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Abstract:

1. 1. Different porphobilinogen-deaminase (PBG-D) enzyme forms were found for D 27 and D 27/C6 (HEM R+) strains of Saccharomyces cerevisiae. 2. 2. PBG-D was partially purified and chromatographed on Sephadex G-100 in either the presence or absence of a protease inhibitor. For D 27 only one active peak was observed while for D 27/C6 strain two active peaks were found. 3. 3. A correlation between this differential behaviour and the presence of HEM R+ gene was looked for employing two segregants of one tetrad from D 27 and D 27/C6 mating. © 1991.

Registro:

Documento: Artículo
Título:Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour
Autor:Garcia, S.R.C.; Rossetti, M.V.; del Carmen Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPYP (CONICET F.C.E. y N., UBA), Ciudad Universitaria, Pabellon 11, 2do.piso, 1428 Buenos Aires, Argentina
Palabras clave:endogenous compound; porphobilinogen deaminase; article; fungus mutant; nonhuman; protein variant; saccharomyces cerevisiae; Ammonia-Lyases; Chromatography, Gel; Isoenzymes; Mutation; Saccharomyces cerevisiae; Support, Non-U.S. Gov't; Fungi; Saccharomyces cerevisiae
Año:1991
Volumen:23
Número:10
Página de inicio:1019
Página de fin:1023
DOI: http://dx.doi.org/10.1016/0020-711X(91)90139-E
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:Ammonia-Lyases, EC 4.3.1.; Isoenzymes; porphobilinogenase, EC 5.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v23_n10_p1019_Garcia

Referencias:

  • Anderson, Desnick, Purification and properties of Uroporphyrinogen I synthase from human erythrocytes (1980) J. biol. Chem., 255, pp. 1993-1999
  • Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analyt. Biochem., 72, pp. 248-254
  • Jordan, Berry, Mechanism of action of porphobilinogen-deaminase (1981) Biochem.J., 195, pp. 177-181
  • Jordan, Thomas, Warren, Purification, crystallization and properties of porpho-bilinogen deaminase from a recombinant strain of Escherichia coli K 12 (1988) Biochem.J., 254, pp. 427-435
  • Miyagi, Kaneshima, Nakada, Petryka, Watson, Uroporphyrinogen I synthase from human erythrocytes: separation, purification and properties of isoenzymes (1979) Proc. natn. Acad. Sci.U.S.A., 76, pp. 6172-6176
  • Rimington, Spectral absorption coefficients of some porphyrins in the Soret band region (1960) Biochem J, 75, pp. 620-623
  • Rossetti, Araujo, Lombardo, Correa García, Batlle, Porphyrin biosynthesis in Euglena gracilis VI. The effect of growth conditions on porphobilinogenase activity and further properties (1987) Comp. Biochem. Physiol., 87 B, pp. 593-600
  • Sancovich, Ferramola, Baffle, Grinstein, Preparation of porphobilinogen (1970) Methods in Enzymology, 17, pp. 220-222. , H. Tabor, C.W. Tabor, Academic Press, New York
  • Williams, Characterization of the multiple forms of hydroxymethylbilane synthase from rat spleen (1984) Biochem.J., 217, pp. 675-683

Citas:

---------- APA ----------
Garcia, S.R.C., Rossetti, M.V. & del Carmen Batlle, A.M. (1991) . Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour. International Journal of Biochemistry, 23(10), 1019-1023.
http://dx.doi.org/10.1016/0020-711X(91)90139-E
---------- CHICAGO ----------
Garcia, S.R.C., Rossetti, M.V., del Carmen Batlle, A.M. "Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour" . International Journal of Biochemistry 23, no. 10 (1991) : 1019-1023.
http://dx.doi.org/10.1016/0020-711X(91)90139-E
---------- MLA ----------
Garcia, S.R.C., Rossetti, M.V., del Carmen Batlle, A.M. "Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour" . International Journal of Biochemistry, vol. 23, no. 10, 1991, pp. 1019-1023.
http://dx.doi.org/10.1016/0020-711X(91)90139-E
---------- VANCOUVER ----------
Garcia, S.R.C., Rossetti, M.V., del Carmen Batlle, A.M. Different porphobilinogen-deaminase forms in wild and mutant strains of saccharomyces cerevisiae. A possible correlation with its segregants behaviour. Int. J. Biochem. 1991;23(10):1019-1023.
http://dx.doi.org/10.1016/0020-711X(91)90139-E