Abstract:
1. 1. δ-Aminolevulinate-dehydratase (ALA-D) from Rhizobium japonicum and Rhizobium meliloti was isolated and some properties were studied. 2. 2. The enzyme from both strains require DTT to maintain full activity and a concentration of about 8 mM is necessary for its maximum expression. Thiol inactivating compounds and heavy metals ions such as Pb2+ and Cd2+ inactivate ALA-D. 3. 3. The enzyme exhibits Michaelis-Menten kinetics and has an apparent Km of 0.095 and 0.1-0.37 mM for Rhizobium japonicum and Rhizobium meliloti respectively. 4. 4. For both strains the pH profiles show a well denned maximum at about 7.2-7.6 and a second broad peak or shoulder in the range of pH 9-10. 5. 5. ALA-D from Rhizobium does not appear to be a heat stable enzyme as it happens to be in other sources. © 1992.
Registro:
Documento: |
Artículo
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Título: | Delta-aminolevulinate dehydratase from free-living Rhizobium |
Autor: | De Bonis, A.F.; Rossetti, M.V.; Del C. Batlle, A.M. |
Filiación: | Centre de Investigaciones sobre Porfirinas y Porfirias-CIPYP, (CONICET, FCE y N, UBA), Ciudad Universitaria, Pabellon II, 2 do Piso, 1428 Buenos Aires, Argentina
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Palabras clave: | porphobilinogen synthase; article; enzyme kinetics; nonhuman; ph; rhizobium; Bradyrhizobium japonicum; Rhizobium; Sinorhizobium meliloti |
Año: | 1992
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Volumen: | 24
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Número: | 11
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Página de inicio: | 1841
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Página de fin: | 1847
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DOI: |
http://dx.doi.org/10.1016/0020-711X(92)90136-O |
Título revista: | International Journal of Biochemistry
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Título revista abreviado: | Int. J. Biochem.
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ISSN: | 0020711X
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CODEN: | IJBOB
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v24_n11_p1841_DeBonis |
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Citas:
---------- APA ----------
De Bonis, A.F., Rossetti, M.V. & Del C. Batlle, A.M.
(1992)
. Delta-aminolevulinate dehydratase from free-living Rhizobium. International Journal of Biochemistry, 24(11), 1841-1847.
http://dx.doi.org/10.1016/0020-711X(92)90136-O---------- CHICAGO ----------
De Bonis, A.F., Rossetti, M.V., Del C. Batlle, A.M.
"Delta-aminolevulinate dehydratase from free-living Rhizobium"
. International Journal of Biochemistry 24, no. 11
(1992) : 1841-1847.
http://dx.doi.org/10.1016/0020-711X(92)90136-O---------- MLA ----------
De Bonis, A.F., Rossetti, M.V., Del C. Batlle, A.M.
"Delta-aminolevulinate dehydratase from free-living Rhizobium"
. International Journal of Biochemistry, vol. 24, no. 11, 1992, pp. 1841-1847.
http://dx.doi.org/10.1016/0020-711X(92)90136-O---------- VANCOUVER ----------
De Bonis, A.F., Rossetti, M.V., Del C. Batlle, A.M. Delta-aminolevulinate dehydratase from free-living Rhizobium. Int. J. Biochem. 1992;24(11):1841-1847.
http://dx.doi.org/10.1016/0020-711X(92)90136-O