., Characterization of the yeast acidic ribosomal phosphoproteins using monoclonal antibodies. Proteins L44/45 and L440′ have different functional roles (1991) Eur. J. Biochem., 196, pp. 407-414
  • Wigboldus, J.D., DNA and deduced amino acid sequence of Drosophila rp21C, another 'A' type ribosomal protein (1987) Nucleic Acids Res., 15, p. 10064
  • Wool, I.G., Chan, Y.-L., Glück, A., Structure and evolution of mammalian ribosomal proteins (1995) Biochem. Cell Biol., 73, pp. 933-947
  • Zambrano, R., Briones, E., Remacha, M., Ballesta, J.P.G., Phosphorylation of the acidic ribosomal P proteins in Saccharomyces cerevisiae: A reappraisal (1997) Biochemistry, 36, pp. 14439-14446

Citas:

---------- APA ----------
Gómez, E.B., Medina, G., Ballesta, J.P.G., Levin, M.J. & Téllez-Ión, M.T. (2001) . Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi. International Journal for Parasitology, 31(10), 1032-1039.
http://dx.doi.org/10.1016/S0020-7519(01)00219-3
---------- CHICAGO ----------
Gómez, E.B., Medina, G., Ballesta, J.P.G., Levin, M.J., Téllez-Ión, M.T. "Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi" . International Journal for Parasitology 31, no. 10 (2001) : 1032-1039.
http://dx.doi.org/10.1016/S0020-7519(01)00219-3
---------- MLA ----------
Gómez, E.B., Medina, G., Ballesta, J.P.G., Levin, M.J., Téllez-Ión, M.T. "Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi" . International Journal for Parasitology, vol. 31, no. 10, 2001, pp. 1032-1039.
http://dx.doi.org/10.1016/S0020-7519(01)00219-3
---------- VANCOUVER ----------
Gómez, E.B., Medina, G., Ballesta, J.P.G., Levin, M.J., Téllez-Ión, M.T. Acidic ribosomal P proteins are phosphorylated in Trypanosoma cruzi. Int. J. Parasitol. 2001;31(10):1032-1039.
http://dx.doi.org/10.1016/S0020-7519(01)00219-3