Artículo

Giulianelli, S.; Cerliani, J.P.; Lamb, C.A.; Fabris, V.T.; Bottino, M.C.; Gorostiaga, M.A.; Novaro, V.; Góngora, A.; Baldi, A.; Molinolo, A.; Lanari, C. "Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis" (2008) International Journal of Cancer. 123(11):2518-2531
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Abstract:

The mechanisms by which mammary carcinomas acquire hormone independence are still unknown. To study the role of cancer-associated fibroblasts (CAF) in the acquisition of hormone-independence we used a hormone-dependent (HD) mouse mammary tumor and its hormone-independent (HI) variant, which grows in vivo without hormone supply. HI tumors express higher levels of FGFR-2 than HD tumors. In spite of their in vivo differences, both tumors have the same hormone requirement in primary cultures. We demonstrated that CAF from HI tumors (CAF-HI) growing in vitro, express higher levels of FGF-2 than HD counterparts (CAF-HD). FGF-2 activated the progesterone receptors (PR) in the tumor cells, thus increasing cell proliferation in both HI and HD tumors. CAF-HI induced a higher proliferative rate on the tumor cells and in PR activation than CAF-HD. The blockage of FGF-2 in the co-cultures or the genetic or pharmacological inhibition of FGFR-2 inhibited PR activation and tumor cell proliferation. Moreover, in vivo, the FGFR inhibitor decreased C4-HI tumor growth, whereas FGF-2 was able to stimulate C4-HD tumor growth as MPA. T47D human breast cancer cells were also stimulated by progestins, FGF-2 or CAF-HI, and this stimulation was abrogated by antiprogestins, suggesting that the murine C4-HI cells respond as the human T47D cells. In summary, this is the first study reporting differences between CAF from HD and HI tumors suggesting that CAF-HI actively participate in driving HI tumor growth. © 2008 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis
Autor:Giulianelli, S.; Cerliani, J.P.; Lamb, C.A.; Fabris, V.T.; Bottino, M.C.; Gorostiaga, M.A.; Novaro, V.; Góngora, A.; Baldi, A.; Molinolo, A.; Lanari, C.
Filiación:Laboratory of Hormonal Carcinogenesis, Institute of Experimental Biology and Medicine (IBYME), National Research Council of Argentina (CONICET), Buenos Aires, Argentina
Oral and Pharyngeal Cancer Branch, NIDCR, NIH, Bethesda, MD 20892-4340, United States
IBYME, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Carcinoma associated fibroblasts; FGF receptors; Fibroblast growth factor 2; Hormone dependence; Progesterone receptors and breast cancer; 1 tert butyl 3 [6 (3,5 dimethoxyphenyl) 2 (4 diethylaminobutylamino)pyrido[2,3 d]pyrimidin 7 yl]urea; antigestagen; estrogen receptor; fibroblast growth factor 2; fibroblast growth factor receptor 2; medroxyprogesterone acetate; mifepristone; progesterone receptor; animal cell; animal experiment; animal model; animal tissue; article; breast carcinoma; cancer associated fibroblast; cancer cell; cancer growth; cell culture; cell proliferation; cell viability; coculture; controlled study; disease association; DNA binding; epithelium cell; female; fibroblast; gel mobility shift assay; hormonal regulation; hormone dependence; hormone response; immunofluorescence; immunohistochemistry; mouse; nonhuman; priority journal; protein expression; Western blotting; Animals; Breast Neoplasms; Cell Proliferation; Cells, Cultured; Coculture Techniques; Female; Fibroblast Growth Factor 2; Fibroblasts; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Inbred BALB C; Progesterone; Protein Binding; Protein Kinase Inhibitors; Receptor, Fibroblast Growth Factor, Type 2; Receptors, Progesterone; RNA, Small Interfering; Signal Transduction
Año:2008
Volumen:123
Número:11
Página de inicio:2518
Página de fin:2531
DOI: http://dx.doi.org/10.1002/ijc.23802
Título revista:International Journal of Cancer
Título revista abreviado:Int. J. Cancer
ISSN:00207136
CODEN:IJCNA
CAS:medroxyprogesterone acetate, 71-58-9; mifepristone, 84371-65-3; Fibroblast Growth Factor 2, 103107-01-3; Progesterone, 57-83-0; Protein Kinase Inhibitors; RNA, Small Interfering; Receptor, Fibroblast Growth Factor, Type 2, 2.7.10.1; Receptors, Progesterone
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207136_v123_n11_p2518_Giulianelli

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Citas:

---------- APA ----------
Giulianelli, S., Cerliani, J.P., Lamb, C.A., Fabris, V.T., Bottino, M.C., Gorostiaga, M.A., Novaro, V.,..., Lanari, C. (2008) . Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. International Journal of Cancer, 123(11), 2518-2531.
http://dx.doi.org/10.1002/ijc.23802
---------- CHICAGO ----------
Giulianelli, S., Cerliani, J.P., Lamb, C.A., Fabris, V.T., Bottino, M.C., Gorostiaga, M.A., et al. "Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis" . International Journal of Cancer 123, no. 11 (2008) : 2518-2531.
http://dx.doi.org/10.1002/ijc.23802
---------- MLA ----------
Giulianelli, S., Cerliani, J.P., Lamb, C.A., Fabris, V.T., Bottino, M.C., Gorostiaga, M.A., et al. "Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis" . International Journal of Cancer, vol. 123, no. 11, 2008, pp. 2518-2531.
http://dx.doi.org/10.1002/ijc.23802
---------- VANCOUVER ----------
Giulianelli, S., Cerliani, J.P., Lamb, C.A., Fabris, V.T., Bottino, M.C., Gorostiaga, M.A., et al. Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis. Int. J. Cancer. 2008;123(11):2518-2531.
http://dx.doi.org/10.1002/ijc.23802