Artículo

Dong, Q.G.; Graziani, A.; Garlanda, C.; Wainstok De Calmanovici, R.; Arese, M.; Soldi, R.; Vecchi, A.; Mantovani, A.; Bussolino, F. "Anti-tumor activity of cytokines against opportunistic vascular tumors in mice" (1996) International Journal of Cancer. 65(5):700-708
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Abstract:

Polyoma middle T (PmT)-transformed endothelial cells may represent a unique murine model for human opportunistic vascular tumors. The present study was designed to evaluate the anti-tumor potential of a panel of 13 cytokines against murine PmT-transformed endothelial cells. Interferon gamma (IFNγ) and transforming growth factor beta 1 (TGFβ1) substantially decreased in a dose-dependent manner the proliferation of a panel of 6 PmT-transformed cell lines. IFNα and tumor necrosis factor α (TNFα) had marginal anti-proliferative activity, whereas other molecules (interleukins-1, -2, -4, -6 and -13, IFNβ, leukemia inhibitory factor, oncostatin M, granulocyte-macrophage colony-stimulating factor) caused no growth inhibition. IFNγ and TGFβ1 were therefore selected for further analysis of their mechanism of action and in vivo relevance. IFNγ and TGFβ1 reduced the activity of phosphatidylinositol-3-kinase and the production of phosphatidylinositol 3,4-biphosphate, without modifying the tyrosine kinase(s) activity associated with PmT. IFNγ and TGFβ1 were also tested for their ability to modify the in vivo growth of the PmT-transformed endothelial cells H5V in syngeneic C57B1/6 mice. Treatment with IFNα and TGFβ1 significantly delayed tumor growth and increased survival time. In contrast, treatment with IFNα and TNFα failed to prolong survival. In nude mice, IFNα and TGFβ1 1 had a transient effect on tumor growth but no effect on survival, suggesting a contribution of T cells to the in vivo anti-tumor activity of these cytokines.

Registro:

Documento: Artículo
Título:Anti-tumor activity of cytokines against opportunistic vascular tumors in mice
Autor:Dong, Q.G.; Graziani, A.; Garlanda, C.; Wainstok De Calmanovici, R.; Arese, M.; Soldi, R.; Vecchi, A.; Mantovani, A.; Bussolino, F.
Filiación:Ist. Ric. Farmacologiche Mario Negri, Milan, Italy
Dipto. di Sci. Biomediche e Oncol., Università di Torino, Turin, Italy
Departamento de Quimica Biologica, Universidad de Buenos Aires, Buenos Aires, Argentina
Dipto. Genet., Biol. e Chim. Medica, Università di Torino, Turin, Italy
Department of Immunology, Ist. Ric. Farmacologiche Mario Negri, Via Eritrea 62, 20157 Milan, Italy
Palabras clave:alpha interferon; beta interferon; gamma interferon; granulocyte macrophage colony stimulating factor; interleukin 1; interleukin 13; interleukin 2; interleukin 4; interleukin 6; leukemia inhibitory factor; oncostatin m; transforming growth factor beta; tumor necrosis factor alpha; animal cell; animal model; article; cell transformation; controlled study; endothelium cell; growth inhibition; mouse; priority journal; vascular tumor; 1-Phosphatidylinositol 3-Kinase; Animals; Antigens, Polyomavirus Transforming; Cell Division; Cell Transformation, Viral; Cytokines; Female; Hemangioendothelioma; Mice; Mice, Inbred C57BL; Mice, Nude; Phosphatidylinositols; Phosphorylation; Phosphotransferases (Alcohol Group Acceptor); Tumor Cells, Cultured; Vascular Neoplasms
Año:1996
Volumen:65
Número:5
Página de inicio:700
Página de fin:708
DOI: http://dx.doi.org/10.1002/(SICI)1097-0215(19960301)65:5<700::AID-IJC23>3.0.CO;2-9
Título revista:International Journal of Cancer
Título revista abreviado:INT. J. CANCER
ISSN:00207136
CODEN:IJCNA
CAS:1-Phosphatidylinositol 3-Kinase, EC 2.7.1.137; Antigens, Polyomavirus Transforming; Cytokines; Phosphatidylinositols; Phosphotransferases (Alcohol Group Acceptor), EC 2.7.1.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207136_v65_n5_p700_Dong

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Citas:

---------- APA ----------
Dong, Q.G., Graziani, A., Garlanda, C., Wainstok De Calmanovici, R., Arese, M., Soldi, R., Vecchi, A.,..., Bussolino, F. (1996) . Anti-tumor activity of cytokines against opportunistic vascular tumors in mice. International Journal of Cancer, 65(5), 700-708.
http://dx.doi.org/10.1002/(SICI)1097-0215(19960301)65:5<700::AID-IJC23>3.0.CO;2-9
---------- CHICAGO ----------
Dong, Q.G., Graziani, A., Garlanda, C., Wainstok De Calmanovici, R., Arese, M., Soldi, R., et al. "Anti-tumor activity of cytokines against opportunistic vascular tumors in mice" . International Journal of Cancer 65, no. 5 (1996) : 700-708.
http://dx.doi.org/10.1002/(SICI)1097-0215(19960301)65:5<700::AID-IJC23>3.0.CO;2-9
---------- MLA ----------
Dong, Q.G., Graziani, A., Garlanda, C., Wainstok De Calmanovici, R., Arese, M., Soldi, R., et al. "Anti-tumor activity of cytokines against opportunistic vascular tumors in mice" . International Journal of Cancer, vol. 65, no. 5, 1996, pp. 700-708.
http://dx.doi.org/10.1002/(SICI)1097-0215(19960301)65:5<700::AID-IJC23>3.0.CO;2-9
---------- VANCOUVER ----------
Dong, Q.G., Graziani, A., Garlanda, C., Wainstok De Calmanovici, R., Arese, M., Soldi, R., et al. Anti-tumor activity of cytokines against opportunistic vascular tumors in mice. INT. J. CANCER. 1996;65(5):700-708.
http://dx.doi.org/10.1002/(SICI)1097-0215(19960301)65:5<700::AID-IJC23>3.0.CO;2-9