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Abstract:

1. 1. The aim of the present work is to shed light on the way of action of hexachlorobenzene (HCB) on hepatic ferrochelatase the mitochondrial enzyme which catalyzes the last step of haem biosynthetic pathway. 2. 2. Some properties of this enzyme from normal and HCB porphyric rat liver were studied. 3. 3. The present findings indicate that HCB treatment would modify the configuration of the enzyme perhaps allowing the active center of the porphyric ferrochelatase to be more exposed. 4. 4. As a consequence it would show: (a) its higher affinity for the iron; (b) the shorter time necessary to form the intermediate enzyme-substrate, reflected both by the existence of a shorter lag and consequently a shorter pre incubation time. 5. 5. However this modification elicited by the fungicide does not alter the submitochondrial distribution of the enzyme nor the optimal conditions for its measurement. © 1989.

Registro:

Documento: Artículo
Título:Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Studies on their properties
Autor:De Molina, M.C.R.; Taira, M.C.; De Viale, L.C.S.M.
Filiación:Laboratorio de Porfirias Experimentales y Metabolismo del Hemo, Departamento de Química Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Núñez, 1428, Buenos Aires, Argentina
Palabras clave:ferrochelatase; heme; hexachlorobenzene; animal cell; animal experiment; animal model; biological model; enzyme assay; enzyme kinetics; enzyme localization; female; hepatic porphyria; liver mitochondrion; nonhuman; normal value; oral drug administration; rat; Animal; Chlorobenzenes; Enzyme Stability; Ferrochelatase; Heme; Hexachlorobenzene; Hydrogen-Ion Concentration; Lyases; Mitochondria, Liver; Porphyria; Rats; Support, Non-U.S. Gov't
Año:1989
Volumen:21
Número:2
Página de inicio:219
Página de fin:226
DOI: http://dx.doi.org/10.1016/0020-711X(89)90112-2
Título revista:International Journal of Biochemistry
Título revista abreviado:Int. J. Biochem.
ISSN:0020711X
CODEN:IJBOB
CAS:ferrochelatase, 9012-93-5; heme, 14875-96-8; hexachlorobenzene, 118-74-1, 55600-34-5; Chlorobenzenes; Ferrochelatase, EC 4.99.1.1; Heme, 14875-96-8; Hexachlorobenzene, 118-74-1; Lyases, EC 4.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v21_n2_p219_DeMolina

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Citas:

---------- APA ----------
De Molina, M.C.R., Taira, M.C. & De Viale, L.C.S.M. (1989) . Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Studies on their properties. International Journal of Biochemistry, 21(2), 219-226.
http://dx.doi.org/10.1016/0020-711X(89)90112-2
---------- CHICAGO ----------
De Molina, M.C.R., Taira, M.C., De Viale, L.C.S.M. "Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Studies on their properties" . International Journal of Biochemistry 21, no. 2 (1989) : 219-226.
http://dx.doi.org/10.1016/0020-711X(89)90112-2
---------- MLA ----------
De Molina, M.C.R., Taira, M.C., De Viale, L.C.S.M. "Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Studies on their properties" . International Journal of Biochemistry, vol. 21, no. 2, 1989, pp. 219-226.
http://dx.doi.org/10.1016/0020-711X(89)90112-2
---------- VANCOUVER ----------
De Molina, M.C.R., Taira, M.C., De Viale, L.C.S.M. Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. Studies on their properties. Int. J. Biochem. 1989;21(2):219-226.
http://dx.doi.org/10.1016/0020-711X(89)90112-2