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Abstract:

1. 1. Porphyrinogen carboxylyase from the liver of normal and hexachlorobenzene porphyric rats was subjeced to chemical modification using photo-oxidation with methylene blue, diethylpyrocarbonate, butane-2,3-dione, and phenylglyoxal. 2. 2. All of these chemicals inactivated the enzyme from both sources. 3. 3. Reversion of the diethylpyrocarbonate reaction with hydroxylamine as well as protection of the enzymes with uroporphyrinogen III indicated that histicline is involved at least in the first decarboxylation active site of the porphyrinogen carboxylyase, and perhaps in one or more sites where the removal of the other carboxyl groups take place. 4. 4. Arginine seems not to be at the active site of the enzyme but at its environment since two diketones alter the enzyme activity, however the substrate did not protect the enzyme from the butane-2,3-dione modification. 5. 5. Comparative studies between the enzyme from normal and porphyric animals suggest that the low enzyme activity from intoxicated animals could be due to alterations of its active centre environment produced by hexachlorobenzene treatment. This treatment seems to partially protect the active site of the porphyrinogen carboxylyase from the modification reactions. © 1991.

Registro:

Documento: Artículo
Título:Studies on the active centre of rat liver porphyrinogen carboxylyase in vivo effect of hexachlorobenzene
Autor:De Catabbi, S.B.; De Molina, M.D.C.R.; De Viale, L.C.S.M.
Filiación:Laboratorio de Porfirias Experimentales y Metabolismo del Hemo, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Núñez, 1428, Buenos Aires, Argentina
Palabras clave:hexachlorobenzene; uroporphyrinogen decarboxylase; animal experiment; animal tissue; article; controlled study; drug toxicity; enzyme active site; enzyme modification; liver; nonhuman; porphyria; rat; Animal; Arginine; Binding Sites; Carboxy-Lyases; Diethyl Pyrocarbonate; Epoxy Compounds; Female; Hexachlorobenzene; Histidine; Hydroxylamine; Hydroxylamines; Liver; Methylene Blue; Photochemistry; Porphyria; Rats; Rats, Inbred Strains; Spectrophotometry; Support, Non-U.S. Gov't; Uroporphyrinogens; Animalia
Año:1991
Volumen:23
Número:7-8
Página de inicio:675
Página de fin:679
DOI: http://dx.doi.org/10.1016/0020-711X(91)90037-N
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:Arginine, 74-79-3; Carboxy-Lyases, EC 4.1.1.; Diethyl Pyrocarbonate, 1609-47-8; Epoxy Compounds; erythritol anhydride, 1464-53-5; Hexachlorobenzene, 118-74-1; Histidine, 71-00-1; Hydroxylamine, 7803-49-8; Hydroxylamines; Methylene Blue, 61-73-4; porphyrinogen carboxy-lyase, EC 4.1.1.-; Uroporphyrinogens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v23_n7-8_p675_DeCatabbi

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Citas:

---------- APA ----------
De Catabbi, S.B., De Molina, M.D.C.R. & De Viale, L.C.S.M. (1991) . Studies on the active centre of rat liver porphyrinogen carboxylyase in vivo effect of hexachlorobenzene. International Journal of Biochemistry, 23(7-8), 675-679.
http://dx.doi.org/10.1016/0020-711X(91)90037-N
---------- CHICAGO ----------
De Catabbi, S.B., De Molina, M.D.C.R., De Viale, L.C.S.M. "Studies on the active centre of rat liver porphyrinogen carboxylyase in vivo effect of hexachlorobenzene" . International Journal of Biochemistry 23, no. 7-8 (1991) : 675-679.
http://dx.doi.org/10.1016/0020-711X(91)90037-N
---------- MLA ----------
De Catabbi, S.B., De Molina, M.D.C.R., De Viale, L.C.S.M. "Studies on the active centre of rat liver porphyrinogen carboxylyase in vivo effect of hexachlorobenzene" . International Journal of Biochemistry, vol. 23, no. 7-8, 1991, pp. 675-679.
http://dx.doi.org/10.1016/0020-711X(91)90037-N
---------- VANCOUVER ----------
De Catabbi, S.B., De Molina, M.D.C.R., De Viale, L.C.S.M. Studies on the active centre of rat liver porphyrinogen carboxylyase in vivo effect of hexachlorobenzene. Int. J. Biochem. 1991;23(7-8):675-679.
http://dx.doi.org/10.1016/0020-711X(91)90037-N