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Abstract:

1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974.

Registro:

Documento: Artículo
Título:Thiol groups of soybean callus succinyl CoA synthetase
Autor:De Xifra, E.A.W.; Del C. Batlle, A.M.
Filiación:Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:5; 5′-dithio-bis-(2-nitrobenzoic acid); enzyme inactivation; kinetics; molecular weight changes; soybean callus tissue; subunits; Succinyl-CoA synthetase; thiol groups
Año:1974
Volumen:5
Número:2
Página de inicio:129
Página de fin:135
DOI: http://dx.doi.org/10.1016/0020-711X(74)90143-8
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0020711X_v5_n2_p129_DeXifra.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v5_n2_p129_DeXifra

Referencias:

  • Batlle, Estimation of molecular weights of proteins by Bio-Gel P Gel filtration (1967) Journal of Chromatography A, 28, pp. 82-88
  • Batlle, Del, Benson, Rimington, Purification and properties of coproporphyrinogenase (1965) Biochem J, 97, pp. 731-740
  • Bridger, Evidence for two types of subunits in succinyl CoA synthetase (1971) Biochem. Biophys. Res. Commun., 42, pp. 948-952
  • Ellman, A colorimetric method for determining low concentrations ofmercaptans (1958) Archs. Biochem. Biophys., 74, pp. 443-448
  • Ellman, Tissue sulfhydryl groups (1959) Archives of Biochemistry and Biophysics, 83, pp. 70-78
  • Grinnell, Ntshimura, The inactivation and dissociation of E. coli succinyl CoA synthetase by sulfhydryl reagents (1970) Biochim. Biophys. Acta, 212, pp. 150-157
  • Grinnell, Stollar, Nishimura, Reversible disaggregation of E. coli succinyl CoA synthetase (1969) Biochim. Biophys. Acta, 185, pp. 471-474
  • Leitzmann, Wu, Boyer, Subunits, composition and related properties of succinyl CoA synthetase (1970) Biochemistry, 9, pp. 2338-2346
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Ramaley, Bridger, Moyer, Boyer, The preparation properties and reactions of succinyl CoA synthetase and its phosphorylated form (1967) J. Biol. Chem., 242, pp. 4287-4298
  • Wider, Tigier, Properties and regulatory effect on tetrapyrrole biosynthesis of succinyl CoA synthetase isolated from soybean callus system (1970) FEBS Letters, 9, pp. 30-32
  • Wider, Tigier, Porphyrin biosynthesis in soybean callus tissue. VIII. Isolation, purification and general properties of succinyl CoA synthetase (1971) Enzymologia, 41, pp. 217-231

Citas:

---------- APA ----------
De Xifra, E.A.W. & Del C. Batlle, A.M. (1974) . Thiol groups of soybean callus succinyl CoA synthetase. International Journal of Biochemistry, 5(2), 129-135.
http://dx.doi.org/10.1016/0020-711X(74)90143-8
---------- CHICAGO ----------
De Xifra, E.A.W., Del C. Batlle, A.M. "Thiol groups of soybean callus succinyl CoA synthetase" . International Journal of Biochemistry 5, no. 2 (1974) : 129-135.
http://dx.doi.org/10.1016/0020-711X(74)90143-8
---------- MLA ----------
De Xifra, E.A.W., Del C. Batlle, A.M. "Thiol groups of soybean callus succinyl CoA synthetase" . International Journal of Biochemistry, vol. 5, no. 2, 1974, pp. 129-135.
http://dx.doi.org/10.1016/0020-711X(74)90143-8
---------- VANCOUVER ----------
De Xifra, E.A.W., Del C. Batlle, A.M. Thiol groups of soybean callus succinyl CoA synthetase. Int. J. Biochem. 1974;5(2):129-135.
http://dx.doi.org/10.1016/0020-711X(74)90143-8