Abstract:
1. 1. This work represents the first approach to characterize the transport system of haem pathway precursors, such as δ-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. 2. ALA transport occurs unidirectionally by a sole active system with an apparent KM of 0.10 mM, at the optimum pH of 5.0. ALA uptake is influenced by both the carbon and nitrogen source; this suggests a rather complex regulation mechanism. 3. 3. This transport is not mediated by the general amino acid permease (GAP). 4. 4. ALA uptake is strongly inhibited by compounds harboring a methyl-amine terminus suggesting that this group is essential for ALA transport; however, the electric environment of the carboxylic group may be also important for the interaction between ALA and its transporter active site. 5. 5. We have found differences in ALA transport which would indicate a different regulation mechanism for this system in both strain cells. © 1993.
Registro:
Documento: |
Artículo
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Título: | δ-Aminolevulinic acid transport in Saccharomyces cerevisiae |
Autor: | Moretti, M.B.; García, S.C.; Stella, C.; Ramos, E.; Batlle, A.M.d.C. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Pabellon II, 2o Piso, 1428 Buenos Aires, Argentina Departamento de Bioquímica (CONICET, Facultad de Medicina, UBA), Paraguay 2155, 5o Piso, 1121 Buenos Aires, Argentina
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Palabras clave: | Saccharomyces cerevisiae; Saccharomyces cerevisiae; aminolevulinic acid; article; nonhuman; Saccharomyces cerevisiae; transport kinetics |
Año: | 1993
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Volumen: | 25
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Número: | 12
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Página de inicio: | 1917
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Página de fin: | 1924
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DOI: |
http://dx.doi.org/10.1016/0020-711X(88)90325-4 |
Título revista: | International Journal of Biochemistry
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Título revista abreviado: | Int. J. Biochem.
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ISSN: | 0020711X
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CODEN: | IJBOB
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CAS: | aminolevulinic acid, 106-60-5
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v25_n12_p1917_Moretti |
Referencias:
- Borralho, Panek, Malamud, Sanders, Mattoon, In situ assay for δ-aminolevulinate dehydratase and application to study of a catabolite repression-resistant Saccharomyces cerevisiae mutant (1983) J. Bact., 156, pp. 141-147
- Bray, A simple efficient aqueous solution in a liquid scintillation counter (1960) A. Biochem., 1, pp. 279-285
- Cheeks, Wedeen, Renal tubular transport of δ-aminolevulinic acid in rat (1986) Proc. Soc. exp. Biol. Med., 181, pp. 596-601
- Cooper, Metabolism and gene expression (1982) The Molecular Biology of the Yeast Saccharomyces cerevisiae, pp. 399-461. , J.N. Strathern, E.W. Jones, J.R. Broach, Cold Spring Harbor, New York
- Correa, Bermúdez Moretti, Cardalda, Rossetti, Batlle A.M. del, The role of ALA-S and ALA-D in regulating porphyrin biosynthesis in a normal and a HEM R+ mutant strain of Saccharomyces cerevisiae (1993) Yeast, 9, pp. 165-173
- Grenson, Crabeel, Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV Evidence for a general amino acid permease (1970) J. Bact., 103, pp. 770-777
- Herrada, Puppo, Rigaud, (δ-aminolevulinate uptake by Rhizobium bacteroids and its limitation by the peribacteroid membrane in legume nodules (1992) Biochem. biophys. Res. Commun., 184, pp. 1324-1330
