Artículo
Abstract:
1. 1. The kinetics of porphyrin accumulation in cultured mammalian epithelial cells (CNCM-I-221) during exposure to ALA was investigated. 2. 2. The total porphyrin synthesized is a function of ALA concentration and the incubation time. The cellular porphyrin content exhibited a saturation pattern, reaching a plateau at about 0.04 fmol porphyrins/cell. A biphasic time-dependent increase in the total porphyrin synthesized was observed. 3. 3. After 3 hr of exposure to ALA the rate of synthesis increased to ahnost twice the initial rate, reaching between 0.02 and 0.05 fmol porphyrins/cell/hr depending on serum concentration in the medium. 4. 4. Two effects of FBS on ALA-stimulated porphyrin accumulation were observed. Greater total porphyrin synthesis was found when incubations were made in 10% FBS compared to those in 1% FBS. 5. 5. The higher serum concentration also caused a greater release into the medium of the porphyrins generated in the cells with a calculated half-life of 24 min in 10% serum-supplemented medium compared with 62 min in 1% serum. 6. 6. The results obtained from cell synchronization experiments suggest that there is little obvious cell cycle-dependent variation in the synthesis of porphyrins from ALA. 7. 7. The small differences in the intracellular porphyrin content that were observed may be attributed to a slight reduction in the rate of loss of porphyrins in G2/M cells. © 1993.
Registro:
| Documento: | Artículo |
| Título: | Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA |
| Autor: | Fukuda, H.; Batlle, A.M.C.; Riley, P.A. |
| Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Consejo Nacional de Investigaciones Cientificas Tecnicas (CONICET), Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina Division of Molecular Pathology, UCL Medical School, Windeyer Building, 46 Cleveland Street, London W1P6DB U.K. [Tel. 071-636-8333; Fax 071-387-3310], United Kingdom |
| Palabras clave: | aminolevulinic acid; porphyrin; animal cell; article; cell culture; epithelium cell; nonhuman; Aminolevulinic Acid; Animals; Cell Cycle; Cell Line; Epithelial Cells; Epithelium; Kinetics; Porphyrins; Animalia; Mammalia |
| Año: | 1993 |
| Volumen: | 25 |
| Número: | 10 |
| Página de inicio: | 1407 |
| Página de fin: | 1410 |
| DOI: | http://dx.doi.org/10.1016/0020-711X(93)90689-C |
| Título revista: | International Journal of Biochemistry |
| Título revista abreviado: | Int. J. Biochem. |
| ISSN: | 0020711X |
| CODEN: | IJBOB |
| CAS: | aminolevulinic acid, 106-60-5; porphyrin, 24869-67-8; Aminolevulinic Acid, 106-60-5; Porphyrins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v25_n10_p1407_Fukuda |
Referencias:
- Bedwell, MacRobert, Phillips, Bown, Fluorescence distribution and photodynamic effect of ALA-induced protoporphyrin IX in the DMH rate colonic tumour model (1992) Br.J. Cancer, 65, pp. 818-824
- Divaris, Kennedy, Pottier, Phototoxic damage to sebaceous glands and hair follicle of mice after systemic administration of 5-aminolevulic acid correlates with localized protoporphyrin IX fluorescence (1990) Am.J. Path., 136, pp. 891-897
- Dougherty, Photosensitizers, therapy and detection of malignant tumors (1987) Photochem. Photobiol., 45, pp. 879-889
- Fukuda, Paredes, Batlle, Tumor-localizing properties of porphyrins. In vitro studies using the porphyrin precursor aminolevulinic acid, in free and liposome encapsulated forms (1989) Drug Des. Deliv., 5, pp. 133-139
- Fukuda, Paredes, Batlle, Tumor-localizing properties of porphyrins. In vivo studies using free and liposome encapsulated aminolevulinic acid (1992) Comp. Biochem. Physiol., 102 B, pp. 433-436
- Fukuda, Paredes, Casas, Chueke, Batlle, Potential of liposome-entrapped aminolevulinic acid in cancer therapy. Effect of prior injection of empty liposomes and different routes of administration (1992) Cancer J., 5, pp. 295-299
- Granick, Sinclair, Sassa, Grieninger, Effects by heme, insulin, and serum albumin on heme and protein synthesis in chick embryo liver cells cultured in a chemically denned medium, and a spectrofluorometric assay for porphyrin composition (1975) J. biol. Chem., 250, pp. 9215-9225
- Kennedy, Pottier, Pross, Photodynamic therapy with endogenous protoporphyrin IX: basic principles and present clinical experience (1990) J. Photochem. Photobiol. (B), 6, pp. 143-148
- Kessel, Porphyrin localization: a new modality for detection and therapy of tumors (1984) Biochem. Pharmac., 33, pp. 1389-1393
- Malik, Lugaci, Destruction of erythroleukemic cells by photoactivation of endogenous porphyrins (1987) British Journal of Cancer, 156, pp. 589-595
- Moan, Berg, Photochemotherapy of cancer: experimental research-Yearly Review (1992) Photochem. Photobiol., 55, pp. 931-938
- Rebeiz, Rebeiz, Arkins, Kelley, Rebeiz, Photodestruction of tumor cells by induction of endogenous accumulation of protoporphyrin IX: enhancement by 1,10-phenanthroline (1992) Photochem. Photobiol., 55, pp. 431-435
- Riley, Dean, Phagocytosis of latex particles in relation to the cell cycle in 3T3 cells (1978) Exp. cell. Biol., 46, pp. 367-373
Citas:
---------- APA ----------
Fukuda, H., Batlle, A.M.C. & Riley, P.A. (1993) . Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA. International Journal of Biochemistry, 25(10), 1407-1410.http://dx.doi.org/10.1016/0020-711X(93)90689-C
---------- CHICAGO ----------
Fukuda, H., Batlle, A.M.C., Riley, P.A. "Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA" . International Journal of Biochemistry 25, no. 10 (1993) : 1407-1410.http://dx.doi.org/10.1016/0020-711X(93)90689-C
---------- MLA ----------
Fukuda, H., Batlle, A.M.C., Riley, P.A. "Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA" . International Journal of Biochemistry, vol. 25, no. 10, 1993, pp. 1407-1410.http://dx.doi.org/10.1016/0020-711X(93)90689-C
---------- VANCOUVER ----------
Fukuda, H., Batlle, A.M.C., Riley, P.A. Kinetics of porphyrin accumulation in cultured epithelial cells exposed to ALA. Int. J. Biochem. 1993;25(10):1407-1410.http://dx.doi.org/10.1016/0020-711X(93)90689-C
