Abstract:
1. 1. Fully reversible and highly stable biospecific adsorbents have been prepared by attaching delta aminolaevulinate to cyanogen bromide activated-Sepharose 4B, either directly or through extension arms. 2. 2. Purification of bovine liver delta aminolaevulinate dehydratase by means of these selective adsorbents is reported. These materials adsorb the enzyme quantitatively from partially purified preparations, and elution of delta aminolaevulinate dehydratase is achieved by modifying the ionic strength of the buffer. © 1977.
Registro:
Documento: |
Artículo
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Título: | Porphyrin biosynthesis-Immobilized enzymes and ligands-V. Purification of aminolaevulinate dehydratase from bovine liver by affinity chromatography |
Autor: | Stella, A.M.; del C. Batlle, A.M. |
Filiación: | Laboratorio de Porfirinas Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Argentina
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Palabras clave: | liver enzyme; porphobilinogen synthase; porphyrin; cattle; in vitro study; methodology; theoretical study |
Año: | 1977
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Volumen: | 8
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Número: | 5
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Página de inicio: | 353
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Página de fin: | 358
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DOI: |
http://dx.doi.org/10.1016/0020-711X(77)90004-0 |
Título revista: | International Journal of Biochemistry
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Título revista abreviado: | Int. J. Biochem.
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ISSN: | 0020711X
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CODEN: | IJBOB
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CAS: | porphobilinogen synthase, 9036-37-7; porphyrin, 24869-67-8
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v8_n5_p353_Stella |
Referencias:
- Batlle A.M. del, Ferramola, Grinstein, Purification and general properties of delta aminolaevulic dehydratase from cow liver (1967) Biochem. J., 104, pp. 244-249
- Batlle A.M. del, Ferramola, Grinstein, Delta aminolaevulinate dehydratase (cow liver) (1970) Methods in Enzymology, 17, pp. 216-220. , Tabor & Tabor, Academic Press, New York
- Batlle A.M. del, Llambias, Wider de Xifra, Tigier, Porphyrin biosynthesis in the soybean callus tissue system-XV The effect of growth conditions (1975) International Journal of Biochemistry, 3, pp. 591-606
- Batlle A.M. Del, Tigier, Llambias, Wider de Xifra, Biosintesis de porfirinas an callos de soya-XIV. Cambios en la actividad de la delta amino-levulico dehidrasa con las condiciones de crecimiento (1976) Anal. Asoc. Quim. Argent., 63, pp. 1-8
- Cuatrecasas, Protein purification by affinity chromatography. Derivatizations of agarose and polyacryl-amide beads (1970) J. biol. Chem., 245, pp. 3059-3065
- Cuatrecasas, Selective adsorbents based on biochemical specificity (1971) Biochemical Aspects of Reactions Solid Supports, pp. 79-110. , G.R. Stark, Academic Press, New York
- Cuatrecasas, Anfinsen, Affinity chromatography (1971) Ann. Rev. Biochem., 40, pp. 259-278
- Cuatrecasas, Welchek, Anfinsen, Selective enzyme purification by affinity chromatography (1968) Proc. natn. Acad. Sci., U.S.A., 61, pp. 636-643
- Ferramola, Batlle A.M. del, Tigier, Sancovich, Studies on delta aminolaevulinate dehydratase. Number and reactivity of sulfhydryl groups (1972) Anal. Asoc. Quim. Argent., 60, pp. 227-237
- Ferramola, Stella, Batlle A.M. del, Grinstein, Further properties of cow liver Delta aminolaevulinate dehydratase (1976) Acta Phys. Lat., , (In preparation)
- Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275
- Mauzerall, Granick, The occurrence and determination of delta-aminolevulinic acid and porpho-bilinogen in urine (1956) J. biol. Chem., 219, pp. 435-445
- Nandi, Sehmin, δ-Aminolevulinic acid dehydratase of Rhodopseudomonas spheroides—III. Mechanism of porphobilinogen synthesis (1968) J. biol. Chem., 243, pp. 1236-1242
- Stella, Batlle A.M. del, Porphyrin biosynthesis. Immobilized enzymes and ligands- VI. Studies on delta aminolaevulinate dehydratase from Euglena gracilis (1976) Act. Phys. Lot., , (in press)
- Stella, Batlle A.M. del, (1976) Porphyrin biosynthesis in Euglena gracilis-III. Light mediated increase in activity of delta amino-laevulinate dehydratase, , (In preparation)
- Stella, Batlle A.M. del, (1976) Porphyrin biosynthesis in Euglena gracilis-IV. Purification and properties of delta aminolaevulinate dehydratase, , (In preparation)
- Stella, Batlle A.M. del, (1976) Porphyrin biosynthesis in Euglena gracilis- -V. Effect of additions on the activity of delta aminolaevulinate dehydratase, , (In preparation)
- Stella, Wider de Xifra, Batlle A.M. del, Porphyrin biosynthesis. Immobilized enzymes-IV. Studies on aminolaevulate dehydratase attached to Sepharose (1976) Mol, Cell. Biochem., , (in press)
- Tigier, Battle A.M. del, Locascio, Porphyrin biosynthesis in soybean callus tissue system. Isolation, purification and general properties of delta aminolaevulinate dehydratase (1968) Biochim. biophys. Acta, 151, pp. 300-302
- Tigier, Batlle A.M. del, Locascio, Porphyrin biosynthesis in soybean callus tissue-III. Improved purification and some properties of delta aminolaevulinate dehydratase (1970) Enzymology, 38, pp. 43-56
- Tomio, Tuzman, Grinstein, Delta aminolaevulinate dehydratase from rat Harderian gland. Purification and properties (1968) Eur. J. Biochem., 6, pp. 84-87
- Wider de Xifra, Stella, Batlle A.M. del, Porphyrin biosynthesis. Immobilized enzymes and ligands-VIII. Studies on delta aminolae-vulinate synthetase from cultured soya-bean cells (1977) Molt. Cell. Biochem., , (in press)
Citas:
---------- APA ----------
Stella, A.M. & del C. Batlle, A.M.
(1977)
. Porphyrin biosynthesis-Immobilized enzymes and ligands-V. Purification of aminolaevulinate dehydratase from bovine liver by affinity chromatography. International Journal of Biochemistry, 8(5), 353-358.
http://dx.doi.org/10.1016/0020-711X(77)90004-0---------- CHICAGO ----------
Stella, A.M., del C. Batlle, A.M.
"Porphyrin biosynthesis-Immobilized enzymes and ligands-V. Purification of aminolaevulinate dehydratase from bovine liver by affinity chromatography"
. International Journal of Biochemistry 8, no. 5
(1977) : 353-358.
http://dx.doi.org/10.1016/0020-711X(77)90004-0---------- MLA ----------
Stella, A.M., del C. Batlle, A.M.
"Porphyrin biosynthesis-Immobilized enzymes and ligands-V. Purification of aminolaevulinate dehydratase from bovine liver by affinity chromatography"
. International Journal of Biochemistry, vol. 8, no. 5, 1977, pp. 353-358.
http://dx.doi.org/10.1016/0020-711X(77)90004-0---------- VANCOUVER ----------
Stella, A.M., del C. Batlle, A.M. Porphyrin biosynthesis-Immobilized enzymes and ligands-V. Purification of aminolaevulinate dehydratase from bovine liver by affinity chromatography. Int. J. Biochem. 1977;8(5):353-358.
http://dx.doi.org/10.1016/0020-711X(77)90004-0