- Jayaraman, Padmanaban, Malathi, Sarman, Haem synthesis during mitochondriogenesis in yeast (1972) Biochem. J., 121, pp. 531-535
- Kappas, Sassa, Anderson, The porphyrias (1983) Metabolic Bases of Inherited Diseases, pp. 1301-1384. , J. Sambury, J. Wyngaarden, D. Fredickson, J. Goldstein, M. Brown, 5th edition, McGraw-Hill, New York, Chapter 60
- Labbe-Bois, Volland, Changes in the activities of the protoheme-synthesizing system during the growth of yeast under different conditions (1977) Archives of Biochemistry and Biophysics, 179, pp. 565-577
- Labbe-Bois, Brouillet, Camadro, Chambon, Felix, Labbe, Rytka, Zagorec, Molecular approaches to heme biosynthesis in the yeast Saccharomyces cerevisiae (1986) Porphyrins and Porphyrias, 134, pp. 15-24. , Y. Nordmann, Colloque INSERM/John Libbey Eurotext Ltd
- Lasko, Brandriss, Proline transport in Saccharomyces cerevisiae (1981) J. Bact., 148, pp. 241-247
- Mattoon, Malamud, Brunner, Braz, Carvajal, Lancashire, Panek, Regulation of heme formation and cytochrome biosynthesis in normal and mutant yeast (1978) Biochemistry and Genetics of Yeast. Pured and Applied Aspects, pp. 317-337. , M. Bacilla, B. Horecker, A.O.M. Stoppani, Academic Press, New York
- Mitta, Ferramola, Sancovich, Grinstein, Improvement of the method of synthesis of δ-aminolevulinic acid-4-14C hydrochloride (1970) Journal of Labelled Compounds, 3, pp. 20-23
- Perlman, Mahler, Derepression of mitochondria and their enzymes in yeast: regulatory aspects (1974) Archs Biochem. Biophys., 162, pp. 248-271
- Polakis, Bartley, Changes in enzyme activities in Sacchromyces cerevisiae during aerobic growth on different carbon sources (1965) Biochem J, 97, pp. 284-297
- Ramos, Bongioanni, Stoppani, Kinetics of l-[14C]leucine transport in Saccharomyces cerevisiae (1980) Biochim. biophys. Acta, 599, pp. 214-231
- Roon, Larimore, Levy, Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae (1975) J. Bact., 124, pp. 325-331
- Roon, Levy, Larimore, Negative interactions between amino acid and methylamine/ ammonia transport systems of Saccharomyces cerevisiae (1977) J. biol. Chem., 252, pp. 3599-3604
- Vavra, Johnson, Aerobic and anaerobic biosynthesis of amino acids by baker's yeast (1956) J. biol. Chem., 220, pp. 33-43
- Woods, Sanders, Briquet, Foury, Drysdale, Mattoon, Regulation of mitochondrial biogenesis. Enzymatic changes in cytochrome-deficient yeast mutants requiring (δ-aminolevulinic acid (1975) J. biol. Chem., 250, pp. 9090-9098
Citas:
---------- APA ----------
Moretti, M.B., García, S.C., Stella, C., Ramos, E. & Batlle, A.M.d.C.
(1993)
. δ-Aminolevulinic acid transport in Saccharomyces cerevisiae. International Journal of Biochemistry, 25(12), 1917-1924.
http://dx.doi.org/10.1016/0020-711X(88)90325-4---------- CHICAGO ----------
Moretti, M.B., García, S.C., Stella, C., Ramos, E., Batlle, A.M.d.C.
"δ-Aminolevulinic acid transport in Saccharomyces cerevisiae"
. International Journal of Biochemistry 25, no. 12
(1993) : 1917-1924.
http://dx.doi.org/10.1016/0020-711X(88)90325-4---------- MLA ----------
Moretti, M.B., García, S.C., Stella, C., Ramos, E., Batlle, A.M.d.C.
"δ-Aminolevulinic acid transport in Saccharomyces cerevisiae"
. International Journal of Biochemistry, vol. 25, no. 12, 1993, pp. 1917-1924.
http://dx.doi.org/10.1016/0020-711X(88)90325-4---------- VANCOUVER ----------
Moretti, M.B., García, S.C., Stella, C., Ramos, E., Batlle, A.M.d.C. δ-Aminolevulinic acid transport in Saccharomyces cerevisiae. Int. J. Biochem. 1993;25(12):1917-1924.
http://dx.doi.org/10.1016/0020-711X(88)90325-4