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1. 1. The sensitivity of partially purified low Km, phosphodiesterase (PDE) from Mucor rouxii to pharmacological agents and cAMP analogs was studied. The IC50 obtained were compared with those reported for PDEs from higher eukaryotes. 2. 2. The best inhibitors of the hydrolysis of 1 gmM cAMP were SQ 65.442 (IC50, c 10 μM), dipyridamol and CI 930. cGMP was not an inhibitor (IC50 > 1000 μm). 3. 3. The cAMP analogs were tested as inhibitors of the hydrolysis of 0. l μM cAMP. 8-Aminohexylamino cAMP was the best inhibitor with an IC50 of c 1 μM. 4. 4. A sedimentation profile of Mucor PDE was assayed in the presence of several pharmacological inhibitors and eAMP analogs. No isoforms with different sensitivity towards the inhibitors were detected. Forms with slightly different behaviour towards some cAMP analogs were observed. © 1990.


Documento: Artículo
Título:cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase
Autor:Tomes, C.; Moreno, S.
Filiación:Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:1 ethyl 4 hydrazino 1h pyrazolo[3,4 b]pyridine 5 carboxylic acid ethyl ester; 4 (3 butoxy 4 methoxybenzyl) 2 imidazolidinone; 4,5 dihydro 6 [4 (1h imidazol 1 yl)phenyl] 5 methyl 3(2h) pyridazinone; 6 [4 (3 methylureido)phenyl] 3(2h) pyridazinone; amrinone; cilostamide; cilostazol; cyclic amp; dipyridamole; etazolate; imazodan; isobutylmethylxanthine; milrinone; papaverine; phosphodiesterase; phosphodiesterase inhibitor; rolipram; sq 65442; theophylline; unclassified drug; article; fungus; mucor; nonhuman; 1-Methyl-3-isobutylxanthine; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Centrifugation, Density Gradient; Cyclic AMP; Cyclic GMP; Dipyridamole; Hydrolysis; Mucor; Nicotinic Acids; Papaverine; Pyridazines; Support, Non-U.S. Gov't; Theophylline; Amylomyces rouxii; Fungi; Mucor
Página de inicio:1047
Página de fin:1051
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
CAS:1-Methyl-3-isobutylxanthine, 28822-58-4; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC; 4,5-dihydro-6-(4-(imidazol-1-yl)phenyl)-5-methyl-3(2H)-pyridazinone, 86798-59-6; 8-aminohexylamino cAMP, 39824-30-1; Cyclic AMP, 60-92-4; Cyclic GMP, 7665-99-8; Dipyridamole, 58-32-2; Nicotinic Acids; Papaverine, 58-74-2; Pyridazines; SQ 65442, 50476-43-2; Theophylline, 58-55-9


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---------- APA ----------
Tomes, C. & Moreno, S. (1990) . cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase. International Journal of Biochemistry, 22(9), 1047-1051.
---------- CHICAGO ----------
Tomes, C., Moreno, S. "cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase" . International Journal of Biochemistry 22, no. 9 (1990) : 1047-1051.
---------- MLA ----------
Tomes, C., Moreno, S. "cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase" . International Journal of Biochemistry, vol. 22, no. 9, 1990, pp. 1047-1051.
---------- VANCOUVER ----------
Tomes, C., Moreno, S. cAMP analogs and selective inhibitors used to study low Km Mucor rouxii cAMP phosphodiesterase. Int. J. Biochem. 1990;22(9):1047-1051.