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Abstract:

1. 1. The role of histidine on the decarboxylation of porphyrinogens of 7-, 6-, and 5-COOH III brought about by porphyrinogen carboxy-lyase (PCL) was studied. 2. 2. For this purpose hepatic PCL from normal and hexachlorobenzene (HCB) treated rats were modified with diethylpyrocarbonate. 3. 3. The results indicated that the enzyme from both normal and porphyric animals had histidine at the binding sites of all the porphyrinogens assayed. 4. 4. Comparative studies between the enzyme from normal and porphyric rats suggested that in vivo HCB treatment affected the active site for the decarboxylation of 7-, 6- and 5-COOH porphyrinogens III at histidine residues. 5. 5. On the other hand arginine modification by 2,3-butanedione treatment altered 5-COOH porphyrinogen III decarboxylation for both enzymes. However this amino acid was not involved at the binding site of this substrate. © 1994.

Registro:

Documento: Artículo
Título:Studies on the active centre(s) of rat liver porphyrinogen carboxy-lyase. in vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens
Autor:de Catabbi, S.C.B.; de Viale, L.C.S.M.
Filiación:Laboratorio de Porfirias Experimentales y Metabolismo del Hemo, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Nuñez 1428, Buenos Aires, Argentina [Fax 54-1-782-0620]
Palabras clave:hexachlorobenzene; porphyrinogen; uroporphyrinogen decarboxylase; animal experiment; animal model; article; binding site; carboxy terminal sequence; decarboxylation; drug effect; enzyme active site; enzyme binding; enzyme modification; female; nonhuman; porphyria; rat; Animal; Arginine; Binding Sites; Carboxy-Lyases; Decarboxylation; Diacetyl; Female; Hexachlorobenzene; Histidine; Liver; Porphyria; Porphyrinogens; Rats; Rats, Wistar; Support, Non-U.S. Gov't; Animalia
Año:1994
Volumen:26
Número:4
Página de inicio:595
Página de fin:600
DOI: http://dx.doi.org/10.1016/0020-711X(94)90019-1
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:Arginine, 74-79-3; Carboxy-Lyases, EC 4.1.1.; Diacetyl, 431-03-8; Hexachlorobenzene, 118-74-1; Histidine, 71-00-1; porphyrinogen carboxy-lyase, EC 4.1.1.-; Porphyrinogens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v26_n4_p595_deCatabbi

Referencias:

  • Batlle A.M. del, Grinstein, Porphyrin biosynthesis Phyriaporphyrinogen III a normal inter-mediate in the biosynthesis ofprotoporphyrin (1962) Biochimica et Biophysica Acta, 62, pp. 197-200
  • Billi de Catabbi, Rios de Molina, San Martin de Viale, Studies on the active centre of rat liver porphyrinogen carboxy-lyase. In vivo effect of hexachlorobencene (1991) Int. J. Biochem., 23, pp. 675-679
  • Burstein, Walsh, Neurah, Evidence of an essential histidine residue in termolysin (1974) Biochemistry, 13, pp. 205-210
  • Cantoni, Dal Fiume, Ruggieri, Decarboxylation of uroporphyrinogen I and III in 2,3,7,8-tetraehlorodibenzo-p-dioxin induced porphyria in mice (1984) Int. J. Biochem., 16, pp. 561-565
  • De Verneuil, Grandchamp, Norman, Some kinetic properties of human red cell uroporphyrinogen decarboxylase (1980) Biochim. biophys. Acta, 611, pp. 174-186
  • Elder, Evans, Matlin, The effect of porphyrinogenic compound, hexachlorobenzene, on the activity of hepatic uroporphyrinogen decarboxylase in the rat (1976) din. Sci. Molec. Med., 51, pp. 71-80
  • Elder, Tovey, Sheppard, Purification of uroporphyrinogen decarboxylase from human erythrocytes. Immunochemical evidence for a single protein with deearboxylase activity in human erythrocytes and liver (1983) Biochem. J., 215, pp. 46-55
  • Koopman, Juknat de Geralnik, Batlle, Porphyrin biosynthesis in Rhodopseudomonas palustris3-V. Purification of porphyrinogen decarboxylase and some unusual properties (1986) Int. J. Biochem., 18, pp. 935-944
  • Mauzerall, Granick, Porphyrin biosynthesis in erythrocytes III. Uroporphyrinogen and its decarboxylase (1958) J. biol. Chem., 232, pp. 1141-1162
  • Miles, Modification of histidyl residues in proteins by diethylpyrocarbonate (1977) Enzyme Structure, 47, pp. 431-442. , C.H.W. Hirs, S.N. Timasheff, Methods in Enzymology, Academic Press, New York, Part E
  • Rios de Molina, Wainstok de Calmanovici, San Martin de Viale, Liver porphyrinogen earboxylyase in hexachlorobenzene porphyric rats. Studies with intermediate porphyrinogens of series III and with uroporphyrinogen I (1987) Int. J. Biochem., 19, pp. 365-372
  • Rosen, Fedorcsak, Studies on the action of diethylpyrocarbonate on proteins (1966) Biochim. biophys. Acta, 130, pp. 404-405
  • San Martin de Viale, Aragones, Tomio, Tetrapyrroles as substrates and inhibitors of porphyrinogen earboxylyase from rat liver (1976) Acta physiol. latinoam., 34, pp. 393-400
  • San Martin de Viale, Grinstein, Porphyrin biosynthesis IV 5 and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis (1968) Biochimica et Biophysica Acta (BBA) - General Subjects, 158, pp. 79-91
  • San Martin de Viale, Rios de Molina, Wainstok de Calmanovicci, Tomio, Porphyrins and porphyrinogen carboxylyase in hexachlorobenzene induced porphyria (1977) Biochem. J., 168, pp. 393-400
  • San Martin de Viale, Viale, Nacht, Grinstein, Experimental porphyria induced in rats by hexachlorobenzene. A study of the porphyrins excreted by urine (1970) Clin. chim. Acta, 28, pp. 13-23
  • Straka, Kushner, Purification, characterization of bovine hepatic uroporphyrinogen decarboxylase (1983) Biochemistry, 22, pp. 4664-4672
  • Tomio, Garcia, San Martin de Viale, Grinstein, Porphyrin biosynthesis VII. Por phyrinogen carboxy-lyase from avian erythrocytes. Purification and properties (1970) Biochim. biophys. Acta, 198, pp. 353-363
  • Wainstok de Calmanovici, Rios de Molina, Taira de Yamasato, Tomio, San Martin de Viale, Mechanism of hexachlorobenzene induced porphyria in rats. Effect of phenobarbitone pretreatment (1984) Biochem. J., 218, pp. 753-763

Citas:

---------- APA ----------
de Catabbi, S.C.B. & de Viale, L.C.S.M. (1994) . Studies on the active centre(s) of rat liver porphyrinogen carboxy-lyase. in vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens. International Journal of Biochemistry, 26(4), 595-600.
http://dx.doi.org/10.1016/0020-711X(94)90019-1
---------- CHICAGO ----------
de Catabbi, S.C.B., de Viale, L.C.S.M. "Studies on the active centre(s) of rat liver porphyrinogen carboxy-lyase. in vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens" . International Journal of Biochemistry 26, no. 4 (1994) : 595-600.
http://dx.doi.org/10.1016/0020-711X(94)90019-1
---------- MLA ----------
de Catabbi, S.C.B., de Viale, L.C.S.M. "Studies on the active centre(s) of rat liver porphyrinogen carboxy-lyase. in vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens" . International Journal of Biochemistry, vol. 26, no. 4, 1994, pp. 595-600.
http://dx.doi.org/10.1016/0020-711X(94)90019-1
---------- VANCOUVER ----------
de Catabbi, S.C.B., de Viale, L.C.S.M. Studies on the active centre(s) of rat liver porphyrinogen carboxy-lyase. in vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens. Int. J. Biochem. 1994;26(4):595-600.
http://dx.doi.org/10.1016/0020-711X(94)90019-1